Abstract
Wheat production, productivity, and quality has been severely affected by drought and heat stresses across the world. Glaucousness, a light bluish appearance on flag leaf, stem, and spikes, is one of the adaptive traits significantly contributing towards tolerance to heat and drought stresses. In this study, a set of 100 genotypes were screened under irrigated (IR), restricted irrigation (RI), and late sown environments (LS) for morpho-physiological and agronomic traits to find their association with glaucousness. Glaucousness exhibited a progressive increase under stressed conditions. Glaucousness had positive correlation with yield and negative correlation with normalized difference vegetation index (NDVI) in all three environments suggesting its confounding effects on NDVI trait. Further, molecular characterization at W1 gene cluster with linked markers indicated no polymorphism in the glaucous and glossy genotypes. However, expression analysis of five genotypes for these genes showed higher abundance in glaucous and reduced abundance in the non-glaucous line under stress. Marker-trait association analysis using 35K SNP array revealed a significant association of glaucousness with AX-94844376 on 2D chromosome. The information generated on associations between various traits may enhance our potential for the selection of tolerant genotypes and will also improve our understanding of glaucousness at the molecular level.
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Abbreviations
- NDVI:
-
Normalized difference vegetation index.
- SNP:
-
Single nucleotide polymorphism.
- TGW:
-
Thousand grain weight.
- DH:
-
Days to 50% heading.
- GL:
-
Grain length.
- GWD:
-
Grain width.
- GLN:
-
Glaucousness.
- SC:
-
Stomatal conductance.
- CT:
-
Canopy temperature.
- HSI:
-
Heat susceptibility index.
- DSI:
-
Drought susceptibility index.
- BLUPs:
-
Best linear unbiased predictions.
- ANOVA:
-
Analysis of variance.
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Thippeswamy, H., Krishna, H., Sinha, N. et al. Assessing the role of glaucousness in imparting tolerance to moisture and heat stress in wheat. Biologia 77, 3279–3289 (2022). https://doi.org/10.1007/s11756-022-01177-6
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DOI: https://doi.org/10.1007/s11756-022-01177-6