Abstract
MiR-430 is a non-coding small RNA that participates in early embryonic development by acting on the Nodal signaling pathway. Its functions in Paralichthys olivaceus (P. olivaceus), however, remain unclear. In this study, through biological information technology, we hypothesized lefty was the target gene of miR-430. Lefty is a divergent member of the transforming growth factor-β (TGF-β) superfamily, and is involved in regulating left-right patterning as an antagonist of the Nodal signaling pathway. To study the relationship between miR-430 and lefty gene, miR-430 and fluorescent expression vector were transfected into embryo cells. Our results showed that miR-430 has important effects on early embryonic development. It can inhibit the expressions of lefty and other genes related to Nodal signaling pathway. Additionally, the expression of miR-430 was involved in the metamorphosis of P. olivaceus. This study clarifies the function of miR-430 in P. olivaceus, which will be helpful for further research on the interactions between miR-430 and the Nodal signaling pathway.
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This study was supported by the National Natural Science Foundation of China (No. 31372511) and the Fundamental Research Funds for the Central Universities (No. 201822026).
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Li, R., Wang, Z., Sun, M. et al. MiR-430 Can Affect the Mesoderm Formation and Metamorphosis of Paralichthys olivaceus by Targeting lefty Gene. J. Ocean Univ. China 19, 409–416 (2020). https://doi.org/10.1007/s11802-020-4131-z
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DOI: https://doi.org/10.1007/s11802-020-4131-z