Abstract
Carrageenans are widely utilized in many commercial applications such as the food and pharmaceutical industry, due to their excellent functional properties. In this study, a sensitive LC-MS/MS method was developed to determine κ-3, κ-5, and κ-7 carrageenan oligosaccharides simultaneously. Optimum MRM transitions for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were (645.079→565.111, [M-H]−), (515.137→474.946, [M-2H]2−), and (471.484→445, [M-3H]3−), respectively. Chromatographic separation was performed on an Amide column coupled with a guard column operated at 60 °C under stepwise gradient elution. The linearity of the LC-MS/MS method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides, evaluated over the concentration range of 0.10–20.0 µmol L−1, was excellent. The precisions of the method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were from 0.91% to 9.66%, and the inter-day precisions were from 0.92% to 10.5%. Validation of the LC-MS/MS method indicated that the method was precise and in line with the CFDA guidance. This method has been successfully applied to an in vitro absorption study.
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Acknowledgements
This work was supported by the Shandong Provincial Natural Science Foundation, China (No. ZR2019BC025), Open Research Fund Program of Shandong Provincial Key Laboratory of Glycoscience & Glycotechnology (Ocean University of China), and the Fundamental Research Funds for the Central Universities (Nos. 201912008, 201964019).
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Yang, Y., Zhang, P., Liu, G. et al. Simultaneous Quantification of κ-Carrageenan Oligosaccharides of DP 3, 5 and 7 by LC-MS/MS: Application to an in vitro Absorption Study. J. Ocean Univ. China 19, 1177–1182 (2020). https://doi.org/10.1007/s11802-020-4432-2
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DOI: https://doi.org/10.1007/s11802-020-4432-2