Skip to main content
SpringerLink
Log in

Subcellular Localization of Large Yellow Croaker (Larimichthys crocea) TLR21 and Expression Profiling of Its Gene in Immune Response

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

Toll-like receptor 21 (TLR21) is a non-mammalian type TLR, and plays an important role in innate immune response in fish. In this paper, the full-length cDNA sequence of TLR21 gene was identified and characterized from large yellow croaker, Larimichthys crocea and was termed as LcTLR21. It consists of 3365 bp, including a 5’-terminal untranslated region (UTR) of 97 bp, a 3’-terminal UTR of 331 bp, and an open reading frame (ORF) of 2937 bp encoding a polypeptide of 978 amino acid residues. The deduced LcTLR21 contains a signal peptide domain at N-terminal, 12 leucine-rich repeats (LRRs) at the extracellular region, a transmembrane domain and a cytoplasmic toll-interleukin-1 receptor (TIR) domain at the C-terminal. Subcellular localization analysis revealed that the LcTLR21-GFP was constitutively expressed in cytoplasm. Tissue expression analysis indicated that LcTLR21 gene broadly expressed in most of the examined tissues, with the most predominant abundance in spleen, followed by head-kidney and liver, while the weakest expression was detected in brain. The expression level of LcTLR21 after LPS, poly I:C and Vibrio parahaemolyticus challenges was investigated in spleen, head-kidney and liver. LcTLR21 gene transcripts increased significantly in all examined tissues after the challenges, and the highest expression level was detected in liver at 24 h after poly I:C stimulation (P < 0.05), suggesting that LcTLR21 might play a crucial role in fish resistance to viral and bacterial infections.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akira, S., Uematsu, S., and Takeuchi, O., 2006. Pathogen recognition and innate immunity. Cell, 124 (4): 783–801.

    Article  Google Scholar 

  • Baoprasertkul, P., Xu, P., Peatman, E., Kucuktas, H., and Liu, Z., 2007. Divergent Toll-like receptors in catfish (Ictalurus punctatus): TLR5S, TLR20, TLR21. Fish & Shellfish Immunology, 23 (6): 1218–1230.

    Article  Google Scholar 

  • Brownlie, R., Zhu, J., Allan, B., Mutwiri, G., Babiuk, L., Potter, A., and Griebel, P., 2009. Chicken TLR21 acts as a functional homologue to mammalian TLR9 in the recognition of CpG oligodeoxynucleotides. Molecular Immunology, 46 (15): 3163–3170.

    Article  Google Scholar 

  • Fan, Z., Jia, Q., and Yao, C., 2015. Characterization and expression analysis of Toll-like receptor 2 gene in large yellow croaker, Larimichthys crocea. Fish & Shellfish Immunology, 44 (1): 129–137.

    Article  Google Scholar 

  • Gao, H., Wu, L., Sun J., Geng, X., and Pan, B., 2013. Molecular characterization and expression analysis of Toll-like receptor 21 cDNA from Paralichthys olivaceus. Fish & Shellfish Immunology, 35 (4): 1138–1145.

    Article  Google Scholar 

  • Hopkins, P., and Sriskandan, S., 2005. Mammalian Toll-like receptors: To immunity and beyond. Clinical & Experimental Immunology, 140 (3): 395–407.

    Article  Google Scholar 

  • Huang, X., Wang, Z., and Yao, C., 2011. Characterization of Toll-like receptor 3 gene in large yellow croaker, Pseudosciaena crocea. Fish & Shellfish Immunology, 31 (1): 98–106.

    Article  Google Scholar 

  • Jault, C., Pichon, L., and Chluba, J., 2004. Toll-like receptor gene family and TIR-domain adapters in Danio rerio. Molecular Immunology, 40 (11): 759–771.

    Article  Google Scholar 

  • Kawai, T., and Akira, S., 2010. The role of pattern-recognition receptors in innate immunity: Update on Toll-like receptors. Nature Immunology, 11 (5): 373–384.

    Article  Google Scholar 

  • Lee, P., Zou, J., Holland, J., Martin, S., Collet, B., Kanellos, T., and Secombes, C., 2014. Identification and characterisation of TLR18-21 genes in Atlantic salmon (Salmo salar). Fish & Shellfish Immunology, 41 (2): 549–559.

    Article  Google Scholar 

  • Li, Y., Luo, X., Dan, X., Qiao, W., Huang, X., and Li, A., 2012. Molecular cloning of orange-spotted grouper (Epinephelus coioides) TLR21 and expression analysis post Cryptocaryon irritans infection. Fish & Shellfish Immunology, 32 (3): 476–481.

    Article  Google Scholar 

  • Meijer, A., Krens, S., Rodriguez I., He, S., Bitter, W., Snaar-Jagalska, B., and Spaink, H., 2004. Expression analysis of the Toll-like receptor and TIR domain adaptor families of zebrafish. Molecular Immunology, 40 (11): 773–783.

    Article  Google Scholar 

  • Oshiumi, H., Tsujita, T., Shida, K., Matsumoto, M., Ikeo, K., and Seya, T., 2003. Prediction of the prototype of the human Toll-like receptor gene family from the pufferfish, Fugu rubripes, genome. Immunogenetics, 54 (11): 791–800.

    Google Scholar 

  • Palti, Y., 2011. Toll-like receptors in bony fish: From genomics to function. Developmental & Comparative Immunology, 35 (12): 1263–1272.

    Article  Google Scholar 

  • Pietretti, D., and Wiegertjes, G., 2014. Ligand specificities of Toll-like receptors in fish: Indications from infection studies. Developmental & Comparative Immunology, 43 (2): 205–222.

