Skip to main content
SpringerLink
Log in

Productive Traits and Triploid Rate Stability in Triploidy-Induced ‘Haida No. 2’ Strain of the Pacific Oyster, Crassostrea gigas

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

In order to evaluate the effects of triploidy induction on a selected strain ‘Haida No. 2’ of the Pacific oyster Crassostrea gigas, which is characterized with golden shell color and high growth rate, the growth, survival rate and stability of triploid rate were analyzed at different development stages in the present study. Three different conditions inhibiting the release of polar body II or polar body I were tested: (A) Cytochalasin-B (CB), 0.5 mg L−1 at 10 min post-insemination for 15 min; (B) CB, 0.5 mg L−1at 15 min post-insemination for 20 min; and (C) CB, 0.7 mg L−1, at 15 min post-insemination for 20 min. The triploidy induction treatments significantly reduced the D-larvae and survival rates at the larvae stage but not at the juvenile and adult stages. Triploid rate dramatically decreased at the larval stage and did not significantly change at the juvenile and adult stages. Regarding the stability of the triploid rate, there was a significant difference between the three treatment groups. Larvae from the treatment A and control groups exhibited higher growth rates in shell height than those from the other two treatment groups at day 27. Triploid juveniles and adults from the treatment A group exhibited a higher wet weight than diploids from the control group and triploids from the other treatment groups. Triploidy induction did not affect the shell color of the progeny. The results obtained in the study demonstrate that triploidy induction has the potential to be used to increase the production of C. gigas variety ‘Haida No. 2’ without modifying its golden shell color.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allen Jr., S. K., and Downing, S. L., 1986. Performance of triploid Pacific oysters, Crassostrea gigas (Thunberg). I. Survival, growth, glycogen content, and sexual maturation in yearlings. Journal of Experimental Marine Biology and Ecology, 102: 197–208.

    Article  Google Scholar 

  • Benfey, T. J., 1999. The physiology and behavior of triploid fishes. Reviews in Fisheries Science, 7: 39–67

    Article  Google Scholar 

  • Buestel, D., Ropert, M., Prou, J., and Goulletquer, P., 2009. History, status, and future of oyster culture in France. Journal of Shellfish Research, 28: 813–820.

    Article  Google Scholar 

  • Callam, B. R., Allen Jr., S. K., and Frank-Lawale, A., 2016. Genetic and environmental influence on triploid Crassostrea virginica grown in Chesapeake Bay: Growth. Aquaculture, 452: 97–106.

    Article  Google Scholar 

  • Cheney, D. P., 2000. Summer mortality of Pacific oysters, Crassostrea gigas (Thunberg): Initial finding on multiple environmental stressors in Puget Sound, Washington, 1998. Journal of Shellfish Research, 19: 353–359.

    Google Scholar 

  • Desrosiers, R. R., Gérard, A., Peignon, J. M., Naciri, Y., Dufresne, L., Morasse, J., et al., 1993. A novel method to produce triploids in bivalve molluscs by the use of 6-dimethylaminopurine. Journal of Experimental Marine Biology and Ecology, 170: 29–43.

    Article  Google Scholar 

  • Garnier-Géré, P. H., Naciri-Graven, Y., Bougrier, S., Magoulas, A., Héral, M., Kotoulas, G., et al., 2002. Influences of triploidy, parentage and genetic diversity on growth of the Pacific oyster Crassostrea gigas reared in contrasting natural environments. Molecular Ecology, 11: 1499–1514.

    Article  Google Scholar 

  • Ge, J. L., 2015. Selective breeding and genetic analysis of shell color traits in the Pacific oyster, Crassostrea gigas. PhD thesis. Ocean University of China (in Chinese with English abstract).

  • Gérard, A., Ledu, C., Phélipot, P., and Naciri-Graven, Y., 1999. The induction of MI and MII triploids in the Pacific oyster Crassostrea gigas with 6-DMAP or CB. Aquaculture, 174: 229–242.

    Article  Google Scholar 

  • Gerard, A., Naciri, Y., Peignon, J. M., Ledu, C., and Phelipot, P., 1994. Optimization of triploid induction by the use of 6-DMAP for the oyster Crassostrea gigas (Thunberg). Aquaculture Research, 25: 709–719.

    Article  Google Scholar 

  • Guo, X. M., and Allen Jr., S. K., 1994. Reproductive potential and genetics of triploid Pacific oysters, Crassostrea gigas (Thunberg). The Biological Bulletin, 187: 309–318.

    Article  Google Scholar 

  • Guo, X. M., Cooper, K., Hershberger, W. K., and Chew, K. K., 1992. Genetic consequences of blocking polar body I with cytochalasin B in fertilized eggs of the Pacific oyster, Crassostrea gigas: I. Ploidy of resultant embryos. The Biological Bulletin, 183: 381–386.

    Article  Google Scholar 

  • Hawkins, A. J. S., Day, A. J., Gérard, A., Naciri, C., Ledu, C., Bayne, B. L., et al., 1994. A genetic and metabolic basis for faster growth among triploids induced by blocking meiosis I but not meiosis II in the larviparous European flat oyster, Ostrea edulis L. Journal of Experimental Marine Biology and Ecology, 184: 21–40.

    Article  Google Scholar 

  • Houssin, M., Trancart, S., Denechere, L., Oden, E., Adeline, B., Lepoitevin, M., et al., 2019. Abnormal mortality of triploid adult Pacific oysters: Is there a correlation with high gametogenesis in Normandy, France?. Aquaculture, 505: 63–71.

