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Performance of a high-rate anammox reactor under high hydraulic loadings: Physicochemical properties, microbial structure and process kinetics

高负荷厌氧氨氧化反应器性能、微生物结构和过程动力学特性研究

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Abstract

In this study, a lab-scale upflow anaerobic sludge blanket (UASB) reactor was applied to studying the high-rate nitrogen removal of granule-based anammox process. The nitrogen removal rate (NRR) finally improved to 15.77 kg/m3/d by shortening hydraulic retention time (HRT) to 1.06 h. Well-shaped red anammox granules were extensively enriched inside the reactor. The results of nitrogen removal kinetics indicated that the present bioreactor has great nitrogen removal potential, because the maximum rate of substrate utilization (Umax) predicted by Stover-Kincannon model is suggested as 55.68 kg/(m3·d). Analysis of the microbial community showed that the anammox genus Candidatus Kuenenia dominated the bacterial communities. The relative abundance of Candidatus Kuenenia rose from 12.29% to 36.95% after progressively shorter HRT and higher influent substrate concentrations, illustrating the stability of nitrogen removal performance and biomass enrichment offered by the UASB in carrying out high-rate anammox process.

摘要

本文研究了上流式厌氧污泥床(UASB)中厌氧氨氧化工艺的脱氮性能、污泥性状、微生物群落 结构以及过程动力学特性。首先, 通过缩短水力停留时间并提高进水基质浓度, 研究了负荷提升过程 中UASB 反应器的脱氮性能, 并最终将反应器的总氮去除速率(NRR)提高到15.77 kg/(m3·d), 富集了 成熟的厌氧氨氧化颗粒污泥。其次, 选用3 种基质去除动力学模型对反应器稳态运行时期的数据进行 了拟合, 其中Stover-Kincannon 模型的结果表明UASB 的最大基质利用速率(Umax)可达55.68 kg/(m3·d)。 此外, 微生物群落结构分析的结果表明反应器在长期高负荷运行后, 占主导地位的脱氮细菌为 “Candidatus Kuenenia”属。随着脱氮性能的提高, 其相对丰度由12.29%提高到了36.95%, 颗粒污泥内 的厌氧氨氧化优势菌属的组成也发生了改变。

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Authors and Affiliations

  1. College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China

    Yu-xia Song  (宋雨夏)

  2. School of Metallurgy and Environment, Central South University, Changsha, 410083, China

    Yu-xia Song  (宋雨夏), M. Ali, Fan Feng  (冯帆), Yun-yan Wang  (王云燕) & Chong-jian Tang  (唐崇俭)

  3. National Engineering Research Centre for Control and Treatment of Heavy Metal Pollution, Changsha, 410083, China

    Yu-xia Song  (宋雨夏), M. Ali, Fan Feng  (冯帆), Xi-lin Chai  (柴喜林), Yun-yan Wang  (王云燕) & Chong-jian Tang  (唐崇俭)

  4. Jiangsu Key Laboratory of Anaerobic Biotechnology, Jiangnan University, Wuxi, 214122, China

    Shuo Wang  (王硕)

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  1. Yu-xia Song  (宋雨夏)
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Correspondence to Xi-lin Chai  (柴喜林) or Chong-jian Tang  (唐崇俭).

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Foundation item: Project(51878662) supported by the National Natural Science Foundation of China; Project(2017SK2420) supported by the Science and Technology of Hunan Province, China; Project(2019JJ20033) supported by the Distinguished Youth Natural Science Foundation of Hunan Province, China

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Song, Yx., Ali, M., Feng, F. et al. Performance of a high-rate anammox reactor under high hydraulic loadings: Physicochemical properties, microbial structure and process kinetics. J. Cent. South Univ. 27, 1197–1210 (2020). https://doi.org/10.1007/s11771-020-4360-8

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