Abstract
The Cerrado biodiversity has been threatened by uncontrolled anthropization, which makes essential the propagation of native species for conservation purposes. This study aims to propagate Justicia lanstyakii, a Ni-accumulating subshrub, by cuttings, micropropagation, and plantlet formation. Hence, J. lanstyakii plants were collected in September (dry season) and December (rainy season). For cuttings, stem segments treated with IBA were inoculated in either sand or vermiculite. For in vitro propagation, the explants from mature plants were decontaminated with Carbendazim, ethanol, and either Na- or Ca-hypochlorite. Explant multiplication was accomplished in two consecutive subcultures in MS medium with BAP (0.0, 0.01 and 0.1 mg.L−1). Rooting was done with explants treated with IBA (0.0 and 0.01 mg.L−1) in MS. For J. lanstyakii plantlet formation, stem segments from seven distinct matrices were inoculated in ¼MS with BAP (1 and 2 mg.L−1). The results showed that the cuttings collected in December responded significantly better than those from September. Rooting and sprouting rates of December cuttings were 98.6% and produced 5.8 and 2.5 roots/shoots per explant, respectively. Furthermore, phytoregulators and substrates had no effects on cuttings. For in vitro propagation, Ca-hypochlorite showed higher explant decontamination and survival rates. Also, phytoregulators had no significant effects on multiplication and rooting. Nonetheless, the subcultures positively affected multiplication and rooting. However, plantlet development was highly influenced by the plant matrix and BAP (1 and 2 mg.L−1). Thus, all three techniques are suitable to propagate J. lanstyakii, which will facilitate its use in bioremediation programs of Ni-contaminated areas.
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This study was funded by Coordination of Improvement of Higher Education Personnel (CAPES), Federal District Research Support Foundation (FAPDF, grant No 0193.000941/2015), and Graduate Program in Botany of the University of Brasilia, and the Reference Centre of Nature Conservation and Recuperation of Degraded Areas of the University of Brasilia (CRAD).
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Mayara Rodrigues Lima and Conceição Eneida dos Santos Silveira performed and designed experiments, analyzed and interpreted data and wrote the manuscript. Hugo Teixeira Gomes helped with the designing of the experiments, analyzed, and interpreted data. Natalia Faustino Cury assisted in plantlet formation analysis. Luiz Alfredo Rodrigues Pereira helped with the anatomical analysis, corrected, and revised the manuscript.
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Lima, M.R., Gomes, H.T., Cury, N.F. et al. Developing propagation protocols for Justicia lanstyakii Rizz. (Acanthaceae), an ornamental Ni-accumulating subshrub of Brazilian Cerrado. Biologia 77, 967–980 (2022). https://doi.org/10.1007/s11756-021-00987-4
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DOI: https://doi.org/10.1007/s11756-021-00987-4