Abstract
The total phenolic content and the antioxidant potential of methanolic extract (ME), ethyl acetate extract (EAE), and hexane extract (HE) from Monechma ciliatum leaves (MCL) were evaluated. The Folin-Ciocalteu, β-carotene bleaching, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and the accelerated oxidation methods were used for evaluation. Both the extraction yield and the antioxidant activity (AOA) were strongly dependent on the solvent. Among the extracts, ME exhibited highest total phenolic compounds (TPC) and IC50 values for DPPH, followed by EAE and HE, respectively. Peroxide value (PV), anisidine value (AV) conjugated dienes (CD), and thiobarbituric acid reactive substances (TBARS) were taken as the parameters for evaluation of stabilization efficacy of MCL extracts and results revealed MCL to be a potent antioxidant for the stabilization of corn oil. As a general trend, increased AOA was observed for increased extract concentration. The predominant phenolic compounds identified by HPLC-DAD in MCL extracts were p-coumaric acid, vanillin and ferulic acid.
Similar content being viewed by others
References
Surh YJ (2002) Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem Toxicol 40:1091–1097
Jadhav SJ, Nimbalkar SS, Kulkarni AD, Madhavi DL (1996) Lipid oxidation in biological and food systems. In: Madhavi DL, Deshpande SS, Salunkhe DK (eds) Food antioxidants. Marcel Dekker, New York, pp 5–63
Ramarathnam N, Ochi H, Takeuchi M (1997) Antioxidant defense system in vegetable extracts. In: Shahidi F (ed) Natural antioxidants: chemistry health effects and applications. AOCS Press, Champaign, pp 76–87
Mariod AA, Matthaus B, Hussein IH (2008) Antioxidant properties of methanolic extracts from different parts of Sclerocarya birrea. Int J Food Sci Technol 43:921–926
Kaur R, Arora S, Singh B (2008) Antioxidant activity of the phenol rich fractions of leaves of Chukrasia tabularis A. Juss Bioresour Technol 99:7692–7698
Braca A, Sanogo M, Sanou R, Morelli H, Pizza I, Tommasi N (2003) Chemical composition and antioxidant activity of phenolic compounds from wild and cultivated Sclerocarya birrea (Anacardiaceae) leaves. J Agric Food Chem 51:6689–6695
Halliwell B, Gutteridge JMC (1985) Free radicals, ageing, and disease, free radicals in biology and medicine, 2nd edn. Clarendron, Oxford, pp 279–315
Gray JI (1978) Measurement of lipid oxidation, a review. J Am Oil Chem Soc 55:539–546
St Angelo AJ, Ory RL, Brown LE (1975) Comparison of methods for determining peroxidation in processed whole peanut products. J Am Oil Chem Soc 52:34–35
Sharief MT (2002) The effect of sowing date and plant spacing on growth and yield of black mahlab (Monechma ciliatum). M.Sc. Thesis, University of Khartoum, Khartoum, Sudan
Ayoub SM, Babiker AJ (1981) Component fatty acids from the oils of Monechma ciliatum. Fitoterapia 52:251–253
Uguru MO, Okwuasaba FK, Ekwenchi MM, Uguru VE (1995) Oxytocic and oestrogenic effects of Monechma ciliatum methanol extract in vivo and in vitro in rodents. Phytother Res 9:26–29
Mariod AA, Ibrahim RM, Ismail M, Ismail N (2010) Antioxidant activities of phenolic rich fractions (PRFs) obtained from black mahlab (Monechma ciliatum) and white mahlab (Prunus mahaleb) seedcakes. Food Chem 118:120–127
Iqbal S, Haleem S, Akhtar M, Zia-ul-Haq M, Akbar J (2008) Efficiency of pomegranate peel extracts in stabilization of sunflower oil under accelerated conditions. Food Res Int 41:194–200
Gordon MH, Paiva-Martins F, Almeida M (2001) Antioxidant activity of hydroxytyrosol acetate compared with that of other olive oil polyphenols. J Agric Food Chem 49:2480–2485
Amarowicz R, Karamac M, Shahidi F (2003) Antioxidant activity of phenolic fractions of lentil (Lens culinaris). J Food Lipids 10:1–10
AOCS (2000) Official methods and recommended practices of the American Oil Chemists’ Society, 5th edn. Official method Cc 13b-45, AOCS, Champaign (reapproved)
Roussis IG, Tzimas PC, Soulti K (2008) Antioxidant activity of white wine extracts and some phenolic acids toward corn oil oxidation. J Food Process Preserv 32:535–545
Shahidi F, Desilva C, Amarowicz R (2003) Antioxidant activity of extracts of defatted seeds of Niger (Guizotia abyssinica). J Am Oil Chem Soc 80:443–450
Chirinos R, Betalleluz I, Humána A, Arbizub C, Pedreschi R, Campos C (2009) HPLC-DAD characterization of phenolic compounds from Andean oca (Oxalis tuberose Mol.) tubers and their contribution to the antioxidant capacity. Food Chem 113:1243–1251
Mariod AA, Matthaüs B, Eichner K, Hussein IH (2006) Antioxidant activity of extracts from Sclerocarya birrea kernel oil cake. Grasas Y Aceites 57:361–366
Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53:1841–1856
Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to antioxidant activity. LWT 26:25–30
Nagai T, Reiji I, Hachiro I, Nobutaka S (2003) Preparation and antioxidant properties of water extract of propolis. Food Chem 80:29–33
Yang JH, Lin HC, Mau JL (2002) Antioxidant properties of several commercial mushrooms. Food Chem 77:229–235
Chew YL, Lima YY, Omara M, Khoob KS (2008) Antioxidant activity of three edible sea weeds from two areas in South East Asia. LWT 41:1067–1072
Othman A, Ismail A, Abdul Ghani N, Adenan I (2007) Antioxidant capacity and phenolic content of cocoa beans. Food Chem 100:1523–1530
Leontowicz M, Gorinstein S, Leontowicz H, Krzminski R, Lojek A, Katrich E (2003) Apple and pear peel and pulp and their influence on plasma lipids and antioxidant potentials in rats fed cholesterol-containing diets. J Agric Food Chem 51:5780–5785
Duh PD, Yen YC (1997) Antioxidant efficacy of methanolic extracts of peanut hulls in soybean and peanut oils. J Am Oil Chem Soc 74:745–748
Acknowledgments
The first author is grateful to IBS/UPM for the senior research fellowship granted during the period of this work. We thank Siti Muskinah, Laboratory of Molecular Biomedicine IBS/UPM for analysis in HPLC-DAD equipment and excellent technical expertise needed for this work. This study was funded by Intensifying Research Priority Areas (IRPA) grants provided by Government of Malaysia and Universiti Putra Malaysia.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Mariod, A.A., Ibrahim, R.M., Ismail, M. et al. Antioxidant Activity of the Phenolic Leaf Extracts from Monechma ciliatum in Stabilization of Corn Oil. J Am Oil Chem Soc 87, 35–43 (2010). https://doi.org/10.1007/s11746-009-1474-4
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11746-009-1474-4