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Novel fluorescence sensor based on covalent immobilization of 3-amino-9-ethylcarbazole via electrostatically assembled gold nanoparticle layer

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Abstract

A novel technique of immobilizing indicator dyes by electrostatic adsorption and covalent bonding to fabricate optical sensors was developed. 3-Amino-9-ethylcarbazole (AEC) was attached to the outmost surface of quartz glass slide via aminosilanizing the slide, crosslinking chitosan, adsorbing Au nanoparticle, self-assembling HS(CH2)11OH, and coupling AEC. Thus, an AEC-immobilized optical sensor was obtained. The sensor exhibits a wide linear response range from 7.0×10−7 to 1.0×10−4 mol/L and a correlation coefficient of 0.995 9 for the detection of 2-nitrophenol. The detection limit and response time of the sensor are 1.0×10−7 mol/L and less than 10 s, respectively. The fluorescence intensity of the used sensor can be restored to the blank value by simply rinsing with blank buffer. A very effective matrix for immobilizing indicator dye is provided by the proposed technique, which is adaptable to other indicator dyes with amino groups besides AEC.

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Authors and Affiliations

  1. School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, China

    Shu-zhen Tan  (谭淑珍), Shu Long  (龙 姝), Jiao-yun Xia  (夏姣云), Zhong Cao  (曹 忠), Ling Zhang  (张 玲) & Fu-chun Gong  (龚福春)

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  1. Shu-zhen Tan  (谭淑珍)
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  2. Shu Long  (龙 姝)
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  3. Jiao-yun Xia  (夏姣云)
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  4. Zhong Cao  (曹 忠)
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  5. Ling Zhang  (张 玲)
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  6. Fu-chun Gong  (龚福春)
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Correspondence to Shu-zhen Tan  (谭淑珍).

Additional information

Foundation item: Project(20775010) supported by the National Natural Science Foundation of China; Project(208095) supported by the Key Project of Ministry of Education, China; Project(07A006) supported by the Scientific Research Fund of Hunan Provincial Education Department, China; Project(07JJ3020) supported by Hunan Provincial Natural Science Foundation of China

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Tan, Sz., Long, S., Xia, Jy. et al. Novel fluorescence sensor based on covalent immobilization of 3-amino-9-ethylcarbazole via electrostatically assembled gold nanoparticle layer. J. Cent. South Univ. Technol. 16, 212–217 (2009). https://doi.org/10.1007/s11771-009-0036-0

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  • DOI: https://doi.org/10.1007/s11771-009-0036-0

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