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Compositional and Antioxidant Activity Analysis of Zanthoxylum bungeanum Seed Oil Obtained by Supercritical CO2 Fluid Extraction

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Journal of the American Oil Chemists' Society

Abstract

Supercritical CO2 fluid extraction (SFE-CO2) of Zanthoxylum bungeanum (Z. bungeanum) seed oil was investigated. To optimize the SFE process, three-level Box-Behnken factorial design and response surface methodology (RSM) were applied to optimize the extraction conditions, including pressure, temperature and amount of modifier. The optimum conditions were as follows: extraction pressure, 29.28 MPa; extraction temperature, 41.19 °C; and the added amount of modifier, 10.94%. The experimental results showed that the maximum extraction yield was 21.85 ± 0.23% (n = 3) under the proposed conditions. The compositional analysis of Z. bungeanum seed oil was performed by HPLC-FLD-MS using a new labeling reagent of 2-(11H-benzo[a]carbazol-11-yl)-ethyl-4-methyl benzenesulfonate (BCETS). The results indicated that the Z. bungeanum seed oil contained mainly unsaturated fatty acids, including C18:3, C22:6, C20:4, C18:2, C18:1 and C20:1, which accounted for 84.0% (mass percentage) of the total amount. The antioxidant activity of seed oil obtained by Box-Behnken design concerning the DPPH radical was investigated, and this indicated that the pressure and the amount of added modifier had positive effects on the antioxidant activity, but the effect of the temperature elevation is complicated, depending on the nature of the extracted contents.

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Acknowledgments

This work was supported by the National Science Foundation of China (no. 20075016) and supported by the 100 Talents Programme of The Chinese Academy of Sciences (No. 328).

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Authors and Affiliations

  1. Northwest Plateau Institute of Biology, The Chinese Academy of Sciences, Xining, 810001, China

    Lian Xia, Jinmao You, Guoliang Li, Zhiwei Sun & Yourui Suo

  2. Key Laboratory of Life-Organic Analysis of Shan Dong Province, College of Chemistry Science, Qufu Normal University, Qufu, 273165, China

    Lian Xia & Jinmao You

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Lian Xia, Guoliang Li & Zhiwei Sun

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  1. Lian Xia
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  2. Jinmao You
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  3. Guoliang Li
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  4. Zhiwei Sun
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Correspondence to Jinmao You.

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Xia, L., You, J., Li, G. et al. Compositional and Antioxidant Activity Analysis of Zanthoxylum bungeanum Seed Oil Obtained by Supercritical CO2 Fluid Extraction. J Am Oil Chem Soc 88, 23–32 (2011). https://doi.org/10.1007/s11746-010-1644-4

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  • DOI: https://doi.org/10.1007/s11746-010-1644-4

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