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Characterization of a carbon composite electrode for an electrochemical immunosensor

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Abstract

A bioactive platform with a carbon composite electrode was developed for rapid detection of Escherichia coli O157:H7. The porous carbon composite electrode was prepared by a sol-gel method with a mixture of graphite powder and tetraethyl orthosilicate/ethanol. Escherichia coli O157:H7 antibodies were physically adsorbed onto the carbon composite electrode. Direct measurements by cyclic voltammetry and electrochemical impedance spectroscopy in the presence of [Fe(CN)6]3−/4− as a redox probe showed that the immobilization of antibodies onto the carbon composite electrode surface and the binding of Escherichia coli O157:H7 cells with antibodies systematically increased the electron-transfer resistance. Those results suggest that a sol-gel derived graphite composite electrode might be utilized as a label-free electrochemical immunosensor for diagnosis, biochemical research, food industry, and so on.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering and Interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul, 121-742, Korea

    Eun Chan Cho, Byong Ok Jang, Eui Jung Kim & Kee-Kahb Koo

  2. Department of Chemical Engineering, University of Ulsan, Ulsan, 680-749, Korea

    Eui Jung Kim

Authors
  1. Eun Chan Cho
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  2. Byong Ok Jang
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  3. Eui Jung Kim
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  4. Kee-Kahb Koo
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Correspondence to Kee-Kahb Koo.

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Cho, E.C., Jang, B.O., Kim, E.J. et al. Characterization of a carbon composite electrode for an electrochemical immunosensor. Korean J. Chem. Eng. 25, 548–552 (2008). https://doi.org/10.1007/s11814-008-0092-4

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  • DOI: https://doi.org/10.1007/s11814-008-0092-4

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