Abstract
Skeletal muscle plays a major role in glucose homeostasis. Ectopic lipid accumulation in non-adipose tissue, including skeletal muscle, is an important feature of insulin resistance and type 2 diabetes. Leptin, an adipocyte-derived hormone, regulates glucose and lipid metabolism in skeletal muscle, independent of its central effects on food intake and energy expenditure. While in vitro evidence shows that leptin interacts with insulin signaling to enhance glucose uptake in skeletal muscle, in vivo studies indicate that enhanced insulin sensitivity and glucose uptake induced by leptin in skeletal muscle are mediated by a central mechanism. On the other hand, a direct effect of leptin on skeletal muscle lipid metabolism has been demonstrated in mice. Leptin stimulates mitochondrial fatty acid oxidation by directly activating 5′-AMP-activated protein kinase, through which it depletes the lipid content and reduces lipotoxicity in skeletal muscle. In a mouse model of obesity and diabetes, the regenerative capacity of skeletal muscle is impaired, causing sarcopenia. Evidence from several mouse studies indicates that leptin signaling regulates not only the lipid content of muscle but also muscle mass by inhibiting protein degradation and enhancing myoblast proliferation and differentiation. Thus, impaired leptin action might be a key factor in the negative regulation of skeletal muscle regeneration and the development of sarcopenia in obesity and diabetes. In this chapter, we discuss the current understanding of leptin’s effects on glucose and lipid metabolism in skeletal muscle and on muscle mass maintenance and regeneration in the context of obesity and type 2 diabetes.
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References
Ahima RS, Flier JS (2000) Leptin. Annu Rev Physiol 62:413–437
Myers MG Jr (2004) Leptin receptor signaling and the regulation of mammalian physiology. Recent Prog Horm Res 59:287–304
Kielar D, Clark JS, Ciechanowicz A, Kurzawski G, Sulikowski T, Naruszewicz M (1998) Leptin receptor isoforms expressed in human adipose tissue. Metab Clin Exp 47(7):844–847
Guerra B, Santana A, Fuentes T, Delgado-Guerra S, Cabrera-Socorro A, Dorado C et al (2007) Leptin receptors in human skeletal muscle. J Appl Physiol 102(5):1786–1792
Kieffer TJ, Heller RS, Habener JF (1996) Leptin receptors expressed on pancreatic beta-cells. Biochem Biophys Res Commun 224(2):522–527
Bjorbaek C, Kahn BB (2004) Leptin signaling in the central nervous system and the periphery. Recent Prog Horm Res 59:305–331
Denroche HC, Huynh FK, Kieffer TJ (2012) The role of leptin in glucose homeostasis. J Diabetes Investig 3(2):115–129
Margetic S, Gazzola C, Pegg GG, Hill RA (2002) Leptin: a review of its peripheral actions and interactions. Int J Obes Relat Metab Disord 26(11):1407–1433
Morioka T, Asilmaz E, Hu J, Dishinger JF, Kurpad AJ, Elias CF et al (2007) Disruption of leptin receptor expression in the pancreas directly affects beta cell growth and function in mice. J Clin Invest 117(10):2860–2868
Pelleymounter MA, Cullen MJ, Baker MB, Hecht R, Winters D, Boone T et al (1995) Effects of the obese gene product on body weight regulation in ob/ob mice. Science 269(5223):540–543
Goodpaster BH, Wolf D (2004) Skeletal muscle lipid accumulation in obesity, insulin resistance, and type 2 diabetes. Pediatr Diabetes 5(4):219–226
Kahn BB, Flier JS (2000) Obesity and insulin resistance. J Clin Invest 106(4):473–481
Bates SH, Gardiner JV, Jones RB, Bloom SR, Bailey CJ (2002) Acute stimulation of glucose uptake by leptin in l6 muscle cells. Horm Metab Res 34(3):111–115
Berti L, Gammeltoft S (1999) Leptin stimulates glucose uptake in C2C12 muscle cells by activation of ERK2. Mol Cell Endocrinol 157(1–2):121–130
Berti L, Kellerer M, Capp E, Haring HU (1997) Leptin stimulates glucose transport and glycogen synthesis in C2C12 myotubes: evidence for a P13-kinase mediated effect. Diabetologia 40(5):606–609
