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Effects of calanoid copepod Schmackeria poplesia as a live food on the growth, survival and fatty acid composition of larvae and juveniles of Japanese flounder, Paralichthys olivaceus

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Abstract

Zooplankton constitutes a major part of the diet for fish larvae in the marine food web, and it is generally believed that copepods can meet the nutritional requirements of fish larvae. In this study, calanoid copepod Schmackeria poplesia, rotifer Brachionus plicatilis and anostraca crustacean Artemia sp. were analyzed for fatty acid contents, and were used as live food for culturing larval Japanese flounder, Paralichthys olivaceus. The total content of three types of HUFAs (DHA, EPA and ARA) in S. poplesia was significantly higher than that in the other two live foods (P<0.01). Three live organisms were used for raising larvae and juveniles of Paralichthys olivaceus respectively for 15 and 10 d. Then the growth, survival and fatty acid composition of the larvae and juveniles were investigated. The results showed that the larvae and juveniles fed with copepods (S. poplesia) had significantly higher growth rate than those fed with the other two organisms (P<0.01). The survival of the flounder larvae fed with copepods was significantly higher than that of the others (P<0.01), and the survival of the juvenile fish fed with copepods was higher than that fed with Artemia (P<0.05). The contents of three types of HUFAs (DHA, EPA and ARA) and the ratio of DHA/EPA in larval and juvenile flounder P. olivaceus were analyzed. The results showed that the contents of DHA, EPA and ARA in the larvae and juveniles fed with S. poplesia were higher than those fed with a mixed diet or Artemia only, and the ratio of EPA/ARA in larvae and juveniles of P. olivaceus fed with S. poplesia was lower than that in the case of feeding with a mixed diet or Artemia only. The present data showed that copepod is the best choice for feeding the larvae and juveniles of fish considering its effects on the survival, growth and nutrition composition of the fish.

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References

  • Bell, J. G., Tocher, D. R., and Sargent, J. R., 1994. Effect of supplementation with 20:3 (n-6), 20:4 (n-6) and 20:5 (n-3) on the production of prostaglandins E and F of the 1-, 2- and 3-series in turbot (Scophthalmus maximus) brain astroglial cells in primary culture. Biochim. Biophys. Acta, 1211: 335–342.

    Google Scholar 

  • Berg, L., 1997. Commercial feasibility of semi-intensive larvi-culture of Atlantic halibut (Hippoglossus hippoglossus L.). Aquaculture, 155: 333–340.

    Article  Google Scholar 

  • Castell, J. D., Bell, J. G., Tocher D. R., and Sargent, J. R., 1994. Effects of purified diets containing different combinations of arachidonic and docosahexaenoic acid on survival, growth and fatty-acid composition of juvenile turbot (Scophthalmus maximus). Aquaculture, 128: 315–333.

    Article  Google Scholar 

  • Chu, F. E. and Dupuy, D. J., 1980. The fatty acid composition of three unicellular algal species used as food sources for larvae of the American oyster (Crassostrea virginica). Lipids, 15: 356–364.

    Article  Google Scholar 

  • Evjemo, J. O., Reitan, K. I., and Olsen Y., 2003. Copepods as live food organisms in the larval rearing of halibut larvae (Hippoglossus hippoglossus L.) with special emphasis on the nutritional value. Aquaculture, 227: 191–210.

    Article  Google Scholar 

  • Evjemo, J. O., Coutteau P., Olsen, Y., and Sorgeloos P., 1997. The stability of docosahexaenoic acid in two Artemia species following enrichment and subsequent starvation. Aquaculture, 155: 135–137.

    Article  Google Scholar 

  • Evjemo, J. O., Danielsen, T., and Olsen, Y., 2000. Losses of lipid, protein and n-3 fatty acids in enriched Artemia franciscana starved at different temperatures. Aquaculture, 193: 65–80.

