Abstract
The strain Lv1–2 isolated from the Henan bauxite was characterized by morphological observation, biochemical and physiological identification, and 16S rDNA sequence analysis. The influences of temperature, initial pH value, the volume of medium, shaking speed and illite concentration on the desilicating ability of the strain Lv1–2 were investigated. The results show that the bacterium is a Gram-negative rod-shaped bacterium with oval endspores and thick capsule, but without flagellum. The biochemical and physiological tests indicate that the strain Lv1–2 is similar to Bacillus mucilaginosus. In GenBank the 16S rDNA sequence similarity of the strain Lv1–2 and the B. mucilaginosus YNUCC0001 (AY571332) is more than 99%. Based on the above results, the strain Lv1–2 is identified as B. mucilaginosus. The optimum conditions for the strain Lv1–2 to remove silicon from illite are as follows: temperature is 30 °C; initial pH value is 7.5; medium volume in 200 mL bottle is 60 mL; shaking speed of rotary shaker is 220 r/m; illite concentration is 1%.
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JIANG Hao, LI Guang-hui, HU Yue-hua. The separation of aluminium and silicon in bauxite[J]. Metallic Ore Dressing Abroad, 2001, 38(5): 24–34. (in Chinese)
LI Guang-hui, JIANG Tao, FAN Xiao-hui. The thermochemistry activation of silicate in illite and desilicating [J]. Metal Mine, 2004, 337(7): 18–21. (in Chinese)
Yakhontova L K, Andrevev P I, Sidyakina G G, et al. Breakdown of nickel silicates and berthierine by silicate bacteria [J]. Transactions of the USSR Academy of Sciences: Earth Science Sections, 1989, 309(6): 165–167.
HE Lin-yan, YIN Yong-xian, HUANG Wei-yi. The identification of a slicate bacteria and the analysis of its phylogenesis[J]. Acta Microbiologica Sinica: 2003, 43(2): 162–168. (in Chinese)
ZHOU Gi-kui, HU Yue-hua, QIU Guan-zhou. Advances in the application research of slicate bacteria in mineral engineering field [J]. Metal Mine, 2002, 307(1): 26–28. (in Chinese)
Willscher S, Bosecker K. Studies on the leaching behaviour of heterotrophic microorganisms isolated from an alkaline slag dump [J]. Hydrometallurgy, 2003, 71(10): 257–264.
Yakhontova L K, Nesterovich L G, Sidyakina G G. Possibility of bacterial leaching of aluminum from dawsonite[J]. Earth Science Section, 1994, 322(1): 152–156.
Styriakoca I, Styriak I, Kraus I, et al. Biodestruction and deferritization of quartz sands by bacillus species [J]. Minerals Engineering, 2003, 16(8): 709–713.
Styriakova I, Styriak I. Iron removal from kaolins by bacterial leaching [J]. Ceramics-Silikaty, 2000, 44(11): 135–141.
Styriakova I, Styriak I, Gslko I, et al. The release of iron-bearing minerals and dissolution of feldspars by heretotrophic bacteria of bacillus species[J]. Ceramics-Silikaty, 2003, 47 (1): 20–26.
HE Ji-qiang, LI Deng-yu, ZHANG Xiao-ping, et al. Phenotypic aspects and phosphorus-releasing and potassium-releasing ability of silicate bacteria isolated from purple soils [J]. Chin J Appl Environ Biol, 2003, 9(1): 71–77. (in Chinese)
SHENG Xia-fang, HUANG Wei-yi, CAO Xiao-ying. Dissolution of feldspar and potassium uptake by the strain NBT of silicate bacterium [J]. Plant Nutrition and Fertilizer Science, 2001, 7(4): 459–466. (in Chinese)
WANG Kang-lin, HAN Xiao-zhao, et al. Study on screening of silicate bacteria and potassium extraction [J]. Chemical Engineering Mineral and Process, 2005, (2): 25–27. (in Chinese)
NIU Yin-jian, QIU Guan-zhou, ZhOU Ji-kui. Screening of silicate bacteria and bioleaching silicon from bauxite[J]. The Chinese Journal of Nonferrous Metals, 2004, 14(2): 280–285. (in Chinese)
LIAN Bin. The effect of slicate bacteria to release potassium from illite [J]. Acta Mineralogica Sinica, 1998, 18(2): 234–238. (in Chinese)
Editorial Board of Environment Protection Bureau of China. Monitoring and determination methods for water and wastewater [M]. 3th ed. Beijing: China Environmental Science Press, 1998. (in Chinese).
DONG Xiu-zhu, CAI Miao-ying. Manual of common determinative bacteriology [M]. Beijing: Science Press, 2001. (in Chinese)
Joseph S, David W R. Molecular cloning: A laboratory press [M]. Beijing: Science Press, 2003. (in Chinese)
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Foundation item: Project (50321402) supported by the National Natural Science Foundation of China; project (2004CB619204) supported by the National Basic Research Program
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Zhou, Hb., Zeng, Xx., Liu, Ff. et al. Screening, identification and desilication of a silicate bacterium. J Cent. South Univ. Technol. 13, 337–341 (2006). https://doi.org/10.1007/s11771-006-0045-1
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DOI: https://doi.org/10.1007/s11771-006-0045-1