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Modern Methods of Analysis of Carotenoids (Review)

  • STRUCTURE OF CHEMICAL COMPOUNDS, METHODS OF ANALYSIS AND PROCESS CONTROL
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Pharmaceutical Chemistry Journal Aims and scope

Qualitative and quantitative methods of analysis of carotenoids that are used to analyze drugs, dietary supplements, and food products are reviewed. UV and visible spectrophotometry and chromatographic methods, e.g., thin-layer (TLC), high-performance liquid (HPLC), and supercritical fluid (SFC) chromatography, are discussed. The importance of using hybrid chromatography methods with IR, NMR, and mass-spectroscopic detectors is demonstrated. The dependence of carotenoid chromatographic separation parameters on sorbate polarity (dipole moment) in TLC, HPLC, and SFC is demonstrated for the first time.

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References

  1. O. Isler (ed.), Carotenoids, Springer, Basel (1971).

    Google Scholar 

  2. O. Straub, Key to Carotenoids, Birkhauser, Basel (1987).

    Book  Google Scholar 

  3. C. H. Eugster, “History: 175 Years of Carotenoid Chemistry,” in: Carotenoids, Vol. 1A: Isolation and Analysis, G. Britton, S. Liaaen-Jensen, and H. Pfander (eds.), Birkhauser, Basel, Switzerland (1995), pp. 1 – 12.

  4. G. Britton, “UV / Visible Spectroscopy,” in: Carotenoids, Vol. 1B: Spectroscopy, G. Britton, S. Liaaen-Jensen, and H. Pfander (eds.), Birkhauser, Basel (1995), pp. 13 – 62.

  5. K. T. Amorim-Carrilho, A. Cepeda, C. Fente, and P. Regal, Trends Anal. Chem., 56, 49 – 73 (2014).

    Article  CAS  Google Scholar 

  6. D. B. Rodriguez-Amaya, A Guide to Carotenoid Analysis in Foods, ILSI Press, Washington, D. C. (2001).

    Google Scholar 

  7. D. B. Rodriguez-Amaya and M. Kimura, Harvest Plus Handbook for Carotenoid Analysis, ILSI Press, Washington, D. C. (2004).

    Google Scholar 

  8. R. E. Kopec, J. L. Cooperstone, M. J. Cichen, and S. G. Schwartz, “Analysis Methods of Carotenoids,” in: Analysis of Antioxidant-rich Phytochemicals, Z. Xu and L. R. Howard (eds.), John Wiley & Sons Ltd., The Atrium, Southern Gate (2012), pp. 105 – 148.

    Chapter  Google Scholar 

  9. R. A. Kellner, J.-M. Mermet, M. Otto, and H. M. Widmer (eds.), Analytical Chemistry, Wiley VCH, Weinheim (1998) [Russian translation, Yu. A. Zolotov (ed.), Mir, Moscow (2004), pp. 596 – 638].

  10. OFS 1.2.3.00017.15. “Methods for quantitative determination of vitamins (draft)” [in Russian], RF State Pharmacopoeia, XIIIth Ed., Vol. 1, Moscow (2015), pp. 798 – 811.

  11. USP 32-NF27.

  12. Beta Carotene, Revision Bulletin, Official December, 1 (2012).

  13. Betacarotene, in: EP 6.0, v. 2, 1290 (2008).

  14. Betacarotene, in: BP (2009).

  15. D. Horn and E. Luddecke, “Preparation and characterization of nano-sized carotenoid hydrosols,” in: Fine Particles Science and Technology: From Micro to Nanoparticles, Proceedings of the NATO Advanced Research Workshop, Acquafredda di Maratea, Italy, Jul. 15 – 21 (1995); E. Pelizzetti (ed.), Kluwer Academic Publishers, Dordrecht, Boston, London (1966), pp. 761 – 775.

  16. A. N. Macpherson and T. Gillbro, J. Phys. Chem. A, 102(26), 5049 – 5058 (1998).

    Article  CAS  Google Scholar 

  17. K. J. Scott, “Detection and measurement of carotenoids by UV / Vis spectrophotometry,” F2.2.1-F2.2.10, in: Current Protocols in Food Analytical Chemistry, John Wiley & Sons (2001), p. 740.

  18. B. D. Ribeiro and R. G. de Oliveira, “Carotenoids as colorants,” in: Natural Products Phytochemistry. Botany and Metabolism of Alkaloids, Phenolics and Terpenes, K. G. Ramawat and J.-M. Merillon (eds.), Springer, New York (2013), pp. 4017 – 4036.

