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Comparative morphometrics, molecular characterization, and dynamic population of Zeldia punctata associated with mustard crops from India

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Abstract

A survey was conducted in the agricultural fields of mustard of Haridwar district, Uttarakhand, India, where a heavy population of Zeldia was recovered. The morphological, molecular, and phylogenetic characterization confirmed the nematode as Zeldia punctata. The phylogenetic analysis based on 18 and 28S rDNA placed the studied population of Z. punctata close to other populations of the same species. The principal component analysis (PCA) data revealed the high intraspecific and interspecific variations between the populations of Zeldia punctata from different geographical regions. The result indicated a variation between the Indian populations of Z. punctata. Additionally, the selected ecological factors indicated an impact of EC, temperature, and phosphorus on Z. punctata during the flowering and podding time of mustard crops in India. However, the PCA revealed no effect of ecological factors on the nematode diversity indices. In conclusion, Z. punctata, with its high individual numbers on mustard, may affect crop production due to activating the bacterial community of the soil and less space for plant-parasitic nematodes.

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The data and material of this manuscript are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank the Department of Zoology, Chaudhary Charan Singh University, Meerut, for providing the necessary laboratory facilities for conducting the experiments. Head SAIF AIIMS, New Delhi, is also being acknowledged for providing scanning electron microscopic facilities.

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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Nematology Laboratory, Department of Zoology, Chaudhary Charan Singh University, 250004, Meerut, India

    Himani Sharma, Lovely Bharti & Ashok Kumar Chaubey

  2. Department of Research Administration and Development, University of Limpopo, Private Bag X1106, 0727, Sovenga, South Africa

    Ebrahim Shokoohi

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  1. Himani Sharma
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  4. Ebrahim Shokoohi
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Contributions

The study was designed by HS and AKC. Interpretation of the data was done by HS and ES. Manuscript writing was done by HS. Tables and reference settings were done by LB and HS. Errors and grammatical mistakes in the manuscript were removed and edited by AKC and ES. Then, the final manuscript was read and approved by all authors.

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Correspondence to Himani Sharma.

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Sharma, H., Bharti, L., Chaubey, A.K. et al. Comparative morphometrics, molecular characterization, and dynamic population of Zeldia punctata associated with mustard crops from India. Biologia (2024). https://doi.org/10.1007/s11756-024-01658-w

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