Abstract
Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase) gene from the diatomCyclotella cryptica. The recombinant DNA integrated into one or more random sites within the algal genome and the foreign protein was produced by the algal transformants. This is the first report of genetic transformation of any chlorophyll c-containing microalgal strain. We are using this system to introduce additional copies of the ACCase gene into diatoms in an attempt to manipulate lipid accumulation in transformed strains.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Roessler, P. G. (1990),J. Phycol. 26, 393–399.
Shay, E. G. (1993),Biomass and Bioenergy 4, 227–242.
Kindle, K. L. and Sodeinde, O. A. (1994),J. Appl. Phycol. 6, 231–238.
Schiedlmeier, B., Schmitt, R., Mueller, W., Kirk, M. M., Gruber, H., Mages, W., and Kirk, D. L. (1994),Proc. Natl. Acad. Sci. USA 91, 5080–5084.
Round, F. E., Crawford, R. M., and Mann, D. G. (1990),The Diatoms. Cambridge University Press, Cambridge, UK, p. 747.
Reimann, B. E. F., Lewin, J. M. C., and Guillard, R. R. L. (1963),Phycologia 3, 75–84.
Barclay, W., Johansen, J., Chelf, P., Nagle, N., Roessler, P., and Lemke, P. (1986), Microalgae Culture Collection 1986–1987, Solar Energy Research Institute, Golden, CO, SERI/SP-232-3079.
Brown, L. (1982),Phycologia 21, 408–410.
Dunahay, T. G., Jarvis, E. E., and Roessler, P. G. (1995),J. Phycol. 31, 1004–1012.
Roessler, P. G. and Ohlrogge, J. B. (1993),J. Biol. Chem. 268, 19,254-19,259.
Sanford, J. C., Smith, F. D., and Russell, J. A. (1993),Methods Enzymol. 217, 483–509.
Jarvis, E. E., Dunahay, T. G., and Brown, L. M. (1992),J. Phycol. 28, 356–362.
Lion, T. and Haas, O. A. (1990),Anal. Biochem. 188, 335–337.
Fromm, M. E., Taylor, L. P., and Walbot, V. (1986),Nature 319, 791–793.
Gruber, M. Y. and Crosby, W. L. (1993), InMethods in Plant Molecular Biology and Biotechnology (Glick, B. R. and Thompson, J. E., eds.), CRC, Boca Raton, FL, pp. 89–119.
Post-Beittenmiller, D., Roughan, G., and Ohlrogge, J. B. (1992),Plant Physiol. 100, 923–930.
Roessler, P. G. (1988),Arch. Biochem. Biophys. 267, 521–528.
Dunahay, T. G. (1993),BioTechniques 15, 452–460.
Ow, D. W., Wood, K. V., DeLuca, M., de Wet, J. R., Helinski, D. R., and Howell, S. H. (1986),Science 234, 856–859.
Jarvis, E. E. and Brown, L. M. (1991),Curr. Genet. 19, 317–321.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dunahay, T.G., Jarvis, E.E., Dais, S.S. et al. Manipulation of microalgal lipid production using genetic engineering. Appl Biochem Biotechnol 57, 223–231 (1996). https://doi.org/10.1007/BF02941703
Issue Date:
DOI: https://doi.org/10.1007/BF02941703