    Google Scholar 

  19. E. Biehler, F. E. Mayer, L. Hoffmann, et al., J. Food Sci., 75(1), 55 – 61 (2010).

    Article  Google Scholar 

  20. A. V. Rao and L. G. Rao, Pharmacol. Res., 55, 207 – 216 (2007).

    Article  CAS  PubMed  Google Scholar 

  21. M. J. O’Neil, A. Smith, and P. E. Heckelman (eds.), The MERCK Index: An Encyclopedia of Chemicals, Drugs and Biologicals, 11th Ed., Merck Co., Inc., Rahway, N. J. (1996).

    Google Scholar 

  22. F. Demirkaya, Y. Kadioglu, and O. Senol, Rev. Anal. Chem., 29(3 – 4), 149 – 168 (2010).

    CAS  Google Scholar 

  23. A. B. Barua, H. C. Furr, J. A. Olson, and R. B. van Breemen, “Vitamin A and carotenoids,” in: Modern Chromatographic Analysis of Vitamins, A. P. De Leenheer, W. E. Lambert, and J. F. Van Bocxlaer (eds.), Marcel Dekker, New York (2000), pp. 13 – 86

    Google Scholar 

  24. G. W. Francis and O. M. Andersen, “Natural pigments. Carotenoids,” in: Handbook of Thin-Layer Chromatography, 3rd Ed., Marcel Dekker, New York (2003), pp. 708 – 714.

  25. B. Fried and J. Sherma, Thin-Layer Chromatography, 4th Ed., Revised and Expanded, Marcel Dekker, New York (1999).

  26. K. Schiedt, “Chromatography, Part III, Thin-layer chromatography,” in: Carotenoids, Vol. 1A, Isolation and Analysis, G. Britton, S. Liaaen-Jensen, and H. Pfander (eds.), Brikhauser, Basel (1995), pp. 131 – 144.

    Google Scholar 

  27. G. Britton, “TLC of carotenoids,” in: Thin Layer Chromatography in Phytochemistry, M. Waksmundzka-Hajnos, J. Sherma, and T. Kowalska (eds.), CRC Press, New York (2008), pp. 543 – 573.

    Google Scholar 

  28. V. D. Krasikov, Principles of Planar Chromatography [in Russian], Khimizdat, St. Petersburg (2005).

    Google Scholar 

  29. S. W. Jeffrey, Biochim. Biophys. Acta, 162, 271 – 285 (1968), “Quantitative thin-layer chromatography of chlorophylls and carotenoids from marine algae.”

  30. M. Isaksen and G. W. Francis, J. Chromatogr., 355, 358 – 362 (1986).

    Article  CAS  Google Scholar 

  31. G. W. Francis and M. Isaksen, J. Food Sci., 53(3), 979 – 980 (1988).

    Article  CAS  Google Scholar 

  32. A. Zeb and M. Murkovic, J. Planar Chromatogr., 23(2), 94 – 103 (2010).

    Article  CAS  Google Scholar 

  33. A. Zeb and M. Murkovic, J. Planar Chromatogr., 23(1), 35 – 39 (2010).

    Article  CAS  Google Scholar 

  34. M. Isaksen and G. W. Francis, Chromatographia, 29(7/8), 363 – 365 (1990).

    Article  CAS  Google Scholar 

  35. A. J. R. Romero, J. C. Herrera, E. M. De Aparicio, and E. A. M. Cuevad, J. Chromatogr. A, 667, 361 – 366 (1994).

    Article  Google Scholar 

  36. B. Yuangsoi, O. Jintasataporn, N. Areechon, et al., Songklanakarin J. Sci. Technol., 30(6), 693 – 700 (2008).

    Google Scholar 

  37. M. M. Goto, “Methods for extraction and analysis of carotenoids,” in: Natural Products. Phytochemistry, Botany and Metabolism of Alkaloids, Phenolics and Terpenes, K. G. Ramawat and J.-M. Merillon (eds.), Springer, New York (2013), pp. 3367 – 3411.

    Google Scholar 

  38. A. V. I. Hess, J. Chem. Educ., 84(5), 842 – 847 (2007).

    Article  CAS  Google Scholar 

  39. T. Tuzimski, “Basic principles of planar chromatography and its potential for hyphenated techniques,” in: High-Performance Thin-Layer Chromatography (HPTLC),M. M. Srivastava (ed.), Springer, Berlin (2011), pp. 247 – 310.

    Chapter  Google Scholar 

  40. T. Tuzimski, “Advanced spectroscopic detectors for identification and quantification: UV-visible, fluorescence, and infrared spectroscopy,” in: Instrumental Thin-Layer Chromatography, C. F. Poole (ed.), Elsevier, Amsterdam, London (2015), pp. 239 – 248.