    Article  Google Scholar 

  • Jeung, H. D., Keshavmurthy, S., Lim, H. J., Kim, S. K., and Choi, K. S., 2016. Quantification of reproductive effort of the triploid Pacific oyster, Crassostrea gigas raised in intertidal rack and bag oyster culture system off the west coast of Korea during spawning season. Aquaculture, 464: 374–380.

    Article  Google Scholar 

  • Kong, L. F., Song, S. L., and Li, Q., 2017. The effect of interstrain hybridization on the production performance in the Pacific oyster Crassostrea gigas. Aquaculture, 472: 44–49.

    Article  Google Scholar 

  • Li, Q., Wang, Q. Z., Liu, S. K., and Kong, L. F., 2011. Selection response and realized heritability for growth in three stocks of the Pacific oyster Crassostrea gigas. Fisheries Science, 77: 643–648.

    Article  Google Scholar 

  • Melo, E. M. C., Gomes, C. H. A. M., Silva, F. C. D., Sühnel, S., and Melo, C. M. R. D., 2015. Chemical and physical methods of triploidy induction in Crassostrea gigas (Thunberg, 1793). Boletim do Instituto de Pesca, 414: 889–898.

    Google Scholar 

  • Nell, J. A., 2001. The history of oyster farming in Australia. Marine Fisheries Review, 63: 14–25.

    Google Scholar 

  • Nell, J. A., 2002. Farming triploid oysters. Aquaculture, 210: 69–88.

    Article  Google Scholar 

  • Nell, J. A., and Perkins, B., 2005. Studies on triploid oysters in Australia: Farming potential of all-triploid Pacific oysters, Crassostrea gigas (Thunberg), in Port Stephens, New South Wales, Australia. Aquaculture Research, 36: 530–536.

    Article  Google Scholar 

  • Qin, Y. P., Zhang, Y. H., Ma, H. T., Wu, X. W., Xiao, S., Li, J., et al., 2018. Comparison of the biochemical composition and nutritional quality between diploid and triploid Hong Kong oysters, Crassostrea hongkongensis. Frontiers in physiology, 9: 1674.

    Article  Google Scholar 

  • Qin, Y. P., Zhang, Y. H., Mo, R. G., Zhang, Y., Li, J., Zhou, Y. L., et al., 2019. Influence of ploidy and environment on grow-out traits of diploid and triploid Hong Kong oysters Crassostrea hongkongensis in southern China. Aquaculture, 507: 108–118.

    Article  Google Scholar 

  • Smith, I. R., Nell, J. A., and Adlard, R., 2000. The effect of growing level and growing method on winter mortality, Mikrocytos roughleyi, in diploid and triploid Sydney rock oysters, Saccostrea glomerata. Aquaculture, 185: 197–205.

    Article  Google Scholar 

  • Stanley, J. G., Hidu, H., and Allen Jr., S. K., 1984. Growth of American oysters increased by polyploidy induced by blocking meiosis I but not meiosis II. Aquaculture, 37: 147–155.

    Article  Google Scholar 

  • Supan, J. E., Wilson, C. E., and Allen Jr., S. K., 2000. The effect of cytochalasin B dosage on the survival and ploidy of Crassostrea virginica (Gmelin) larvae. Journal of Shellfish Research, 19: 125–128.

    Google Scholar 

  • Toro, J. E., Sanhueza, M. A., Paredes, L., and Canello, F., 1995. Induction of triploid embryos by heat shock in the Chilean northern scallop Argopecten purpuratus Lamarck, 1819. New Zealand Journal of Marine and Freshwater Research, 29: 101–105.

    Article  Google Scholar 

  • Wadsworth, P., Wilson, A. E., and Walton, W. C., 2019. A meta-analysis of growth rate in diploid and triploid oysters. Aquaculture, 499: 9–16.

    Article  Google Scholar 

  • Wang, Z. P., Guo, X. M., Allen Jr., S. K., and Wang, R. C., 2002. Heterozygosity and body size in triploid Pacific oysters, Crassostrea gigas Thunberg, produced from meiosis II inhibition and tetraploids. Aquaculture, 204: 337–348.

    Article  Google Scholar 

  • Yang, H. P., and Guo, X. M., 2006. Polyploid induction by heat shock-induced meiosis and mitosis inhibition in the dwarf surfclam, Mulinia lateralis Say. Aquaculture, 252: 171–182.

    Article  Google Scholar 

  • Zouros, E., Romero-Dorey, M., and Mallet, A. L., 1988. Heterozygosity and growth in marine bivalves: Further data and possible explanations. Evolution, 42: 1332–1341.

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the grants from the China Agriculture Research System Project (No. CARS-49), and the Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province (Nos. 2020LZGC016, 2021 LZGC027).

Author information

Authors and Affiliations

  1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Yongguo Li, Qi Li, Ye Liu & Chengxun Xu

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Qi Li

Authors
  1. Yongguo Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ye Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chengxun Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qi Li.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, Y., Li, Q., Liu, Y. et al. Productive Traits and Triploid Rate Stability in Triploidy-Induced ‘Haida No. 2’ Strain of the Pacific Oyster, Crassostrea gigas. J. Ocean Univ. China 22, 229–234 (2023). https://doi.org/10.1007/s11802-023-5183-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-023-5183-7

Key words

Search

Navigation