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Nondestructive estimation of fatty acid composition in seeds of Brassica napus L. by near-infrared spectroscopy

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Journal of the American Oil Chemists' Society

Abstract

The feasibility of near-infrared (NIR) spectroscopy for the nondestructive determination of fatty acid composition in rapeseed was examined. NIR spectra were measured on extracted oil, intact rapeseed kernels, and an intact signle rapeseed with an InfraAlyzer 500 in a syrup cup or a single-grain cup. NIR spectra were scanned from 1100 to 2500 nm at 2-nm intervals. As the percentage of linoleic acid increased, the spectral values in the region 1696–1724 nm, where linoleic acid has its absorption band, became always stronger downward in second-derivative NIR spectra. As the percentage of erucic acid increased the spectral value at 1728 nm, where erucic acid has its absorption band, became always a little bit stronger downward in the second-derivative NIR spectra. On the basis of their NIR spectral patterns, linoleic acid and erucic acid could be successfully determined in both intact seed kernels and in a single seed of rape without damaging them.

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References

  1. Sonntag, N.O.V., Erucic, Behenic: Feedstocks of the 21st Century, INFORM 2:449–463 (1991).

    Google Scholar 

  2. Daun, J.K., The Introduction of Low Erucic Acid Rapeseed Varieties into Canadian, in High and Low Erucic Acid Rapeseed Oils, edited by J.K. Kramer and F. Saer, Academic Press, Toronto, 1983, pp. 161–180.

    Google Scholar 

  3. Daun, J.K., Modified Fatty Acid Profiles in Canadian Oilseeds, J. Jpn. Oil Chem. Soc. 17(3):233–238 (1998).

    Google Scholar 

  4. Shibata, M., I. Kaneko, S. Sugawara, and T. Endo, On the Improvement of Fatty Acid Composition in Rapeseed—I. Fatty Acid Composition in Seed Oil of Japanese and Zero-Erucic Acid Varieties, and the Distribution of Erucic Acid Content in Their F2 Seeds, Tohoku Agric. Res. 29:91–92 (1981).

    Google Scholar 

  5. Sato, T., S. Kawano, and M. Iwamoto, Detection of Foreign Fat Adulteration of Milk Fat by Near-Infrared Spectroscopic Method, J. Dairy Sci. 73:3408–3413 (1990).

    Article  Google Scholar 

  6. Sato, T., S. Kawano, and M. Iwamoto, Near-Infrared Spectral Patterns of Fatty Acid Analysis from Fats and Oils, J. Am. Oil Chem. Soc. 68:827–833 (1991).

    CAS  Google Scholar 

  7. Sato, T., Application of Principal-Component Analysis on Near-Infrared Spectroscopic Data of Vegetable Oils for Their Classification, Ibid.:293–298 (1994).

    Article  CAS  Google Scholar 

  8. Sato, T., Y. Takahata, T. Noda, T. Yanagisawa, T. Morishita, and S. Sakai, Nondestructive Determination of Fatty Acid Composition of Husked Sunflower (Helianthus annua L.) Seeds by Near-Infrared Spectroscopy, Ibid.:1177–1183 (1995).

    Article  CAS  Google Scholar 

  9. Karen, M.B., A.D. Clarke, C. Roberts, and N. Unklesbay, Discriminant Analysis of Vegetable Oils by Near-Infrared Reflectance Spectroscopy, Ibid.:195–200 (1994).

    Article  Google Scholar 

  10. Chikuni, K., S. Ozawa, T. Mitsuhasi, M. Mitsumoto, T. Koishikawa, S. Kato, and K. Ozutsumi, Effects of Nonadrenaline Injections on the Composition of Plasma Free Fatty Acids in Beef Cattle, Jpn. J. Zootech. Sci. 60:29–34 (1989).

    CAS  Google Scholar 

  11. Starr, C., A.G. Morgan, and D.B. Smith, An Evaluation of Near-Infrared Reflectance Analysis in Some Plant Breeding Programmes, J. Agric. Sci. 97:107–118 (1981).

    Article  Google Scholar 

  12. Tkachuk, R., Oil and Protein Analysis of Whole Rapeseed Kernels by Near-Infrared Reflectance, J. Am. Oil Chem. Soc. 57:819–822 (1981).

    Google Scholar 

  13. Velasco, L., J. Fernández-Martínez, and A. De Haro, Screening Ethiopian Mustard for Erucic Acid by Near Infrared Reflectance Spectroscopy, Crop Sci. 36:1068–1071 (1996).

    Article  Google Scholar 

  14. Velasco, L., J.M. Fernández-Martínez, and A. De Haro, Determination of the Fatty Acid Composition of the Oil in Intact-Seed Mustard by Near-Infrared Reflectance Spectroscopy, J. Am. Oil Chem. Soc. 74:1595–1602 (1997).

    Article  CAS  Google Scholar 

  15. Reinhardt, T.-C., and G. Röbbelen, Quantitative Analysis of Fatty Acids in Intact Rapeseed by Near Infrared Reflectance Spectroscopy, in Proceedings of the 8th International Rapeseed Conference, Saskatoon, Canada, 9–11 July 1991, edited by Groupe Consultatif International de Recherche sur le Colza (GCIRC), Saskatoon, pp. 1380–1384.

  16. Daun, J.K., K.M. Clear, and P.C. Williams, Comparison of Three Whole Seed Near-Infrared Analyzers for Measuring Quality Components of Canola Seeds, J. Am. Oil Chem. Soc. 71:266–282 (1994).

    Article  Google Scholar 

  17. Daun, J.K., and P.C. Williams, Near Infrared Analysis of Oilseeds—Current Status and Future Directions, in New Techniques and Applications in Lipid Analysis, edited by R. McDonald and M. Mossoba, AOCS Press, Champaign, 1997, pp. 266–282.

    Google Scholar 

  18. Yamazaki, M., A. Nagao, H. Kasamo, and M. Kimura, The Characteristics and Qualities of Seed Oils from Various Rapes Grown in Japan, Nippon Shokuhin Kogyo Gakkaishi, 31:619–623 (1984).

    CAS  Google Scholar 

  19. Downey, R.K., and B.L. Harvey, Methods of Breeding for Oil Quality in Rape, Can. J. Plant Sci. 43:271–275 (1963).

    Article  CAS  Google Scholar 

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Correspondence to Tetsuo Sato.

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Sato, T., Uezono, I., Morishita, T. et al. Nondestructive estimation of fatty acid composition in seeds of Brassica napus L. by near-infrared spectroscopy. J Amer Oil Chem Soc 75, 1877–1881 (1998). https://doi.org/10.1007/s11746-998-0344-9

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  • DOI: https://doi.org/10.1007/s11746-998-0344-9

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