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Rapid differentiation of new isolates with MALDI-TOF mass spectrometry via discriminant function analysis based on principal components

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Abstract

Discriminant function analysis based on principal components was applied to the spectral outputs of whole cell suspensions of nine isolates from matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. First, based on the salt tolerance and whole cell proteins, the similarity of the isolates to moderate halophiles was established. Intact microorganisms were then inferentially clustered by MALDI-TOF mass spectroscopy taking four type strains as precursors. Two of these type strains were moderate halophilic bacteria (Halomonas salina and Halomonas halophila), one was a mesophilic bacteria (Escherichia coli), and one was a halophilic archaea (Haloarcula vallismortis). Results showed that the isolates were significantly similar to halophiles but were different from a mesophile. This investigation demonstrates the feasibility of using whole cell suspensions for rapid differentiation prior to extensive experimentation.

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Authors and Affiliations

  1. Department of Bioengineering, Marmara University, Kadikoy, 34722, Istanbul, Turkey

    Berna Sariyar-Akbulut

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  1. Berna Sariyar-Akbulut
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Correspondence to Berna Sariyar-Akbulut.

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Sariyar-Akbulut, B. Rapid differentiation of new isolates with MALDI-TOF mass spectrometry via discriminant function analysis based on principal components. Korean J. Chem. Eng. 26, 1645–1651 (2009). https://doi.org/10.1007/s11814-009-0243-2

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  • DOI: https://doi.org/10.1007/s11814-009-0243-2

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