Abstract
Microalgae can accumulate a high amount of lipids under stress conditions, and, for this reason, it is of great interest in biodiesel production. To explore another promising candidate, growth, pigments, and biochemical composition of Lobochlamys sp. GUEco1006 on various growth media viz. Bold’s Basal Medium (BBM), Bristol, BG11 (Blue-green 11 medium), and Chu’s 10 were studied initially. The alga grown on BBM showed the best growth with maximum carotenoid content (4.8 µg⁄ml), lipid content (22.27%), and lipid productivity (61.5 mg L− 1d− 1). Further, the effect of salinity on pigments, lipid accumulation, and productivity of the test organism in the BBM medium was explored. The microalga attained significantly higher carotenoid content (11.13 µg⁄ml), lipid content (81.2% per dry cell weight), and lipid productivity (188.8 mg L− 1d− 1) in 0.3 M NaCl treated culture over control. This study demonstrated that salinity could act as a lipid trigger for Lobochlamys sp. GUEco1006, and it can be a promising candidate for biodiesel production in the near future.
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References
Alishah Aratboni H, Rafiei N, Garcia-Granados R, Alemzadeh A, Morones-Ramirez JR (2019) Biomass and lipid induction strategies in microalgae for biofuel production and other applications. Microb Cell Fact 18:178. https://doi.org/10.1186/s12934-019-1228-4
BenMoussa-Dahmen I, Chtourou H, Rezgui F, Sayadi S, Dhouib A (2016) Salinity stress increases lipid, secondary metabolites and enzyme activity in Amphora subtropica and Dunaliella sp. for biodiesel production. Bioresour Technol 218:816–825. https://doi.org/10.1016/j.biortech.2016.07.022
Bischoff HW, Bold HC (1963) Phycological studies IV. Some soil algae from enchanted rock and related algal species. University of Texas, Austin 6318:1–95
Chanklong K, Chotigeat W, Iewkittayakorn J (2018) Effects of culture medium on growth kinetics and fatty acid composition of Chlorella sp. T12. Songklanakarin J Sci Technol 40(5):1098–1104
Chtourou H, Dahmen I, Jebali A, Karray F, Hassairi I, Abdelkafi S, Ayadi H, Sayadi S, Dhouib A (2015) Characterization of Amphora sp., a newly isolated diatom wild strain, potentially usable for biodiesel production. Bioprocess Biosyst Eng 38:1381–1392. https://doi.org/10.1007/s00449-015-1379-6
Chu S (1942) The influence of the mineral composition of the medium on the growth of planktonic algae: Part I. Methods and culture media. J Ecol 30(2):284–325
Colusse GA, Mendes CRB, Duarte MER, de Carvalho JC, Noseda MD (2020) Effects of different culture media on physiological features and laboratory scale production cost of Dunaliella salina. Biotechnol Rep 27:e00508. https://doi.org/10.1016/j.btre.2020.e00508
Fakhry EM, El Maghraby DM (2015) Lipid accumulation in response to nitrogen limitation and variation of temperature in Nannochloropsis salina. Bot Stud 56:6. https://doi.org/10.1186/s40529-015-0085-7
Fan J, Huang J, Li Y, Han F, Wang J, Li X, Wang W, Li S (2012) Sequential heterotrophy-dilution-photoinduction cultivation for efficient microalgal biomass and lipid production. Bioresour Technol 112:206–211. doi:https://doi.org/10.1016/j.biortech.2012.02.046
Fan J, Cui Y, Wan M, Wang W, Li Y (2014) Lipid accumulation and biosynthesis genes response of the oleaginous Chlorella pyrenoidosa under three nutrition stressors. Biotechnol Biofuels 7:17. https://doi.org/10.1186/1754-6834-7-17
Felsenstein J (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39:783–791
Fjerbaek L, Christensen KV, Norddahl B (2009) A review of the current state of biodiesel production using enzymatic transesterification. Biotechnol Bioeng 102:1298–1315. https://doi.org/10.1002/bit.22256
Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497–509
Ge S, Champagne P (2016) Nutrient removal, microalgal biomass growth, harvesting and lipid yield in response to centrate wastewater loadings. Water Res 88:604–612. https://doi.org/10.1016/j.watres.2015.10.054
Griffiths MJ, Harrison STL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493–507. https://doi.org/10.1007/s10811-008-9392-7
Ho SH, Nakanishi A, Ye X, Chang JS, Hara K, Hasunuma T, Kondo A (2014) Optimizing biodiesel production in marine Chlamydomonas sp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy. Biotechnol Biofuels 7:97. https://doi.org/10.1186/1754-6834-7-97
Hounslow E, Kapoore RV, Vaidyanathan S, Gilmour DJ, Wright PC (2016) The Search for a Lipid Trigger: The Effect of Salt Stress on the Lipid Profile of the Model Microalgal Species Chlamydomonas reinhardtii for Biofuels Production. Curr Biotechnol 5:305–313. https://doi.org/10.2174/2211550105666160322234434
Ilavarasi A, Mubarakali D, Praveenkumar R, Baldev E, Thajuddin N (2011) Optimization of various growth media to freshwater microalgae for biomass production. Biotechnol 10(6):540–545. https://doi.org/10.3923/biotech.2011.540.545
