Abstract
Crude proteolytic enzyme was extracted from papaya latex using two solvents, water and phosphate buffer pH 6. The yield of extracted enzyme using water as a solvent was similar to that using phosphate buffer. Following the solvent extraction, the extracted enzyme was precipitated in 45 wt% saturated ammonium sulfate solution. The yield and activity of precipitated enzyme considerably decreased. Crude proteolytic enzyme extracted using water as an extracting liquid was, therefore, selected to use in gelatin production from raw hide hydrolysis, comparing to the use of commercial papain. The effects of hydrolysis conditions on gelatin recovery and properties of obtained gelatin were investigated. The optimum conditions for the activities of both crude extracted enzyme and commercial papain were at 75 °C and pH 7. At this condition, the highest percentages of gelatin recovery were obtained from raw hide hydrolysis reactions. The gelatin recovery and gel strength of gelatin obtained from crude extracted enzyme and commercial papain hydrolysis were similar. This proved that crude extracted enzyme from papaya latex could be effectively used in gelatin production, instead of the use of commercial papain, with a comparatively low cost.
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References
Aspmo, S. I., Horn, S. J. and Eijsink, V.G. H., “Enzymatic hydrolysis of Atlantic cod (Gadus morhua L.) viscera,” Process Biochem., 40, 1957 (2005).
Belter, P., Cussler, E. L. and Hu, W., Bioseparation, John Wiley & Sons, New York (1998).
BS EN ISO 9665:2000. “Adhesives-animal glues-methods of sampling and testing (ISO 9665:1998)” (2000).
Chomarat, N., Robert, L., Seris, J. L. and Kern, P., “Comparative efficiency of pepsin and proctase for the preparation of bovine skin gelatin,” Enzyme Microb. Technol., 16, 756 (1994).
Damrongsakkul, S., Ratanathammapan, K., Komolpis, K. and Tanthapanichakoon, W., “Enzymatic hydrolysis of raw hide using papain and nuetrase,” to be submitted to Bioresource Technol. (2006).
Friess, W., “Collagen — biomaterial for drug delivery,” Eur. J. Pharm. Biopharm., 45, 113 (1998).
Gomez-Guillen, M. C., Gimenze, B. and Montero, P., “Extraction of gelatin from fish skins by high pressure treatment,” Food Hydrocolloid, 19, 1 (2005).
Guerard, F., Dufosse, L., De La Broise, D. and Binet, A., “Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacares) wastes using alcalase,” J. Mol. Catal. B-Enzym., 11, 1051 (2001).
Oh, K. D. and Kim, C., “A study on enzymatic hydrolysis of cellulose in an attrition bioreactor,” Korean J. Chem. Eng., 4, 105 (1987).
Park, C.Y., Ryu, Y.W. and Kim, C., “Kinetics and rate of enzymatic hydrolysis of cellulose in supercritical carbon dioxide,” Korean J. Chem. Eng., 18, 475 (2001).
Pitpreecha, S., Gelatin production from large animal raw hide using proteolytic enzyme extracted from papaya latex, Master Dissertation, Department of Chemical Engineering, Faculty of Engineering, Graduate School, Chulalongkorn University (2006).
Ratanathammapan, K., Effects of enzymatic conditions on gelatin production from raw hide, Master Dissertation, Department of Chemical Engineering, Faculty of Engineering, Graduate School, Chulalongkorn University (2005).
Sharphouse, J. H., Applied protein chemistry, Applied Science Publishers, London (1980).
Simeonova, L. S. and Dalev, P. G., “Utilization of a leather industry waste,” Waste Manage., 16, 765 (1996).
Taylor, M. M., Diefendorf, E. J., Foglia, T. A., Bailey, D.G. and Feairheller, S.H., “Enzymatic treatment of offal from fleshing machines,” JALA, 84, 71 (1989).
Walker, J.M., The protein protocols handbook, Humana Press, New Jersey (1996).
Walsh, G., Proteins: Biochemistry and biotechnology, John Wiley & Sons, New York (2002).
Wuttiwiangtham, T., Purification and characterization of papain from latex of papaya (Carica papaya Linn.), Master Dissertation, Department of Biotechnology, Faculty of Science, Graduate School, Chulalongkorn University (1993).
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Pitpreecha, S., Damrongsakkul, S. Hydrolysis of raw hide using proteolytic enzyme extracted from papaya latex. Korean J. Chem. Eng. 23, 972–976 (2006). https://doi.org/10.1007/s11814-006-0017-z
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DOI: https://doi.org/10.1007/s11814-006-0017-z