Abstract
Although mushroom production is sterile, bacterial communities are associated with the mushroom culture process. After noticing colonies of bacteria on the mycelium, spawn, and culture surfaces, we aim to isolate and identify the bacterial species present during each stage of Pleurotus eryngii culture. For this purpose, bacteria were characterized biochemically by catalase and oxidase assays and molecularly by 16S ribosomal RNA sequencing and were found to belong to the families of Enterobacteriaceae and Bacillaceae. Results reveal the presence of five species: Bacillus subtilis, B. tequilensis, B. cereus, Ewingella americana, and Klebsiella grimontii. The inhibitory activity of the isolated bacteria on mycelia growth on PDA reveals that Klebsiella grimonti and Bacillus cereus have a significant inhibitory effect on the development of Pleurotus eryngii var. ferulae mycelium.
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All data was included in the manuscript.
Abbreviations
- 16S rRNA:
-
16S ribosomal RNA
- ANOVA:
-
Analysis of Variance
- BET:
-
Ethidium bromide
- BHI:
-
Solid Brain Heart Infusion
- BLAST:
-
Basic Local Alignment Search Tool
- BLAST:
-
Basic local alignment search tool
- Bp:
-
Base pair
- CaCO3 :
-
Calcium carbonate
- ddNTPs:
-
Dideoxynucleotide triphosphates
- DNA:
-
Deoxyribonucleic acid
- dNTPs:
-
Deoxynucleotide triphosphates
- PCR:
-
Polymerase chain reaction
- PDA:
-
Potato Dextrose Agar
- Rpm:
-
Rotation per minute
- rRNA:
-
Ribosomal ribonucleic acid
- SAP:
-
Shrimp alkaline phosphatase
- TAE:
-
Tris Acetate EDTA
- TE:
-
Tris- EDTA
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Acknowledgements
We thank the Plant, Soil and Environment Interactions Laboratory at the University of El-Manar, Faculty of Sciences and the Epidemiology Laboratory and Veterinary Microbiology, Bacteriology and Biotechnology Development Group at the Pasteur Institute of Tunis for providing lab facilities.
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Jaffali, C., Khadhri, A., Kmiha, S. et al. A preliminary overview of possible bacterial communities interacting with Pleurotus eryngii cultivation process. Biologia 78, 2649–2656 (2023). https://doi.org/10.1007/s11756-023-01420-8
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DOI: https://doi.org/10.1007/s11756-023-01420-8