Abstract
To investigate the feasibility of implanting the biocomposite of calcium phosphate cement (CPC)/polylactic acid-polyglycolic acid (PLGA) into animals for bone defects repairing, the biocomposite of CPC/PLGA was prepared and its setting time, compressive strength, elastic modulus, pH values, phase composition of the samples, degradability and biocompatibility in vitro were tested. The above-mentioned composite implanted with bone marrow stromal cells was used to repair defects of the radius in rabbits. Osteogenesis was histomorphologically observed by using an electron-microscope. The results show that compared with the CPC, the physical and chemical properties of CPC/PLGA composite have some differences in which CPC/PLGA composite has better biological properties. The CPC/PLGA composite combined with seed cells is superior to the control in terms of the amount of new bones formed after CPC/PLGA composite is implanted into the rabbits, as well as the speed of repairing bone defects. The results suggest that the constructed CPC/PLGA composite basically meets the requirements of tissue engineering scaffold materials and that the CPC/PLGA composite implanted with bone marrow stromal cells may be a new artificial bone material for repairing bone defects because it can promote the growth of bone tissues.
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References
CRANE G M, ISHAUG S L, MIKOS A G. Bone tissue engineering [J]. Nat Med, 1995, 1(12): 1322–1324.
ZHENG Lei, WANG Qian, PEI Guo-xian. Selection on extracellular matrix materials in bone tissue engineering [J]. Chinese Journal of Surgery, 2000, 38(10): 745–748.
BAI Bing, YE Mao-chang. The current situation and future of extracellular matrix materials in bone tissue engineering [J]. Progress in Modern Biomedicine, 2007, 7(1): 138–141. (in Chinese)
ZHANG Xin-feng, ZHANG Zhi-hong. Research development of mineralized biomaterials with osteoinductivity [J]. Progress in Modern Biomedicine, 2007, 7(5): 777–779. (in Chinese)
DRIESSENS F C M, BOLTONG M G, BERMUDEZ O, PLANELL J A. Formulation and setting times of some calcium orthophosphate cements: A pilot study [J]. J Mater Sci: Mater in Med, 1993, 4(5): 503–508.
BAADSGAARD K. Defect pseudoarthroses: An experimental study in rabbits [J]. Acta Orthop Scand, 1969, 40(6): 689–695.
ZELLIN G, LINDE A. Treatment of segmental defects in long bones using osteopromotive membranes and recombinant human bone morphogenetic protein-2: An experimental study in rabbits [J]. Scand J Plast Reconstr Surg Hand Surg, 1997, 31(2): 97–104.
FEI Zheng-qi, HU Yun-yu, WU Dao-chen, WU Hong, LU Rong, BAI Jian-ping, SONG Hong-xun. Preparation and property of a novel bone graft composite consisting of rhBMP-2 loaded PLGA microspheres and calcium. Phosphate cement [J]. Journal of Materials Science: Material in Medicine, 2007, 19(3): 1109–1116.
LI Qi-hong. Progress in research of repairing large segmental bone defects of long bones in China [J]. Chinese Journal of Orthopaedics, 1997, 17: 13–14. (in Chinese)
LSHIKAWA K, ASAOKA K. Estimation of ideal mechanical strength and critical porosity of calcium phosphate cement [J]. J Biomed Mater Res, 1995, 29(12): 1537–1543.
GINEBRA M P, FERNANDEZ E, BOLTONG M G, BERMUDEZ O, PLANELL J A, DRIESSENS F C M. Compliance of an apatitic calcium phosphate cement with the short-term clinical requirements in bone surgery, orthopaedics and dentistry [J]. Clin Mater, 1994, 17(2): 99–104.
XU Jin, XIONG Gui-rong, JIANG Ping. Relationships between stress distribution of mandible with age and incidence of fracture [J]. Journal of Jiangsu University: Medicine Edition, 2006, 16(4): 291–293. (in Chinese)
ZHANG Wei. The preparation of, and experimental study on compound bioactive artificial bone (CBAB) [D]. Shanghai: The Second Military Medical University, 2001. (in Chinese)
MURUGAN R, RAMAKRISHNA S. Nano-featured scaffolds for tissue engineering: A review of spinning methodologles [J]. Tissue Engineering, 2006, 12(3): 435–447.
XIANG Qi-jun, LIU Yong, ZHENG Zhi, SU Zhe-an, WANG Jian-long, XIAO Fei. Preparation and some property investigations on composite of calcium phosphate cements/polyglycolic-polylactic acid [J]. Materials Science and Engineering of Powder Metallurgy, 2005, 10(6): 361–364. (in Chinese)
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Foundation item: Projects(30370412, 30670558) supported by the National Natural Science Foundation of China
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Zheng, Z., Wang, Jl., Mi, L. et al. Preparation of new tissue engineering bone-CPC/PLGA composite and its application in animal bone defects. J. Cent. South Univ. Technol. 17, 202–210 (2010). https://doi.org/10.1007/s11771-010-0031-5
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DOI: https://doi.org/10.1007/s11771-010-0031-5