    Article  Google Scholar 

  • Evjemo, J. O., and Olsen, Y., 1997. Lipid and fatty acid content in cultivated live feed organisms compared to marine copepods. Hydrobiologia, 358: 159–162.

    Article  Google Scholar 

  • Fraser, A. J., Sargent, J. R., and Gamble, J. C., 1989. Lipid class and fatty acid composition of Calanus finmarchicus (Gunnerus), Pseudocalanus sp. and Temora longicornis Muller from a nutrient-enriched seawater enclosure. J. Exp. Mar. Biol. Ecol., 130: 81–92.

    Article  Google Scholar 

  • Furuita, H., Konishi, K., and Takeuchi, T., 1999. Effects of different levels of eicosapentaenoic acid and docosahexaenoic acid in Artemia nauplii on growth, survival and salinity tolerance of larvae of the Japanese flounder, Paralichthys olivaceus. Aquaculture, 170: 59–69.

    Article  Google Scholar 

  • Furuita, H., Takeuchi, T., and Uematsu, K., 1998. Effects of eicosapentaenoic and docosahexaenoic acids on growth, survival and brain development of larval Japanese flounder (Paralichthys olivaceus). Aquaculture, 161: 269–279.

    Article  Google Scholar 

  • Furuita, H., Yamamoto, T., Shima, T., Suzuki, N., and Takeuchi, T., 2003. Effect of arachidonic acid levels in broodstock diet on larval and egg quality of Japanese flounder Paralichthys olivaceus. Aquaculture, 220: 725–735.

    Article  Google Scholar 

  • Helland, S., Terjesen, B. F., and Berg, L., 2003. Free amino acid and protein content in the planktonic copepod Temora longicornis compared to Artemia franciscana. Aquaculture, 215: 213–228.

    Article  Google Scholar 

  • Hernandez Molejon, O. G., and Alvarez-Lajonchere, L., 2003. Culture experiments with Oithona oculata Farran, 1913 (Copepoda: Cyclopoida), and its advantages as food for marine fish larvae. Aquaculture, 219: 471–483.

    Article  Google Scholar 

  • Kanazawa, A., 1985. Essential fatty acid and lipid requirement of fish. In: Nutrition and Feeding in Fish. Cowey, C. B., et al., eds., Academic Press, London, UK, 281–298.

    Google Scholar 

  • Kim, K. D., and Lee, S. M., 2004. Requirement of dietary n-3 highly unsaturated fatty acids for juvenile flounder (Paralichthys olivaceus). Aquaculture, 229: 315–323.

    Article  Google Scholar 

  • Koven, W. M., Tandler, A., Kissil, G. W., Sklan, D., Friezlander, O., and Harel, M., 1990. The effect of dietary (n-3) polyunsaturated fatty acids on growth, survival and swim bladder development in Sparus aurata. Larvae. Aquaculture, 91: 131–141.

    Article  Google Scholar 

  • Kraul, S. K., Brittain, K., Cantrell, R., Nagao, T., Ako, H., Ogasawara, A., et al., 1993. Nutritional factors affecting stress resistance in the larval mahimahi, Coryphaena hippurus. J. World Aquac. Soc., 24: 186–193.

    Article  Google Scholar 

  • Leger, P., Bengtson, D. A., Simpson, K. L., and Sorgeloos, P., 1986. The use and nutritional value of Artemia as a food source. Mar. Biol. Annu. Rev., 24: 521–623.

    Google Scholar 

  • Liu, G. X, Cui, J. L., and Huang Y., 2006. The effects of different algae on the fatty acid composition of Schmackeria poplesia (Copepoda: Calanoida). J. Xiamen Univ. (Natural Science), 45: 250–255.

    Google Scholar 

  • Lubzens, E., Marko, A., and Tietz, A., 1985. De novo synthesis of fatty acids in the rotifer, Brachionus plicatilis. Aquaculture, 47: 27–37.

