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Enhancement of glucose isomerase activity by pretreatment with substrates prior to immobilization

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Abstract

To improve the activity of covalently immobilized glucose isomerase, the effects of glucose isomerase pretreatment with d-glucose or d-xylose prior to immobilization were investigated. Glucose isomerase was pretreated with d-glucose or d-xylose to prevent loss of activity, followed by immobilization on a silica gel surface. Pretreated immobilized glucose isomerase (PIGI) with 2.0M d-xylose (194.0 U/g matrix) had higher activity than PIGIs with d-glucose. The optimal temperature, reaction time, and agitation speed for glucose isomerase pretreatment were 60 oC, 45 min, and 200 rpm, respectively. Consequently, the activity of PIGI with d-xylose was 254.9 U/g matrix, which is about 2.5 times higher than that of non-pretreated immobilized glucose isomerase (non-PIGI). PIGI also showed better reusability than non-PIGI, with 75.2% of its original activity being retained after 10 reuses.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, 5 Anam-dong, Sungbuk-gu, Seoul, 136-701, Korea

    Yoon Seok Song, Ji Eun Kim & Seung Wook Kim

  2. Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, Seoul, 139-701, Korea

    Chulhwan Park

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  1. Yoon Seok Song
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  2. Ji Eun Kim
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  3. Chulhwan Park
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  4. Seung Wook Kim
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Correspondence to Seung Wook Kim.

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Song, Y.S., Kim, J.E., Park, C. et al. Enhancement of glucose isomerase activity by pretreatment with substrates prior to immobilization. Korean J. Chem. Eng. 28, 1096–1100 (2011). https://doi.org/10.1007/s11814-010-0464-4

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  • DOI: https://doi.org/10.1007/s11814-010-0464-4

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