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Extraction optimization of coumarins from radix angelicae pubescentis by HPLC-DAD coupled with uniform design

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Abstract

Uniform design was used to optimize extraction condition of direct refluence extraction of coumarins from the Chinese traditional medicine of radix angelicae pubescentis(Duhuo); the sum peak area of coumarins separated with high performance liquid chromatography (HPLC) at detection wavelength of 320 nm was considered as detection index, two factors of solvent concentration and extraction time were mainly studied at extraction temperature of 85°C and a volume ratio of solvent to sample of 10 : 1. Optimal subclass, quadric polynomial step by step aggression and neural network method were applied to process the experimental results. The results show that the first and second methods give the same factors combination (concentration of ethanol: 95%, extraction time: 3.6 h) and the second method is much better than the first one. The extraction model is consequently developed.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Central South University, 410083, Changsha, China

    Guo Fang-qiu PhD (Associate professor), Huang Lan-fang, Zhou Huo-fei & Jiang Qiu-ju

  2. Technical Center of Changde Cigarette Factory, 415000, Changde, China

    Huang Lan-fang

Authors
  1. Guo Fang-qiu PhD
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  2. Huang Lan-fang
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  3. Zhou Huo-fei
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  4. Jiang Qiu-ju
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Correspondence to Guo Fang-qiu PhD.

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Foundation item: Project(2004714) supported by the Development and Innovation Committee of Hunan Province

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Guo, Fq., Huang, Lf., Zhou, Hf. et al. Extraction optimization of coumarins from radix angelicae pubescentis by HPLC-DAD coupled with uniform design. J Cent. South Univ. Technol. 13, 156–159 (2006). https://doi.org/10.1007/s11771-006-0148-8

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  • DOI: https://doi.org/10.1007/s11771-006-0148-8

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