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Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity

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Abstract

Lipase was pretreated with soybean oil in order to allow fatty acids to bond to the active site before immobilization. This pretreated lipase exhibited steric hindrance around the active site such that during immobilization, covalent bonds were formed between the carrier and the lipase region far from the active site. The activity of the pretreated lipase immobilized covalently on a silica gel was 530 U/g-matrix, which is 16 times higher than that of the immobilized non-pretreated lipase. In addition, the immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

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Acknowledgment

This work was supported by research grant 2003-N-BI03-P-01 from the Korea Energy Management Corporation.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, 1, Anam-dong, 136-701, Sungbuk-ku, Seoul, Korea

    Dong Hwan Lee, Jung Mo Kim, Ji Won Lee & Seung Wook Kim

  2. Research Institute of Engineering and Technology, Korea University, 1, Anam-dong, 136-701, Sungbuk-ku, Seoul, Korea

    Seong Woo Kang

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  1. Dong Hwan Lee
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  2. Jung Mo Kim
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  3. Seong Woo Kang
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  4. Ji Won Lee
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Correspondence to Seung Wook Kim.

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Lee, D.H., Kim, J.M., Kang, S.W. et al. Pretreatment of lipase with soybean oil before immobilization to prevent loss of activity. Biotechnol Lett 28, 1965–1969 (2006). https://doi.org/10.1007/s10529-006-9181-9

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  • DOI: https://doi.org/10.1007/s10529-006-9181-9

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