Abstract
Pneumonia is a clinical condition that can increase oxidative stress and can cause hospitalization and intensive care unit (ICU) stay. Oxidative stress increases the production of pulmonary inflammatory mediators in the respiratory system, and this inflammatory process can cause DNA damage. This study aimed to determine the DNA damage levels of patients diagnosed with pneumonia and monitored in the ICU. From 50 patients and 30 healthy volunteers, 3 mL of blood was drawn, and DNA damage was determined using the Comet assay. Two slides were prepared for each sample, and 50 randomly selected cells from each slide were photographed using Zeiss Imager A1 fluorescence microscope and Axiocam Icc 1 camera. The photographs were analyzed using the OpenComet software and tail DNA percentage parameter was chosen to demonstrate DNA damage. The DNA damage of intubated, non-intubated, and all pneumonia patients were compared with the control group. DNA damage was 2.48 ± 2.81 in the control group, 3.19 ± 5.99 in the pneumonia group, 2.97 ± 5.58 in intubated patients, and 3.50 ± 6.32 in non-intubated patients. DNA damage was higher in all groups than in the control group (p < 0.001). However, the DNA damage of intubated patients was significantly lower than that of non-intubated patients (p < 0.001). As a result of the study, it was found that DNA damage decreased in patients who were intubated and received oxygen support.
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Abbreviations
- °C:
-
Celsius.
- µL:
-
Microliter.
- cm:
-
Centimeter.
- DAMPs:
-
Damage-associated molecular patterns.
- DMSO:
-
Dimethyl sulfoxide.
- DNA:
-
Deoxyribonucleic acid.
- FIO2 :
-
Fraction of inspired oxygen.
- H2O:
-
Water.
- Hg:
-
Mercury.
- ICU:
-
Intensive care unit.
- LMA:
-
Low melting point agarose.
- M:
-
Molar.
- Min:
-
Minute.
- mL:
-
Milliliter.
- mM:
-
Micromolar.
- mm:
-
Millimeter.
- mtDNA:
-
Mitochondrial Deoxyribonucleic acid.
- Na2-EDTA:
-
Ethylenediaminetetraacetic acid disodium salt solution.
- NaCl:
-
Sodium chloride.
- NaOH:
-
Sodium hydroxide.
- NMA:
-
Normal melting point agarose.
- PBS:
-
Phosphate buffered saline.
- PEEP:
-
Positive end-expiratory pressure.
- PO2 :
-
Partial pressure of oxygen.
- ROS:
-
Reactive oxygen species.
- RPM:
-
Revolutions per minute.
- SD:
-
Standard Deviation.
- TDNAP:
-
Tail DNA percentage parameter.
- V:
-
Volt.
- VAP:
-
Ventilator-associated pneumonia.
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The study was conducted according to the Declaration of Helsinki and followed ethical principles. Freely given informed consent to participate and consent to publish were obtained from the participants or their relatives. Ethical approval was obtained from Süleyman Demirel University, Faculty of Medicine, Clinical Research Ethics Committee with number 229 dated 13/12/2018, and study permission was obtained from Isparta City Hospital.
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Dilek Aşcı Çelik
Conceptualization, Methodology, Investigation, Project administration, Formal analysis, Data curation, Resources, Validation, Supervision, Visualization, Writing - original draft.
Vehbi Atahan Toğay
Investigation, Formal analysis, Data curation, Resources, Visualization, Writing - original draft.
Pınar Karabacak
Conceptualization, Investigation, Project administration, Validation, Writing – review & editing.
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Aşci Çelik, D., Toğay, V.A. & Karabacak, P. DNA damage assessment in pneumonia patients treated in the intensive care unit. Biologia 77, 1909–1913 (2022). https://doi.org/10.1007/s11756-022-01050-6
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DOI: https://doi.org/10.1007/s11756-022-01050-6