Skip to main content
SpringerLink
Log in

Separation and quantitation of cholesterol oxides by HPLC with an evaporative light scattering detector in a model system

  • Published:
Journal of the American Oil Chemists' Society

Abstract

A method to analyze cholesterol and 10 of its oxidation products, ranging from the weakly polar cholest-4-ene-3,6-dione to moderately polar cholest-5-ene-3β,7α-diol, in a single run is described. The separation was achieved by normal-phase gradient high-performance liquid chromatography with an evaporative light-scattering detector. This universal mass detector does not detect changes in solvent composition; this makes it possible to employ gradients, an essential technique whenever a wide range of compounds with diverse characteristics is to be separated. Standards at concentrations from 0.1–1.0 µg were separated within 37 min on an alumina/silica column with a gradient elution system that contained dichloromethane, acetonitrile, and water.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Peng, S.-K., and C.B. Taylor, Cholesterol Autoxidation, Health and Arteriosclerosis: A Review on Situations in Developed Countries, World Rev. Nutr. Diet 44:117–154 (1984).

    PubMed  CAS  Google Scholar 

  2. Bischoff, F., and G. Byron. The Pharmacodynamics of and Toxicology of Steroids and Related Compounds, Adv. Lipid Res. 15:96–102 (1977).

    Google Scholar 

  3. Sevanian, A., and A.R. Peterson, Cholesterol Epoxide Is a Direct-Acting Mutagen, Proc. Nat. Acad. Sci. USA 81:4198–4202 (1984).

    Article  PubMed  CAS  ADS  Google Scholar 

  4. Teng, J.I., M.J. Kulig, and L.L. Smith, Gas Chromatographic Differentiation Among Cholesterol Hydroperoxides, J. Chromatogr. 75:108–113 (1973).

    Article  PubMed  CAS  Google Scholar 

  5. Smith, L.L., in Cholesterol Autoxidation, Plenum Press, New York, 1981, pp. 5–6.

    Google Scholar 

  6. Tsai, L.S., K. Ijichi, C.A. Hudson, and C.C. Meehan, A Method for the Quantitative Estimation of Cholesterol A-Oxide in Eggs, Lipids 15:124–128 (1980).

    Article  CAS  Google Scholar 

  7. Chicoye, E., W.D. Powrie, and O. Fennema, Photoxidation of Cholesterol in Spray Dried Egg Yolk upon Irradiation, J. Food Sci. 33:581–587 (1968).

    Article  CAS  Google Scholar 

  8. Moreau, R.A., Quantitative Analysis of Lipids by HPLC with a Flame Ionization Detector or an Evaporative Light-Scattering Detector, in Lipid Chromatographic Analysis, edited by T. Shibamoto, Marcel Dekker, Inc., New York, 1994, Chapter 7, pp. 251–272.

    Google Scholar 

  9. Stolyhwo, A., D. Colin, and G. Guiochon, Use of Light Scattering as a Detector Principle in Liquid Chromatography, J. Chromatogr. 265:1–18 (1983).

    Article  CAS  Google Scholar 

  10. Mourey, T.H., and L.E. Oppenheimer, Principles of Operation of an Evaporative Light Scattering Detector for Liquid Chromatography, Anal. Chem. 56:2427–2434 (1984).

    Article  CAS  Google Scholar 

  11. Mourey, T.H., and L.E. Oppenheimer, Examination of the Concentration Response of Evaporative Light Scattering Mass Detectors, J. Chromatogr. 323:297–304 (1985).

    Article  Google Scholar 

  12. Stolyhwo, A., M. Martin, and G. Guiochon. Analysis of Lipid Classes by HPLC with Evaporative Light Scattering Detector, J. Liquid Chromatogr. 10:1237–1253 (1987).

    CAS  Google Scholar 

  13. Spanos, G.A., and S.J. Schwartz, Determination of Cholesterol in Milk Fat by Reversed Phase HPLC and Evaporative Light

Download references

Author information

Authors and Affiliations

  1. USDA, ARS, ERRC, 600 East Mermaid Lane, 19038, Wyndmoor, PA

    L. Lakritz & K. C. Jones

Authors
  1. L. Lakritz
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. C. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to L. Lakritz.

About this article

Cite this article

Lakritz, L., Jones, K.C. Separation and quantitation of cholesterol oxides by HPLC with an evaporative light scattering detector in a model system. J Amer Oil Chem Soc 74, 943–946 (1997). https://doi.org/10.1007/s11746-997-0008-1

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-997-0008-1

Key words

Search

Navigation