Abstract
An accelerated solvent extraction (ASE) method was investigated to maximize the total phenolic content (TPC) and the antioxidant activity (DPPH) of chaga extracts as measured by response surface methodology (RSM). The effectiveness of ASE as a green extraction technique was compared to conventional methods based on TPC and DPPH analyses. High-performance liquid chromatography (HPLC) was used to assess the effects of ASE extraction temperatures on individual phenolic compound quality and yield. Results indicate that an extraction temperature of 170 °C and ethanol concentration of 66% are optimal for recovery of phenolic compounds. The TPC and DPPH in the ASE extracts were greater than in those extracted with conventional methods suggesting ASE is a viable green alternative for extracting phenolics from chaga. With the exception of caffeic acid, a significant increase in the yield of all the assayed phenolic acids was observed with an increased extraction temperature up to 180 °C.
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Data Availability
The datasets generated and/or analyzed in this study are available from the corresponding author on reasonable request.
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Acknowledgements
Special thanks are extended to Joan H. Perkins for editing the manuscript.
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This research was financially supported by the USDA NIFA (United States) Hatch No: ME021817 and the Maine Agricultural Experiment Station.
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Weaam Alhallaf declares that she has no conflict of interest. Karl Bishop declares that she has no conflict of interest. L. Brian Perkins declares that he has no conflict of interest.
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Alhallaf, W., Bishop, K. & Perkins, L.B. Optimization of Accelerated Solvent Extraction of Phenolic Compounds from Chaga Using Response Surface Methodology. Food Anal. Methods 15, 2777–2790 (2022). https://doi.org/10.1007/s12161-022-02319-x
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DOI: https://doi.org/10.1007/s12161-022-02319-x