    Article  Google Scholar 

  • Priyathilaka, T., Elvitigala, D., Whang, I., Lim, B., Jeong, H., Yeo, S., Choi, C., and Lee, J., 2014. Molecular characterization and transcriptional analysis of non-mammalian type Toll like receptor (TLR21) from rock bream (Oplegnathus fasciatus). Gene, 553 (2): 105–116.

    Article  Google Scholar 

  • Rauta, P., Samanta, M., Dash, H., Nayak, B., and Das, S., 2014. Toll-like receptors (TLRs) in aquatic animals: Signaling pathways, expressions and immune responses. Immunology Letters, 158 (1-2): 14–24.

    Article  Google Scholar 

  • Reyes-Becerril, M., Ascencio-Valle, F., Hirono, I., Kondo, H., Jirapongpairoj, W., Esteban, M., Alamillo, E., and Angulo, C., 2016. TLR21’s agonists in combination with Aeromonas antigens synergistically up-regulate functional TLR21 and cytokine gene expression in yellowtail leucocytes. Developmental & Comparative Immunology, 61: 107–115.

    Article  Google Scholar 

  • Saitou, N., and Nei, M., 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4 (4): 406–425.

    Google Scholar 

  • Slack, J., Schooley, K., Bonnert, T., Mitcham, J., Qwarnstrom, E., Sims, J., and Dower, S., 2000. Identification of two major sites in the type I interleukin-1 receptor cytoplasmic region responsible for coupling to pro-inflammatory signaling pathways. Journal of Biological Chemistry, 275 (7): 4670–4678.

    Article  Google Scholar 

  • Sundaram, A., Consuegra, S., Kiron, V., and Fernandes, J., 2012. Positive selection pressure within teleost Toll-like receptors tlr21 and tlr22 subfamilies and their response to temperature stress and microbial components in zebrafish. Molecular Biology Reports, 39 (9): 8965–8975.

    Article  Google Scholar 

  • Wang, W., Shen, Y., Pandit, N., and Li, J., 2013. Molecular cloning, characterization and immunological response analysis of Toll-like receptor 21 (TLR21) gene in grass carp, Ctenopharyngodon idella. Developmental & Comparative Immunology, 40 (3-4): 227–31.

    Article  Google Scholar 

  • Wang, Z., Wang, Y., Lin, L., Qiu, S., and Okamoto, N., 2001. Genetic polymorphisms in wild and cultured large yellow croaker Pseudosciaena crocea using AFLP fingerprinting. Journal of Fishery Sciences of China, 9 (3): 198–202.

    Google Scholar 

  • Yao, C., Huang, X., Fan, Z., Kong, P., and Wang, Z., 2012. Cloning and expression analysis of interferon regulatory factor (IRF) 3 and 7 in large yellow croaker, Larimichthys crocea. Fish & Shellfish Immunology, 32 (5): 869–878.

    Article  Google Scholar 

  • Yao, C., Kong, P., Wang, Z., Ji, P., Cai, M., Liu, X., and Han, X., 2008. Cloning and expression analysis of two alternative splicing Toll-like receptor 9 isoforms A and B in large yellow croaker, Pseudosciaena crocea. Fish & Shellfish Immunology, 25 (5): 648–656.

    Article  Google Scholar 

  • Yao, C., Kong, P., Wang, Z., Ji, P., Liu, X., Cai, M., and Han, X., 2009. Molecular cloning and expression of MyD88 in large yellow croaker, Pseudosciaena crocea. Fish & Shellfish Immunology, 26 (2): 249–255.

    Article  Google Scholar 

  • Yeh, D., Liu, Y., Lo, Y., Yuh, C., Yu, G., Lo, J., Luo, Y., Xiang, R., and Chuang, T., 2013. Toll-like receptor 9 and 21 have different ligand recognition profiles and cooperatively mediate activity of CpG-oligodeoxynucleotides in zebrafish. Proceedings of the National Academy of Sciences, 110 (51): 20711–20716.

    Article  Google Scholar 

  • Zhou, L., Wang, X., Liu, Q., Wang, Q., Zhao, Y., and Zhang, Y., 2010. A novel multivalent vaccine based on secretary antigendelivery induces protective immunity against Vibrio anguillarum and Aeromonas hydrophila. Journal of Biotechnology, 146 (1): 25–30.

    Article  Google Scholar 

  • Zhu, L., Nie, L., Zhu, G., Xiang, L., and Shao, J., 2013. Advances in research of fish immune-relevant genes: A comparative overview of innate and adaptive immunity in teleosts. Developmental & Comparative Immunology, 39 (1-2): 39–62.

    Article  Google Scholar 

Download references

Acknowledgements

This work was funded by the Qingdao National Laboratory for Marine Science and Technology to Cuiluan Yao, the Natural Science Foundation of Fujian (No. 2018J01454), and the National Natural Science Foundation of China (Nos. 31101882 and 41276178).

Author information

Author notes

  1. The authors contributed equally to this work.

Authors and Affiliations

  1. Fisheries College, Jimei University, Xiamen, 361021, China

    Qingxue Sun, Zejun Fan & Cuiluan Yao

Authors
  1. Qingxue Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zejun Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cuiluan Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cuiluan Yao.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, Q., Fan, Z. & Yao, C. Subcellular Localization of Large Yellow Croaker (Larimichthys crocea) TLR21 and Expression Profiling of Its Gene in Immune Response. J. Ocean Univ. China 17, 335–343 (2018). https://doi.org/10.1007/s11802-018-3361-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-018-3361-9

Keywords

Search

Navigation