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Destabilization of Egg Yolk Emulsion After IgY Removal Through Enzymatic Treatments

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  • Published:
Journal of the American Oil Chemists' Society

Abstract

The objective of this study was to destabilize the protein–lipid complex in egg yolk precipitate obtained after the removal of soluble proteins, referred to as the pellet, through enzymatic treatment for further phospholipids extraction. A combination of proteolytic and lipolytic enzymes was applied to release the lipids from the pellet or weaken the pellet emulsion. Emulsions prepared using Protease P/Lipase AY30, Protease II/Lipase AY30 and Protease M/Lipase AY30 treated pellets had larger oil droplets (78, 65, 56 µm) and higher coalescence rates (51, 41, 35 %) than those of Protex 51FP, pellet, Protex 7L and Protease A with oil droplet size of 20, 18, 15 and 13 µm and coalescence rates of 31, 8, 7.5 and 8 %, respectively. Cream and liquid subnatant fractions obtained after further centrifugation of hydrolysates were subjected to lipid analyses. Over 90 % of phosphatidylcholine (PC) present in the pellet and 80 % of that in the original egg yolk were recovered in the cream from Protease P/Lipase AY30 treatment, while the recovery of PC from the egg yolk was significantly lower in creams from Protex 7L or Protease 51FP treatments (12 and 10 %, respectively). Pellets treated with Protease M, Protex 7L or Protex 51FP in combination with Lipase AY30 led to a significant loss of PC due to the conversion of PC to lysophosphatidylcholine or its degradation. Cream fractions obtained from the study represented a better material for the recovery of PL than intact egg yolk using environmentally-friendly techniques such as supercritical carbon dioxide (SC-CO2) extraction.

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Acknowledgments

This work was supported by grants from the Alberta Livestock Meat Agency, Ltd. (ALMA), Alberta Innovates - Bio Solutions (Al Bio), and Natural Sciences and Engineering Research Council of Canada (NSERC).

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Authors and Affiliations

  1. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, T6G2P5, Canada

    Sahar Navidghasemizad, Alexandra Acero-Lopez, Jonathan Curtis, Feral Temelli & Jianping Wu

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  1. Sahar Navidghasemizad
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  2. Alexandra Acero-Lopez
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  3. Jonathan Curtis
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  4. Feral Temelli
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  5. Jianping Wu
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Correspondence to Feral Temelli or Jianping Wu.

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Navidghasemizad, S., Acero-Lopez, A., Curtis, J. et al. Destabilization of Egg Yolk Emulsion After IgY Removal Through Enzymatic Treatments. J Am Oil Chem Soc 91, 1857–1866 (2014). https://doi.org/10.1007/s11746-014-2542-y

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  • DOI: https://doi.org/10.1007/s11746-014-2542-y

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