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Development of an Electrochemical Immunosensor Based on Multi-functional Carboxylated Multiwalled Carbon Nanotube–Silver Nanoparticles-Chitosan Film for Rapid Determination of Ractopamine

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Abstract

A porous electrochemical immunosensor was fabricated for the sensitive determination of ractopamine (RAC) based on a cMWCNT–Ag–CS matrix film with a working bare screen-printed carbon electrode (SPCE). At the same time, the amine groups of chitosan interacted with the cMWCNT and silver nanoparticles, which was examined in detail by XPS. Cyclic voltammetry (CV) was employed to investigate the electrochemical characteristics of the immunosensor. Under optimal conditions, the logarithmic value of CRAC was positively correlated with the △Ipa of the RAC immunosensor, from 0.01 to 100 ng mL−1 (correlation coefficient R2 = 0.998). The limit of detection (LOD, 3σ) was 0.001 ng mL−1. The relative standard deviation (RSD) for Ipa current was 3.78%. These results suggested that the cMWCNT–Ag–CS matrix film provides a microenvironment beneficial for the coupling probability of anti-RAC, promotes electron transfer, and improves the sensitivity of the immunosensor. This strategy based on a cMWCNT–Ag–CS substrate may offer a new approach for the sensitive and selective detection of other lean meat powders via only changing the corresponding antibodies.

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Funding

The authors thank the National Science and Technology Council for supporting this work. (MOST 110-2222-E-606-001).

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  1. Department of Chemical and Materials Engineering, Chung Cheng Institute of Technology, National Defense University, Taoyuan, 33551, Taiwan

    Wen-Chien Huang & You-Ning Hsiung

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  1. Wen-Chien Huang
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WCH: Conceptualization, Methodology, Investigation, Data curation, Analysis and interpretation of data, Writing-original draft, writing-review and edition. YNH: Acquisition of data.

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Correspondence to Wen-Chien Huang.

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Huang, WC., Hsiung, YN. Development of an Electrochemical Immunosensor Based on Multi-functional Carboxylated Multiwalled Carbon Nanotube–Silver Nanoparticles-Chitosan Film for Rapid Determination of Ractopamine. J Polym Environ (2023). https://doi.org/10.1007/s10924-023-03088-2

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