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Shallow Genetic Structure of Pholis fangi in Bohai Sea and Yellow Sea Inferred from mtDNA Control Region

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Abstract

Pholis fangi is an ecologically important fish species inhabiting Chinese coastal waters of Yellow Sea and Bohai Sea. To investigate the genetic diversity and population genetic structure of P. fangi, a fragment of 487 bp in the first hypervariable region (HVR-1) of the mitochondrial DNA (mtDNA) control region was sequenced for 181 individuals collected from Bohai Sea and Yellow Sea. A total of 18 polymorphic sites were detected, which defined 25 haplotypes. A moderate level of haplotype diversity (h = 0.7052) and a low level of nucleotide diversity (π = 0.0028) were detected. Both the phylogenetic tree and the haplotype network showed no significant genealogical structure difference among sampling locations. Pairwise FST comparison and hierarchical molecular variance analysis (AMOVA) revealed that no significant genetic structure difference existed throughout the investigated regions, suggesting a high gene exchange among different populations. The results of neutrality test and mismatch distribution analysis indicated that a late Pleistocene population expansion (38000–127000 years ago) happened. Seasonal schooling migration may contribute to the genetically homogeneous population structure of the species.

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Acknowledgements

We are grateful to Prof. Xiaoqi Zeng, Dr. Xiaodong Bian and Dr. Zhongxin Wu for collecting the samples. This study was supported by the National Natural Science Foundation of China (No. 41776171).

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Authors and Affiliations

  1. Fishery College, Zhejiang Ocean University, Zhoushan, 316022, China

    Tianxiang Gao & Hanxiang Xu

  2. Key Laboratory for Technology Research on Sustainable Utilization of Marine Fishery Resources, Zhoushan, 316022, China

    Tianxiang Gao & Hanxiang Xu

  3. Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, China

    Lin Li

  4. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Rundong Fang & Na Song

  5. Oceans and Fisheries Bureau of Dongying District, Dongying, 257091, China

    Guanghui Liu & Lei Wang

Authors
  1. Tianxiang Gao
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  2. Lin Li
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  3. Rundong Fang
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  4. Guanghui Liu
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  5. Lei Wang
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  6. Hanxiang Xu
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  7. Na Song
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Correspondence to Na Song.

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Gao, T., Li, L., Fang, R. et al. Shallow Genetic Structure of Pholis fangi in Bohai Sea and Yellow Sea Inferred from mtDNA Control Region. J. Ocean Univ. China 18, 947–952 (2019). https://doi.org/10.1007/s11802-019-3991-6

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  • DOI: https://doi.org/10.1007/s11802-019-3991-6

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