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Monooxygenase system of Bacillus megaterium ALA2: Studies on palmitic acid oxidation products

  • Published:
Journal of the American Oil Chemists' Society

Abstract

We identified many novel oxygenated FA produced from linoleic acid by microbial strain ALA2: 12,13,17-trihydroxy-9(Z)-octadecenoic acid (12,13,17-THOA); 12,13,16-trihydroxy-9(Z)-octadecenoic acid (12,13,16-THOA); 12-hydroxy-13,16-epoxy-9(Z)-octadecenoic acid; and 12,17;13,17-diepoxy-16-hydroxy-9(Z)-octadecenoic acid. 12,13,17-THOA, the main product, inhibits the growth of some plant pathogenic fungi. Recently, we reclassified strain ALA2 as Bacillus megaterium ALA2 NRRL B-21660 and opened a possible link with the well-studied catalytically self-sufficient P450 monooxygenase of Bacillus megaterium ATCC 14581 (NRRL B-3712) and B. subtilis strain 168 (NRRL B-4219). Now we have found that strain ALA2 also oxidizes palmitic acid into three oxygenated products: 13-, 14-, and 15-hydroxy palmitic acids. This indicates that strain ALA2 also possesses a monooxygenase system similar to the abovementioned well-known strains. These data facilitate studies on the oxygenase system of strain ALA2.

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  1. Microbial Genomics and Bioprocessing Research Unit, NCAUR, ARS, USDA, 1815 North University St., 61604, Peoria, IL

    Ching T. Hou

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Correspondence to Ching T. Hou.

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Hou, C.T. Monooxygenase system of Bacillus megaterium ALA2: Studies on palmitic acid oxidation products. J Am Oil Chem Soc 82, 839–843 (2005). https://doi.org/10.1007/s11746-005-1152-8

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  • DOI: https://doi.org/10.1007/s11746-005-1152-8

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