Abstract
Staphylococcus aureus is a common marine foodborne pathogen. In this study, antibiotics ciprofloxacin and enrofloxacin were used to induce drug-resistance in S. aureus. The differentially expressed proteins (DEPs) were analyzed and compared with those in the bacteria cultured without antibiotics. The primary proteomic alterations were in the levels of cell membrane components and proteins related to lysine and folic acid biosynthesis, which were all significantly up-regulated. The minimal inhibitory concentrations (MIC) for both test drugs were elevated to 10 µg mL−1 following serial passaging. These results indicated that, for both ciprofloxacin and enrofloxacin, drug-resistance were developed even in the subinhibitory levels and the primary response was a major alteration in the cell membrane proteome. These changes were similar to those observed in S. aureus cultured with super-MIC levels of these antibiotics. The current study provides a theoretical basis for in-depth study of the related changes of marine foodborne pathogens in subinhibitory concentrations that are commonly found in situ.
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This research was funded by the Professional Innovation and Integration Project of Qingdao University (2020).
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Xu, X., Lin, H., Guo, J. et al. Subinhibitory Levels of Fluoroquinolones Result in Enrichment of the Membrane Proteome of Staphylococcus aureus. J. Ocean Univ. China 22, 1439–1445 (2023). https://doi.org/10.1007/s11802-023-5489-5
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DOI: https://doi.org/10.1007/s11802-023-5489-5