    Chapter  Google Scholar 

  41. S. Cheng and J. Shiea, “Advanced spectroscopic detectors for identification and quantification: Mass spectrometry,” in: Instrumental Thin-Layer Chromatography, C. F. Poole (ed.), Elsevier, Amsterdam, London (2015), pp. 249 – 277.

    Chapter  Google Scholar 

  42. I. Vovk and V. Glavnik, “Analysis of dietary supplements,” in: Instrumental Thin-Layer Chromatography, C. F. Poole (ed.), Elsevier, Amsterdam, London (2015), pp. 606 – 610.

    Google Scholar 

  43. Z. Rodic, B. Simonovska, A. Albreht, and I. Vovk, J. Chromatogr. A, 1231, 59 – 65 (2012).

    Article  CAS  PubMed  Google Scholar 

  44. E. I. Finkel’shtein, N. A. Mednikova, E. V. Alekseev, and E. I. Kozlov, Khim.-farm. Zh., 11(1), 106 – 111 (1977); Pharm. Chem. J., 11(1), 100 – 104 (1977).

  45. I. S. Krasnokutskaya and E. I. Finkelshtein, J. Mol. Struct., 349, 313 – 316 (1995).

    Article  CAS  Google Scholar 

  46. B. Spangenberg and K.-F. Klein, J. Chromatogr. A, 898, 265 – 269 (2000).

    Article  CAS  PubMed  Google Scholar 

  47. S. A. Stahlmann, J. Planar Chromatogr. – Mod. TLC, 12(1), 5 – 12 (1999).

    CAS  Google Scholar 

  48. C. Cimpoiu, J. Liq. Chromatogr. Relat. Technol., 28(7 – 8), 1203 – 1213 (2005).

    Article  CAS  Google Scholar 

  49. A. J. R. Romero, J. C. Herrera, E. M. De Aparicio, and E. A. M. Cuevas, J. Chromatogr. A, 667, 361 – 366 (1999).

    Article  Google Scholar 

  50. H. Pfander and R. Riesen, “Chromatography, Part IV, High-Performance Liquid Chromatography,” in: Carotenoids. Vol. 1A, Isolation and Analysis, G. Britton, S. Liaaen-Jensen, and H. Pfander (eds.), Birkhauser, Basel, Switzerland (1995), pp. 145 – 190.

    Google Scholar 

  51. R. F. Taylor, P. E. Farrow, L. M. Yelle, et al., “Advances in HPLC and HPLC-MS of carotenoids and retinoids,” in: Carotenoids. Chemistry and Biology, N. I. Krinsky, M. M. Mathews-Roth, and R. F. Taylor (eds.), Plenum Press, New York (1990), pp. 105 – 123.

    Google Scholar 

  52. S. M. Rivera and R. Canela-Garayoa, J. Chromatogr. A, 1224, 1 – 10 (2012).

    Article  CAS  PubMed  Google Scholar 

  53. E. I. Finkel’shtein, E. A. Belozertseva, E. V. Trifonova, et al., Khim.-farm. Zh., 47(6), 39 – 43 (2013); Pharm. Chem. J., 47(6), 325 – 329 (2013).

  54. H. J. C. F. Nelis and A. P. De Leenheer, Anal. Chem., 55(2), 270 – 275 (1983).

    Article  CAS  Google Scholar 

  55. A. M. Pupin, M. J. Dennis, and M. C. F. Toledo, Food Chem., 64, 269 – 275 (1999).

    Article  CAS  Google Scholar 

  56. R. J. Bushway, J. Liq. Chromatogr., 8(8), 1527 – 1547 (1985).

    Article  CAS  Google Scholar 

  57. R. Aman, J. Biehl, R. Carle, et al., Food Chem., 92, 753 – 763 (2005).

    Article  CAS  Google Scholar 

  58. H. J. C. F. Nelis, M. M. Z. van Steenberge, M. F. Lefevere, and A. P. de Leenheer, J. Chromatogr., 353, 295 – 302 (1986).

    Article  CAS  Google Scholar 

  59. G. Englert, K. Noack, E. A. Broger, et al., Helv. Chim. Acta, 74, 969 – 982 (1991).

    Article  CAS  Google Scholar 

  60. K. S. Epler, L. C. Sander, R. G. Ziegler, et al., J. Chromatogr., 595, 89 – 101 (1992).

    Article  CAS  PubMed  Google Scholar 

  61. T. H. Kao, C. H. Loh, B. S. Inbaraj, and B. H. Chen, J. Pharm. Biomed. Anal., 66, 144 – 153 (2012).

    Article  CAS  PubMed  Google Scholar 

  62. A. J. Melendez-Martinez, M. L. Escudero-Gilete, I. M. Vicario, and F. J. Heredia, Food Res. Int., 43, 1289 – 1296 (2010).

