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Gas chromatography-fourier transform infrared spectrometry of fatty acids: New applications with a direct deposition interface

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Journal of the American Oil Chemists' Society

Abstract

Infrared spectroscopy is a suitable spectroscopic method to differentiate geometric Z and E isomers of unsaturated compounds. A direct-deposition Fourier transform infrared spectrometer (FTIR), coupled to a gas chromatograph, was used successfully to analyze with a high sensitivity traces of C18:1 fatty acid methyl ester (FAME) isomers. It could also conclusively distinguish between isomers of conjugated diunsaturated FAME. The achievable sensitivity of this direct-deposition device makes possible accurate FAME mixture analyses that are not currently attainable with the more conventional light-pipe interface.

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References

  1. Jensen, N.J., and M.L. Gross, Mass Spectrometry Methods for Structural Determination and Analysis of Fatty Acids, Mass Spectrom. Rev. 6:497–536 (1987).

    Article  CAS  Google Scholar 

  2. Griffiths, P.R., and J.A. de Haseth, Fourier Transform Infrared Spectrometry, Wiley-Interscience, New York, 1986, p. 656.

    Google Scholar 

  3. Herres, W., HRGC-FTIR: Chromatography-Fourier Transform Infrared Spectroscopy. Theory and Applications, Hüthig, Heidelberg, 1987, p. 212.

    Google Scholar 

  4. Le Quéré, J.L., Le Couplage CPG/IRTF dans l’Analyse des Arômes, Analusis 20:M46-M49 (1992).

    Google Scholar 

  5. Le Quéré, J.L., Gas Chromatography-Fourier Transform Infrared Spectrometry in the Analysis of Fatty Acids, in New Trends in Lipid and Lipoprotein Analyses, edited by J.L. Sébédio and E.G. Perkins, AOCS Press, Champaign, 1995, Ch. 17.

    Google Scholar 

  6. Sébédio, J.L., J.L. Le Quéré, E. Sémon, O. Morin, J. Prévost, and A. Grandgirard, Heat Treatment of Vegetable Oils. II. GC-MS and GC-FTIR Spectra of Some Isolated Cyclic Fatty Acid Monomers, J. Am. Oil Chem. Soc. 64:1324–1333 (1987).

    Article  Google Scholar 

  7. Grandgirard, A., A. Piconneaux, J.L. Sébédio, S.F. O’Keefe, E. Sémon, and J.L. Le Quéré, Occurrence of Geometrical Isomers of Eicosapentaenoic and Docosahexaenoic Acids in Liver Lipids of Rats Fed Heated Linseed Oil, Lipids 24:799–804 (1989).

    Article  PubMed  CAS  Google Scholar 

  8. Christie, W.W., E.Y. Brechany, J.L. Sébédio, and J.L. Le Quéré, Silver Ion Chromatography and Gas Chromatography-Mass Spectrometry in the Structural Analysis of Cyclic Monoenoic Acids Formed in Frying Oils, Chem. Phys. Lipids 66:143–153 (1993).

    Article  CAS  Google Scholar 

  9. Dobson, G., W.W. Christie, E.Y. Brechany, J.L. Sébédio, and J.L. Le Quéré, Silver Ion Chromatography and Gas Chromatography-Mass Spectrometry in the Structural Analysis of Cyclic Dienoic Acids Formed in Frying Oils, Ibid.:171–182 (1995).

    Article  CAS  Google Scholar 

  10. Reedy, G.T., D.G. Ettinger, J.F. Schneider, and S. Bourne, High-Resolution Gas Chromatography/Matrix Isolation Infrared Spectrometry, Anal. Chem. 57:1602–1609 (1985).

    Article  CAS  Google Scholar 

  11. Mossoba, M.M., R.E. McDonald, J.Y.T. Chen, D.J. Armstrong, and S.W. Page, Identification and Quantitation of trans-9,trans-12-Octadecadienoic Acid Methyl Ester and Related Compounds in Hydrogenated Soybean Oil and Margarines by Capillary Gas Chromatography/Matrix Isolation/Fourier Transform Infrared Spectroscopy, J. Agric. Food Chem. 38:86–92 (1990).

    Article  CAS  Google Scholar 

  12. Bourne, S., A.M. Haefner, K.L. Norton, and P.R. Griffiths, Performance Characteristics of a Real-Time Direct Deposition Gas Chromatography/Fourier Transform Infrared Spectrometry, Anal. Chem. 62:2448–2452 (1990).

    Article  CAS  Google Scholar 

  13. Visser, T., and M.J. Vredenbregt, Cryotrapping: Gas Chromatography-Fourier Transform Infrared Spectrometry in Environmental Analysis: A Pilot Study, Vib. Spectrosc. 1:205–210 (1990).

    Article  CAS  Google Scholar 

  14. Ferary, S., J. Auger, and A. Touché, Trace Identification of Plant Substances by Combining Gas Chromatography-Mass Spectrometry and Direct-Deposition Gas Chromatography-Fourier Transform Infrared Spectrometry, Talanta 43:349–357 (1996).

    Article  CAS  Google Scholar 

  15. Gilbert, A.S., C.J. Moss, P.L. Francis, M.J. Ashton, and D.S. Ashton, Combined Gas Chromatography-Infra-Red Spectroscopy for the Determination of Propanediol in Acyclovir Cream, Chromatographia 42:305–308 (1996).

    Article  CAS  Google Scholar 

  16. Chardigny, J.M., R.L. Wolff, E. Mager, J.L. Sébédio, L. Martine, and P. Juanéda, Trans Mono- and Polyunsaturated Fatty Acids in Human Milk, Eur. J. Clin. Nutr. 49:523–531 (1995).

    PubMed  CAS  ADS  Google Scholar 

  17. Carreau, J.P., and J.P. Dubacq, Adaptation of a Macro-Scale Method to the Micro-Scale for Fatty Acid Methyl Transesterification of Biological Lipid Extracts, J. Chromatogr. 151:384–390 (1978).

    Article  CAS  Google Scholar 

  18. Mossoba, M.M., R.E. McDonald, D.J. Armstrong, and S.W. Page, Identification of Minor C18 Triene and Conjugated Diene Isomers in Hydrogenated Soybean Oil and Margarine by GC-MI-FT-IR Spectroscopy, J. Chromatogr. Sci. 29:324–330 (1991).

    PubMed  CAS  Google Scholar 

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Correspondence to E. Sémon.

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Sémon, E., Ferary, S., Auger, J. et al. Gas chromatography-fourier transform infrared spectrometry of fatty acids: New applications with a direct deposition interface. J Amer Oil Chem Soc 75, 101–105 (1998). https://doi.org/10.1007/s11746-998-0018-7

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  • DOI: https://doi.org/10.1007/s11746-998-0018-7

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