Abstract
The arylsulfatase gene (astA) from Pseudoalteromonas carrageenovora genome was subcloned into pHCEIA vector, in which the hyper constitutive expression (HCE) promoter from the d-amino acid aminotransferase (DAAT) gene of Geobacillus toebii was employed. When the constructed pHCE-AST was introduced into E. coli, the transformant showed the hydrolyzing activity for 4-methylumbelliferyl-sulfate and p-nitrophenyl-sulfate. When the cell was cultured on fermentor containing MaxyBroth-HD medium with 1% glycerol, the enzyme activity reached 12.8 unit/mL. On MaxyBroth-HD medium with 2% glycerol, the cell showed 2.7-fold higher arylsulfatase expression than that with 1% glycerol. The fed-batch cultivation employing MaxyBroth-HD medium and additional feeding of glycerol gave about 143 unit/mL of arylsulfatase at 20 h, which corresponds to 4-fold higher enzyme activity than that of 2% glycerol batch culture. Most of arylsulfatase activity in fed-batch culture was produced in the extracellular medium, whereas the activity in the batch cultures was localized in the periplasmic cell space.
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References
M. Duckworth and W. Yaphe, Carbohydr. Res., 16, 189 (1971).
G.G. Allan, P.G. Johnson, Y. Lay and K.V. Sarkanen, Carbohydr. Res., 17, 234 (1971).
M.R. S. Melo, J. P. A. Feitosa, A. L. P. Freitas and R.C. M. de Paula, Carbohydr. Polym., 49, 491(2002).
D. Renn, Trends Biotechnol., 15, 9 (1997).
H. S. Yoon and Y. H. Park, Bull Kor. Fish. Soc., 24, 27 (1984).
R. B. Camden, USA Patent, US086751 (1985).
H. J. Seo, J.H. Kim, D. S. Byun and H. R. Kim, Fish. Sci., 58, 1575 (2001).
S. Amott, J. Mol. Biol., 90, 269 (1974).
C. Miech, T. Dierks, T. Selmer, K. von Figura and B. Schmidt, J. Biol. Chem., 273, 4835 (1998).
M. J. Henderson and F. H. Milazzo, J. Bacteriol., 139, 80 (1979).
Y. Murooka and T. Harada, J. Biol. Chem., 145, 796 (1981).
Y. Murooka, M. H. Yim and T. Harada, Appl. Environ. Microbiol., 39, 812 (1980).
G. Delisle and F. H. Milazzo, Biochim. Biophys. Acta, 212, 505 (1970).
A. A. Milanesi and J.W. C. Bind, Comp. Biochem. Physiol., 41, 473 (1972).
T. Barbeyron, P. Potin, C. Richard, O. Collin and B. Kloareg, Microbiology, 141, 2897 (1995).
P. Haryonug, J. J. Song, S. P. Hong, Y. H. Choi, S.W. Yun, J.H. Kim, S. C. Lee, S. G. Lee and M. H. Sung, Biotechnol. Lett., 24, 1185 (2002).
J. M. Lim, Y. H. Jang, H. R. Kim, Y. T. Kim, T. J. Choi, J. K. Kim and S.W. Nam, J. Microbiol. Biotechnol., 14, 777 (2004).
D. E. Kim, K. H. Kim, Y. J. Bae, J. H. Lee, Y. H. Jang and S.W. Nam, Protein Expr. Purif., 39, 107 (2005).
M. J. Kim, Y.H. Jang, M. H. Sung, Y. H. Kim and S.W. Nam, Kor. J. Microbiol. Biotechnol., 35, 11 (2007).
P. Simon, S. M. Richter and V. B. Urlacher, J. Biotechnol., 129, 481 (2007).
S. K. Lee and D. Keasling, Biotechnol. Prog., 22, 1547 (2006).
M.A. Nesmeyanova, A. E. Kalinin, A. L. Karamyshev, N. I. Mikhaleva and V. I. Krupyanko, Process Biochem., 32, 1 (1997).
I. Helianti, N. Nurhayati, M. Ulfah, B. Wahyuntari and S. Setyahadi, J. Biomed. Biotechnol., 2010, 980567 (2010).
J. Siurkus, J. Panula-Perälä, U. Horn, M. Kraft, R. Rimseliene and P. Neubauer, Microb. Cell Fact., 9, 35 (2010).
Y. J. Son, J.Y. Bae, S. H. Chong, H. S. Lee, S. H. Mo, T.Y. Kim and H. Choe, Appl. Biochem. Biotechnol., 162, 1585 (2010).
K. J. Jeong, J. H. Choi, W. M. Yoo, K. C. Keum, N. C. Yoo, S.Y. Lee and M. H. Sung, Protein Expr. Purif., 36, 150 (2004).
H. Jiang, L. Shang, S.H. Yoon, S.Y. Lee and Z. Yu, Biotechnol. Lett., 28, 1241 (2006).
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Kim, MJ., Kim, JH. & Nam, SW. Constitutive overexpression of Pseudoalteromonas carrageenovora arylsulfatase in E. coli fed-batch culture. Korean J. Chem. Eng. 28, 1101–1104 (2011). https://doi.org/10.1007/s11814-010-0488-9
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DOI: https://doi.org/10.1007/s11814-010-0488-9