    Article  CAS  Google Scholar 

  63. A. Gentili, F. Caretti, S. Ventura, et al., J. Agric. Food Chem., 63(33), 7428 – 7439 (2015).

    Article  CAS  PubMed  Google Scholar 

  64. H. G. Daood, G. Bencze, G. Palotes, et al., J. Chromatogr. Sci., 52(9), 985 – 991 (2014).

    Article  PubMed  Google Scholar 

  65. L. C. Sander, K. E. Sharpless, and M. Pursch, J. Chromatogr. A, 880, 189 – 202 (2000).

    Article  CAS  PubMed  Google Scholar 

  66. I. F. Strati, V. J. Sinanoglou, L. Kora, et al., Foods, 1, 52 – 65 (2012).

    Article  Google Scholar 

  67. T. Glaser and K. Albert, “Hyphenation of modern extraction techniques to LC-NMR for the analysis of geometrical carotenoids isomers in functional food and biological tissues,” in: On-Line LC-NMR and Related Techniques, Wiley, Atrium (2002), pp. 129 – 140.

  68. M. Dachtler, T. Glaser, K. Kohler, and K. Albert, Anal. Chem., 73(3), 667 – 674 (2001).

    Article  CAS  PubMed  Google Scholar 

  69. C. Tode, T. Maoka, and M. Sugiura, J. Sep. Sci., 32(21), 3659 – 3663 (2009).

    Article  CAS  PubMed  Google Scholar 

  70. C. Tode and M. Sugiura, “LC-NMR for the analysis of carotenoids in foods,” in: Vitamin A and Carotenoids Chemistry, Analysis, Function and Effects, V. R. Preedy (ed.), RSC, Cambridge (2012), pp. 250 – 260.

  71. C. Tode and M. Sugiura, “LC-NMR for the analysis of carotenoids in foods,” in: Vitamin A and Carotenoids Chemistry, Analysis, Function and Effects, V. R. Preedy (ed.), RSC, Cambridge (2012), pp. 269 – 281, 282 – 302.

  72. A. Guillou, G. Chouberff, and J. de la Nolle, Food Chem., 476, 93 – 99 (1993).

    Article  Google Scholar 

  73. H. Pfander and U. Niggli, “Chromatography, Part V, Supercritical-fluid chromatography,” in: Carotenoids, Vol. 1A, Isolation and Analysis, G. Britton, S. Liaaen-Jensen, and H. Pfander (eds.), Brikhauser, Basel, Switzerland (1995), pp. 191 – 198.

    Google Scholar 

  74. E. Lesellier, J. Chromatogr. A, 936, 201 – 214 (2001).

    Article  CAS  PubMed  Google Scholar 

  75. V. Abrahamsson, I. Rodriguez-Meizoso, and C. Turner, J. Chromatogr. A, 1250, 63 – 68 (2012).

    Article  CAS  PubMed  Google Scholar 

  76. J. L. Bernal, M. T. Martin, and L. Toribio, J. Chromatogr. A, 1313, 24 – 36 (2013).

    Article  CAS  PubMed  Google Scholar 

  77. A. Matsubara, T. Uchikata, M. Shinohara, et al., J. Biosci. Bioeng., 113, No. 6, 782 – 787 (2012).

    Article  CAS  PubMed  Google Scholar 

  78. Y. Wada, A. Matsubara, T. Uchikata, et al., J. Sep. Sci., 34, 3546 – 3552 (2011).

    Article  CAS  PubMed  Google Scholar 

  79. C. Horvath, W. Melander, and I. Molnar, J. Chromatogr., 1, 129 – 156 (1976).

    Article  Google Scholar 

  80. C. Horvath and W. Melander, J. Chromatogr. Sci., 15(9), 393 – 404 (1977).

    Article  CAS  Google Scholar 

  81. C. Horvath, W. Melander, and I. Molnar, Anal. Chem., 49(1), 142 – 154 (1977).

    Article  CAS  Google Scholar 

  82. J. Cazes and R. P. W. Scott, Chromatography Theory, Marcel Dekker, New York (2002), pp. x+475.

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Correspondence to E. I. Finkel’shtein.

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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 50, No. 2, pp. 29 – 40, February, 2016.

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Finkel’shtein, E.I. Modern Methods of Analysis of Carotenoids (Review). Pharm Chem J 50, 96–107 (2016). https://doi.org/10.1007/s11094-016-1405-2

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