    Article  Google Scholar 

  • McEvoy, L. A., Naess, T., and Lie, O., 1998. Lipid and fatty acid composition of normal and malpigmented atlantic halibut (Hippoglossus hippoglossus) fed enriched Artemia: a comparison with fry fed wild copepods. Aquaculture, 163: 237–250.

    Article  Google Scholar 

  • McEvoy, L. A., Navarro, J. C., Bell, J. G., Sargent, and J. R., 1995. Autoxidation of oil emulsions during the Artemia enrichment process. Aquaculture, 134: 101–112.

    Article  Google Scholar 

  • Naess, T., Germain-Henry, M., and Naas, K. E., 1995. First feeding of Atlantic halibut (Hippoglossus hippoglossus) using different combination of Artemia and wild zooplankton. Aquaculture, 130: 235–250.

    Article  Google Scholar 

  • Nanton, D. A., and Castell, J. D., 1998. The effects of dietary fatty acids on the fatty acid composition of the harpacticoid copepod, Tisbe sp. for use as a live food for marine fish larvae. Aquaculture, 163: 251–261.

    Article  Google Scholar 

  • Olivotto, I., Capriotti, F., Buttino, I., Avella, A. M., Vitiello, V., Maradonna, F., et al., 2008. The use of harpacticoid copepods as live prey for Amphiprion clarkii larviculture: effects on larval survival and growth. Aquaculture, 274: 347–352.

    Article  Google Scholar 

  • Olsen, Y., Reitan, K. I., and Vadstein, O., 1993. Dependence of temperature on loss rates of rotifers lipids and N3 fatty acids in starved Brachionus plicatilis cultures. Hydrobiologia, 256: 13–20.

    Article  Google Scholar 

  • Payne, M. F., and Rippingale, R, J., 2000a. Evaluation of diets for culture of the calanoid copepod Gladioferens imparipes. Aquaculture, 187: 85–96.

    Article  Google Scholar 

  • Payne, M. F., and Rippingale, R. J., 2000b. Rearing west Australian seahorse, Hippocampus subelongatus, juveniles on copepod nauplii and enriched Artemia. Aquaculture, 188: 353–361.

    Article  Google Scholar 

  • Rainuzzo, J. R., Reitan, K. I., and Olsen, Y., 1994. Effect of short and long term lipid enrichment on total lipids, lipid class and fatty acid composition in rotifers. Aquac. int., 2: 19–32.

    Article  Google Scholar 

  • Rajkumar, M., and Kumaraguru vasagam, K. P., 2006. Suitability of the copepod, Acartia clausi as a live feed for Seabass larvae (Lates calcarifer Bloch): Compared to traditional live-food organisms with special emphasis on the nutritional value. Aquaculture, 261: 649–658.

    Article  Google Scholar 

  • Sargent, J. R., Bell, J. G., Bell, M. V., Henderson, R. J., and Tocher, D. R., 1995. Requirement criteria for essential fatty acids. J. Appl. Ichthyol., 11: 183–198.

    Article  Google Scholar 

  • Sargent, J. R., 1995. Origins and functions of egg lipid. In: Broodstock Management and Egg and Larval Quality. Bromage, N. R., and Roberts, R. J., eds., Blackwell, Oxford, 353–372.

    Google Scholar 

  • Sargent, J. R., McEvoy, L. A., and Bell, J. G., 1997. Requirements, presentation and sources of polyunsaturated fatty acids in marine larval feeds. Aquaculture, 155: 117–127.

    Article  Google Scholar 

  • Schipp, G. R., Bosmans, J. M. P., and Marshall, A. J., 1999. A method for hatchery culture of tropical calanoid copepods, Acartia spp. Aquaculture, 174: 81–88.

    Article  Google Scholar 

  • Shansudin, L., Yusof, M., Azis, A., and Shukri, Y., 1997. The potential of certain indigenous copepod species as live food for commercial fish larval rearing. Aquaculture, 151: 351–356.

