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Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling

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Lipids

Abstract

The objective of this study was to establish the impact of caloric restriction on high fat diet-induced alterations on regulators of skeletal muscle growth. We hypothesized that caloric restriction would reverse the negative effects of high fat diet-induced obesity on REDD1 and mTOR-related signaling. Following an initial 8 week period of HF diet-induced obesity, caloric restriction (CR ~30 %) was employed while mice continued to consume either a low (LF) or high fat (HF) diet for 8 weeks. Western analysis of skeletal muscle showed that CR reduced (p < 0.05) the obesity-related effects on the lipogenic protein, SREBP1. Likewise, CR reduced (p < 0.05) the obesity-related effects on the hyperactivation of mTORC1 and ERK1/2 signaling to levels comparable to the LF mice. CR also reduced (p < 0.05) obesity-induced expression of negative regulators of growth, REDD1 and cleaved caspase 3. These findings have implications for on the reversibility of dysregulated growth signaling in obese skeletal muscle, using short-term caloric restriction.

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Abbreviations

CR:

Caloric restriction

ERK:

Extracellular signal-regulated kinase

HF:

High fat

LF:

Low fat

MEK:

MAPK/ERK kinase

mTOR:

Mammalian target of rapamycin

mTORC1:

mTOR complex 1

REDD1:

Regulated in development and DNA damage responses 1

rpS6:

Ribosomal protein S6, S6K1 p70 ribosomal protein S6 kinase-1

SREBP1c:

Sterol regulatory element-binding protein 1c

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Acknowledgments

The authors would like to thank Mingxia Cui for excellent support throughout the project.

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Authors and Affiliations

  1. Department of Exercise and Nutrition Sciences, School of Public Health and Health Professions, University at Buffalo, SUNY, 2 Sherman Hall (Office)/5 Sherman Hall, Buffalo, NY, 14214, USA

    Cory M. Dungan & David L. Williamson

  2. Department of Pharmacology and Toxicology, School of Medicine, University at Buffalo, Buffalo, NY, USA

    Ji Li

  3. Kinesiology Program, School of Behavioral Sciences and Education, Penn State Harrisburg, Middletown, PA, USA

    David L. Williamson

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  1. Cory M. Dungan
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  2. Ji Li
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Correspondence to David L. Williamson.

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Dungan, C.M., Li, J. & Williamson, D.L. Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling. Lipids 51, 905–912 (2016). https://doi.org/10.1007/s11745-016-4168-3

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