Abstract
The purpose of this study was to investigate the antibacterial activity of extracts from Zostera marina against the pathogens of Apostichopus japonicus skin ulceration disease. When 95% ethanol (v/v) solvent was used to extract Zostera marina at 50°C, aqueous extract (ZA) showed obvious bacteriostatic effects on the tested bacterial strains (inhibition halo diameters between 8.23 mm and 13.62 mm), whereas the ethyl acetate extract (ZE) was almost inactive. The minimal inhibitory concentration (MIC) of ZA against four pathogens were homogeneous at 12.8 g L−1. ZA components were analyzed by thin layer chromatography (TLC) assay and six fractions were obtained. In another study, the six fractions showed inhibitory effects against the tested bacteria while their functions seemed to counteract the ZA activity.
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References
Davis, J., 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science, 264: 375–382.
Hentzer, M., Riedel, K., and Rasmussen, T. B., 2002. Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology, 148: 87–102.
Lin, X. P., Zhang, Y., and Zhang, X. Q., 2005. Antimicrobial activities of ethanol extracts from three brown algae against the phytopathogenic microorganisms and animal pathogenic bacteria. J. Fujian Normal Univ., 21(1): 112–116.
Liu, Y. G., and Wang, G. H., 2006. The effect of the Zostera marina in the rearing of Apostichopus japonicus. Shandong Fish., 23(4):12.
MA, H. Y., and Jiang, G. L., 2006. Microscopic and pathogenic research of reared Stichopus japonica suffers from a kind of skin ulceration disease. Master Thesis. Ocean University of China, Qingdao.
Murray, P. R., Baron, E. J., and Pfaller, M. A., 1995. Manual of Clinical Microbiology. Mosby Year Book, London, 78–84.
Robles, C. P. O., and Ballantine, D. L., 1996. Dynamics of antibacterial activity in three species of Caribbean marine algae as a function of habitat and life history. Hydrobiologia, 326/327: 457–462.
Robles, C. P. O., and Ballantine, D. L., 1999. Effects of culture conditions on production of antibiotically active metabolites by the marine alga Spyridea filamentosal (Ceramiaceae, Rhodophyta). J. Appl. Phycol., 11: 217–224.
Service, R. F., 1995. Antibiotics that resist resistance. Science, 270: 724–727.
Xu, N. J., and Fan, X., 2002. Study on the antibacterial activities of the ethanol of the seaweed. Oceanol. Limnol. Sin., 33(3): 265–270.
Zhang, C. Y., Wang, Y. G., and Rong, X. J., 2004. Natural resources, culture and problems of sea cucumber worldwide. Mar. Fish. Res., 25(3): 89–97.
Zhang, Y., and Jiang, G. L., 2009. Quorum sensing research of dominant microflora of Apostichopus japonica suffers from the skin ulceration syndrom. Master Thesis. Ocean University of China, Qingdao.
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Liu, Y., Jiang, G. & Wu, Z. Antibacterial activity of extracts from Zostera marina against pathogens of Apostichopus japonicus skin ulceration disease. J. Ocean Univ. China 9, 68–70 (2010). https://doi.org/10.1007/s11802-010-0068-y
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DOI: https://doi.org/10.1007/s11802-010-0068-y