    Article  Google Scholar 

  • Soerensen, T. F., Drillet, G., Engell-Soerensen, K., Hansen, B. W., and Ramløv, H., 2007. Production and biochemical composition of eggs from neritic calanoid copepods reared in large outdoor tanks (Limfjord, Denmark). Aquaculture, 263: 84–96.

    Article  Google Scholar 

  • Stottrup, J. G., and Norsker N. H., 1997. Production and use of copepods in marine fish larviculture. Aquaculture, 155: 231–247.

    Article  Google Scholar 

  • Stottrup, J. G., Richardson, K., Kirkegaard, E., and Pihl N. J., 1986. The cultivation of Acartia tonsa Dana for use as live food source for marine fish larvae. Aquaculture, 52: 87–96.

    Article  Google Scholar 

  • Stottrup, J. G., Bell, J.G., and Sargent, J. R., 1999. The fate of lipids during development and cold-storage of eggs in the laboratory-reared calanoid copepod, Acartia tonsa Dana, and in response to different algal diets. Aquaculture, 176: 257–269.

    Article  Google Scholar 

  • Stottrup, J. G., and Jensen, J., 1990. Influence of algal diet on feeding and egg production of the calanoid copepod Acartia tonsa Dana. J. Exp. Mar. Biol. Ecol., 141: 87–105.

    Article  Google Scholar 

  • Toledo, J. D., Golez Ma, S., Doi, M., Ohno, A., 1999. Use of copepod nauplii during early feeding stage of grouper Epinephalus coioides. Fish. Sci., 65: 390–397.

    Google Scholar 

  • Volkman, J. K., Jeffrey, S. W., Nichols, P. D., Rogers, G. I., and Garland, C. D., 1989. Fatty acid and lipid composition of l0 species of microalgae used in mariculture. J. Exp. Mar. Biol. Ecol., 128: 2l9–240.

    Article  Google Scholar 

  • Watanabe, T., Arakawa, T., Takeuchi, T., and Satoh, S., 1989. Comparison between eicosapentaenoic and docosahexaenoic acids in terms of essential fatty acid efficiency in juvenile striped jack Pseudocaranx dentex. Nippon Suisan Gakkaishi, 55: 1989–1995.

    Google Scholar 

  • Watanabe, T., Kitajima C., and Fujita S., 1983. Nutritional profiles of live organisms used in Japan for mass propagation of fish: a review. Aquaculture, 34: 115–143.

    Article  Google Scholar 

  • Watanabe, T., 1993. Importance of docosahexaenoic acid in marine larval fish. J. World Aquac. Soc., 24: 152–161.

    Article  Google Scholar 

  • Watanabe, T., and Kiron, V., 1994. Prospects in larval dietetics. Aquaculture, 124: 223–251.

    Article  Google Scholar 

  • Witt, U., Quantz, G., Kuhlmann, D., and Kattner, G., 1984. Survival and growth of turbot larvae Scophthalmus maximus L. reared on different food organisms with special regard to long-chain polyunsaturated fatty acids. Aquac. Eng., 3: 177–190.

    Article  Google Scholar 

  • Woods, C. M. C., 2003. Growth and survival of juvenile seahorse Hippocampus abdominalis reared on live, frozen and artificial foods. Aquaculture, 220: 287–298.

    Article  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Marine Environmental Science and Ecology, Ministry of Education, Ocean University of China, Qingdao, 266100, P. R. China

    Guangxing Liu

  2. College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, P. R. China

    Guangxing Liu & Donghui Xu

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  1. Guangxing Liu
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Correspondence to Guangxing Liu.

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Liu, G., Xu, D. Effects of calanoid copepod Schmackeria poplesia as a live food on the growth, survival and fatty acid composition of larvae and juveniles of Japanese flounder, Paralichthys olivaceus . J. Ocean Univ. China 8, 359–365 (2009). https://doi.org/10.1007/s11802-009-0359-3

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