Abstract
The time course of xylo-oligosaccharides concentration and xylo-oligosaccharides yield in the separation of xylo-oligosaccharides from enzymatic hydrolytes was studied using a membrane reactor with constant permeate flux of 4 L · m−2 · h−1. The results show that xylanases retain 90% of its activity in the reactor. The concentration of xylo-oligosaccharides achieves the maximum, about 5.48 g/L in 30 min. The difference of xylo-oligosaccharides in the retentate and permeate stream is low, <0.62 g/L, therefore it can permeate through membrane. Under the operating conditions that xylan concentration is 30.0 g/L, pH 5.0, operating pressure 16 kPa, temperature 48 °C, feed velocity 400 mL/min, reaction volume 400 mL, enzyme dosage 10% (volume fraction), dilution rate 1 h−1, and enzymatic hydrolysis time 195 min, the yield of xylo-oligosaccharides reaches 31.69%.
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Foundation item: The National Natural Science Foundation of China(No. 30070636); The Natural Science Foundation of the Education Department of Anhui Province(No. 2002kj 117)
Biography of the first author: YANG Fu-guo, doctoral student, associate professor, born in 1964, majoring in membrane separation technology.
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Yang, Fg., Fang, Z., Xu, Y. et al. Separation of xylo-oligosaccharides from enzymatic hydrolytes using membrane reactor. J Cent. South Univ. Technol. 10, 122–125 (2003). https://doi.org/10.1007/s11771-003-0052-4
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DOI: https://doi.org/10.1007/s11771-003-0052-4