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Enhanced Biodegradation of High-Salinity and Low-Temperature Crude-Oil Wastewater by Immobilized Crude-Oil Biodegrading Microbiota

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Abstract

High salt and low temperature are the bottlenecks for the remove of oil contaminants by enriched crude-oil degrading microbiota in Liaohe Estuarine Wetland (LEW), China. To improve the performance of crude-oil removal, microbiota was further immobilized by two methods, i.e., sodium alginate (SA), and polyvinyl alcohol and sodium alginate (PVA+SA). Results showed that the crude oil was effectively removed by the enrichment with an average degrading ratio of 19.42–31.45 mg(L d)−1. The optimal inoculum size for the n-alkanes removal was 10% and 99.89%. Some members of genera Acinetobacter, Actinophytocola, Aquabacterium, Dysgonomonas, Frigidibacter, Sphingobium, Serpens, and Pseudomonas dominated in crude-oil degrading microflora. Though the removal efficiency was lower than free bacteria when the temperature was 15 °C, SA and PVA+SA immobilization improved the resistance to salinity. The composite crude-oil degrading microbiota in this study demonstrated a perspective potential for crude oil removal from surface water under high salinity and low temperature conditions.

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Acknowledgements

This work was supported by the National Key R&D Program of China (No. 2018YFD0900805) and the Startup Foundation for Introducing Talent of Nanjing University of Information Science and Technology.

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Authors and Affiliations

  1. Collaborative Innovation Center of Atmospheric Environment and Equipment Technology, Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control, School of Environmental Science and Engineering, Nanjing University of Information Science and Technology, Nanjing, 210044, China

    Xiao Huang

  2. The Key Laboratory of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao, 266100, China

    Xiao Huang, Xi Chen, Jie Bai & Yangguo Zhao

  3. Harbin Institute of Technology (Shenzhen), Shenzhen Key Laboratory of Water Resource Utilization and Environmental Pollution Control, Shenzhen, 518055, China

    Ting Zhou

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  1. Xiao Huang
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  2. Ting Zhou
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Correspondence to Xi Chen.

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Huang, X., Zhou, T., Chen, X. et al. Enhanced Biodegradation of High-Salinity and Low-Temperature Crude-Oil Wastewater by Immobilized Crude-Oil Biodegrading Microbiota. J. Ocean Univ. China 21, 141–151 (2022). https://doi.org/10.1007/s11802-022-4907-4

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