Abstract
Infrared spectroscopy is a suitable spectroscopic method to differentiate geometric Z and E isomers of unsaturated compounds. A direct-deposition Fourier transform infrared spectrometer (FTIR), coupled to a gas chromatograph, was used successfully to analyze with a high sensitivity traces of C18:1 fatty acid methyl ester (FAME) isomers. It could also conclusively distinguish between isomers of conjugated diunsaturated FAME. The achievable sensitivity of this direct-deposition device makes possible accurate FAME mixture analyses that are not currently attainable with the more conventional light-pipe interface.
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Sémon, E., Ferary, S., Auger, J. et al. Gas chromatography-fourier transform infrared spectrometry of fatty acids: New applications with a direct deposition interface. J Amer Oil Chem Soc 75, 101–105 (1998). https://doi.org/10.1007/s11746-998-0018-7
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DOI: https://doi.org/10.1007/s11746-998-0018-7