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Effects of temperature and salinity on the larval early development, growth, and settlement of the diploid, triploid, and tetraploid Pacific oyster “Haida No. 2” strain

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Abstract

Tetraploids play a crucial role in the large-scale production of all-triploid Pacific oyster, Crassostrea gigas by mating with diploids. For nursery farms, research related to larval tolerance to the environment appears to be extremely important. For this goal, fertilization, embryonic development, growth, and settlement from fertilization stage to metamorphosis stage of the diploid, triploid, and tetraploid larvae of C. gigas “Haida No. 2” strain were studied under controlled conditions of temperature (20, 24, 28, and 32 ℃) and salinity (18, 21, 24, 27, and 30 psu). The results showed that increasing temperature and lowering salinity reduced survival time of sperm while increasing temperature and raising salinity accelerated the germinal vesicle breakdown (GVBD) ratio of stripped eggs in diploids and tetraploids for the first time. The appropriate condition for hatching of four crosses (DD, DT, TD, and TT, males were listed first, D for diploid, T for tetraploid) were listed as follows: 20–28 ℃/27–30 psu for DD, 24 ℃/27–30 psu for DT, 24–28 ℃/27–30 psu for TD, and 24 ℃/27–30 psu for TT. Diploids and triploids had similar performance but were all better than tetraploids under different temperature and salinity. The settlement rate increased with rising temperature while salinity and ploidy had little influence on settlement. The information obtained in this study can contribute to increasing the yield of triploid and tetraploid “Haida No. 2” of C. gigas.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by grants from the National Key R&D Program of Shandong Province (2022LZGCQY010, 2021ZLGX03, and 2021TSGC1240), and China Agriculture Research System Project (CARS-49).

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  1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Geng Cheng, Yuanxin Liang, Haining Zhang, Chengxun Xu & Qi Li

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Qi Li

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Geng Cheng: conceptualization, supervision, resources, project admi. Yuanxin Liang: resources. Haining Zhang: resources. Chengxun Xu: resources. Qi Li: conceptualization, supervision, resources, project admi.

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Correspondence to Qi Li.

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The present study was performed according to the standard operation procedures (SOPs) of the Guide for the Use of Experimental Animals of the Ocean University of China. All animal care and use procedures were approved by the Institutional Animal Care and Use Committee of Ocean University of China.

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Cheng, G., Liang, Y., Zhang, H. et al. Effects of temperature and salinity on the larval early development, growth, and settlement of the diploid, triploid, and tetraploid Pacific oyster “Haida No. 2” strain. Aquacult Int (2024). https://doi.org/10.1007/s10499-024-01457-9

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