Lichtenthaler HK (1987) Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. In: Methods in Enzymology, Plant Cell Membranes, Academic, Cambridge, pp 350–382. https://doi.org/10.1016/0076-6879(87)48036-1
Lohman EJ, Gardner RD, Pedersen T, Peyton BM, Cooksey KE, Gerlach R (2015) Optimized inorganic carbon regime for enhanced growth and lipid accumulation in Chlorella vulgaris. Biotechnol Biofuels 8:82. https://doi.org/10.1186/s13068-015-0265-4
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Lu C, Vonshak A (2002) Effects of salinity stress on photosystem II function in cyanobacterial Spirulina platensis cells. Physiol Plant 114:405–413
Pal D, Khozin-Goldberg I, Cohen Z, Boussiba S (2011) The effect of light, salinity, and nitrogen availability on lipid production by Nannochloropsis sp. Appl Microbiol Biotechnol 90:1429–1441. https://doi.org/10.1007/s00253-011-3170-1
Paliwal C, Pancha I, Ghosh T, Maurya R, Chokshi K, Vamsi Bharadwaj SV, Ram S, Mishra S (2015) Selective carotenoid accumulation by varying nutrient media and salinity in Synechocystis sp. CCNM 2501. Bioresour Technol 197:363–368. https://doi.org/10.1016/j.biortech.2015.08.122
Pancha I, Chokshi K, Maurya R, Trivedi K, Patidar SK, Ghosh A, Mishra S (2015) Salinity induced oxidative stress enhanced biofuel production potential of microalgae Scenedesmus sp. CCNM 1077. Bioresour Technol 189:341–348. https://doi.org/10.1016/j.biortech.2015.04.017
Pröschold T, Marin B, Schlösser UG, Melkonian M (2001) Molecular phylogeny and taxonomic revision of Chlamydomonas (Chlorophyta). I. Emendation of Chlamydomonas ehrenberg and Chloromonas gobi, and Description of Oogamochlamys gen. nov. and Lobochlamys gen. nov. Protist 152:265–300
Rai M, Gautom T, Sharma N (2015) Effect of salinity, pH, light intensity on growth and lipid production of microalgae for bioenergy application. Online J Biol Sci 15(4):260–267. https://doi.org/10.3844/OJBSCI.2015.260.267
Ren HY, Liu BF, Ma C, Zhao L, Ren NQ (2013) A new lipid-rich microalga Scenedesmus sp. strain R-16 isolated using Nile red staining: effects of carbon and nitrogen sources and initial pH on the biomass and lipid production. Biotechnol Biofuels 6:143. https://doi.org/10.1186/1754-6834-6-143
Rodolfi L, Chini Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100–112. https://doi.org/10.1002/bit.22033
Saitou N, Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic tress. Mol Biol Evol 4:406–425
Salama ES, Kim HC, Abou-Shanab RAI, Ji MK, Oh YK, Kim SH, Jeon BH (2013) Biomass, lipid content, and fatty acid composition of freshwater Chlamydomonas mexicana and Scenedesmus obliquus grown under salt stress. Bioprocess Biosyst Eng 36:827–833. https://doi.org/10.1007/s00449-013-0919-1
Sangapillai K, Marimuthu T (2019) Isolation and selection of growth medium for freshwater microalgae Asterarcys quadricellulare for maximum biomass production. Water Sci Technol 80:2027–2036
Spiro RG (1966) Analysis of sugars found in glycoproteins. In: Methods in Enzymology, Complex Carbohydrates. Academic, Cambridge, pp 3–26. https://doi.org/10.1016/0076-6879(66)08005-4
Srivastava G, Nishchal, Goud VV (2017) Salinity induced lipid production in microalgae and cluster analysis (ICCB 16-BR_047). Bioresour Technol 242:244–252. https://doi.org/10.1016/j.biortech.2017.03.175
Sudhir P, Murthy SDS (2004) Effects of salt stress on basic processes of photosynthesis. Photosynthetica 42:481–486
von Alvensleben N, Magnusson M, Heimann K (2016) Salinity tolerance of four freshwater microalgal species and the effects of salinity and nutrient limitation on biochemical profiles. J Appl Phycol 28:861–876
Wong YK, Ho YH, Ho KC, Leung HM, Yung KKL (2017) Growth medium screening for Chlorella vulgaris growth and lipid production. J Aquac Mar Biol 6(1):00143
Xia L, Rong J, Yang H, He Q, Zhang D, Hu C (2014) NaCl as an effective inducer of lipid accumulation in freshwater microalgae Desmodesmus abundans. Bioresour Technol 161:402–409
Zabochnicka-Świątek M, Kamizela T, Kowalczyk M, Kalaji HM, Bąba W (2019) Inexpensive and universal growth media for biomass production of microalgae. Glob Nest J 21(1):82–89
Acknowledgements
We are thankful to the Head, Department of Botany, Gauhati University, Assam, India, for providing laboratory facilities developed under DST-FIST, UGC-SAP program, and MoEF & CC to carry out the research work.
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Author 1. Hunmily Teronpi: Conceptualization; Data curation; Formal analysis; Investigation; Methodology; Validation; Visualization; Roles/Writing - original draft. Author 2. Prof. Partha Pratim Baruah: Supervision; Writing - review & editing. Author 3. Hemchandra Deka: Software.
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Teronpi, H., Baruah, P.P. & Deka, H. Salinity stress as a critical factor to trigger lipid accumulation in a freshwater microalga Lobochlamys sp. GUEco1006. Biologia 76, 3647–3658 (2021). https://doi.org/10.1007/s11756-021-00918-3
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DOI: https://doi.org/10.1007/s11756-021-00918-3