Abstract
The current manuscript focuses on the isolation of a potent lipase-producing mesophilic bacteria and explores the optimization of culture parameters to enhance lipase production. From a total of 83 bacterial isolates, after screening on tributyrin agar plate, rhodamine olive oil agar plate, and submerged fermentation studies, the isolate VT 1 with a lipase activity of 23.85 U/mL was selected. The potent isolate VT 1 was identified as Serratia marcescens (accession number OM757842) based on 16S rRNA gene sequencing and standard morphological and biochemical procedures. The lipase production was optimized by a statistical approach using response surface methodology after identifying the significant factors with Plackett-Burman design using Design-Expert, version 11 software. Nutrient factors- peptone, yeast extract, sodium chloride, and olive oil- were identified as the significant factors. Its optimization via central composite design of response surface methodology with Design-Expert, version 11 (olive oil: 50 mL/L, sodium chloride: 8.87 g/L, peptone: 8.15 g/L, yeast extract: 10 g/L) resulted in an enhanced experimental lipase activity of 55.95 U/mL in comparison to the predicted lipase activity of 56.858 U/mL. The study brought forth the isolation of a lipase-producing Serratia marcescens strain VT 1 and highlighted the importance of optimizing culture parameters for augmented production of lipase. The optimization via response surface methodology resulted in a 2.34-fold increase in lipase production.
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Data availability
The data analyzed during the study will be made available on reasonable request from the corresponding author.
Abbreviations
- ANOVA:
-
Analysis of variance
- BBD:
-
Box-Behnken Design
- BLASTN:
-
Basic Local Alignment Search Tool for Nucleotide sequences
- CCD:
-
Central Composite Design
- DOE:
-
Design of Experiments
- MALDI-TOF MS:
-
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
- MEGA11:
-
Molecular Evolutionary Genetics Analysis version 11
- MTCC:
-
Microbial Type Culture Collection and Gene Bank
- NCBI:
-
National Center for Biotechnology Information
- OFAT/OVAT:
-
One Factor/Variable at a Time
- PCR:
-
Polymerase Chain Reaction
- PBD:
-
Plackett-Burman Design
- PNPP:
-
4-Nitrophenyl palmitate/p-nitrophenyl palmitate
- PRESS:
-
Predicted Residual Sum of Squares
- rRNA:
-
Ribosomal ribonucleic acid
- ROA:
-
Rhodamine Olive Oil Agar
- RSM:
-
Response Surface Methodology
- SMVT 1:
-
Serratia marcescens VT 1
- TBA:
-
Tributyrin Agar
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Acknowledgements
The author thanks the Principal and Department of Botany, University College, Palayam, Thiruvananthapuram, for providing laboratory facilities. The author is grateful to Dr. Subramaniyan S, Principal and Professor, Government Arts College, Thiruvananthapuram, and Dr. Sandhia G.S, Principal and Professor, Government College, Kariavattom, Thiruvananthapuram, for all their guidance and support during the period of work. The author is thankful to the Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, and Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Chandigarh, India, for the identification of microorganisms. The author is also thankful to the University of Kerala, Thiruvananthapuram, for the fund provided during work.
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The present work was funded by the University of Kerala, Palayam, Thiruvananthapuram.
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Vivek K conceptualized the study, collected the literature and methodology, performed the experiments, wrote, and edited the manuscript.
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Krishnankutty, V. Optimization of lipase production by response surface methodology from Serratia marcescens VT 1 isolated from oil contaminated soil. Biologia 79, 1471–1486 (2024). https://doi.org/10.1007/s11756-024-01614-8
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DOI: https://doi.org/10.1007/s11756-024-01614-8