Kellerer M, Koch M, Metzinger E, Mushack J, Capp E, Haring HU (1997) Leptin activates PI-3 kinase in C2C12 myotubes via janus kinase-2 (JAK-2) and insulin receptor substrate-2 (IRS-2) dependent pathways. Diabetologia 40(11):1358–1362
Ceddia RB, William WN Jr, Curi R (1999) Comparing effects of leptin and insulin on glucose metabolism in skeletal muscle: evidence for an effect of leptin on glucose uptake and decarboxylation. Int J Obes Relat Metab Disord 23(1):75–82
Harris RB (1998) Acute and chronic effects of leptin on glucose utilization in lean mice. Biochem Biophys Res Commun 245(2):502–509
Muoio DM, Dohm GL, Fiedorek FT Jr, Tapscott EB, Coleman RA (1997) Leptin directly alters lipid partitioning in skeletal muscle. Diabetes 46(8):1360–1363
Burcelin R, Kamohara S, Li J, Tannenbaum GS, Charron MJ, Friedman JM (1999) Acute intravenous leptin infusion increases glucose turnover but not skeletal muscle glucose uptake in ob/ob mice. Diabetes 48(6):1264–1269
Kamohara S, Burcelin R, Halaas JL, Friedman JM, Charron MJ (1997) Acute stimulation of glucose metabolism in mice by leptin treatment. Nature 389(6649):374–377
Unger RH, Clark GO, Scherer PE, Orci L (2010) Lipid homeostasis, lipotoxicity and the metabolic syndrome. Biochim Biophys Acta 1801(3):209–214
Kelley DE, Goodpaster BH (2001) Skeletal muscle triglyceride. An aspect of regional adiposity and insulin resistance. Diabetes Care 24(5):933–941
Steinberg GR, Parolin ML, Heigenhauser GJ, Dyck DJ (2002) Leptin increases FA oxidation in lean but not obese human skeletal muscle: evidence of peripheral leptin resistance. Am J Physiol Endocrinol Metab 283(1):E187–E192
Levin N, Nelson C, Gurney A, Vandlen R, de Sauvage F (1996) Decreased food intake does not completely account for adiposity reduction after ob protein infusion. Proc Natl Acad Sci U S A 93(4):1726–1730
Shimabukuro M, Koyama K, Chen G, Wang MY, Trieu F, Lee Y et al (1997) Direct antidiabetic effect of leptin through triglyceride depletion of tissues. Proc Natl Acad Sci U S A 94(9):4637–4641
Muoio DM, Dohm GL, Tapscott EB, Coleman RA (1999) Leptin opposes insulin’s effects on fatty acid partitioning in muscles isolated from obese ob/ob mice. Am J Physiol 276(5 Pt 1):E913–E921
Suzuki A, Okamoto S, Lee S, Saito K, Shiuchi T, Minokoshi Y (2007) Leptin stimulates fatty acid oxidation and peroxisome proliferator-activated receptor alpha gene expression in mouse C2C12 myoblasts by changing the subcellular localization of the alpha2 form of AMP-activated protein kinase. Mol Cell Biol 27(12):4317–4327
Minokoshi Y, Kahn BB (2003) Role of AMP-activated protein kinase in leptin-induced fatty acid oxidation in muscle. Biochem Soc Trans 31(Pt 1):196–201
Minokoshi Y, Kim YB, Peroni OD, Fryer LG, Muller C, Carling D et al (2002) Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase. Nature 415(6869):339–343
Hardie DG (2007) AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy. Nat Rev Mol Cell Biol 8(10):774–785
Hayashi T, Hirshman MF, Fujii N, Habinowski SA, Witters LA, Goodyear LJ (2000) Metabolic stress and altered glucose transport: activation of AMP-activated protein kinase as a unifying coupling mechanism. Diabetes 49(4):527–531
Hayashi T, Hirshman MF, Kurth EJ, Winder WW, Goodyear LJ (1998) Evidence for 5′ AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport. Diabetes 47(8):1369–1373
Mu J, Brozinick JT Jr, Valladares O, Bucan M, Birnbaum MJ (2001) A role for AMP-activated protein kinase in contraction- and hypoxia-regulated glucose transport in skeletal muscle. Mol Cell 7(5):1085–1094
Winder WW, Hardie DG (1999) AMP-activated protein kinase, a metabolic master switch: possible roles in type 2 diabetes. Am J Physiol 277(1 Pt 1):E1–E10
Long YC, Zierath JR (2006) AMP-activated protein kinase signaling in metabolic regulation. J Clin Invest 116(7):1776–1783
Olsen GS, Hansen BF (2002) AMP kinase activation ameliorates insulin resistance induced by free fatty acids in rat skeletal muscle. Am J Physiol Endocrinol Metab 283(5):E965–E970
Munzberg H, Myers MG Jr (2005) Molecular and anatomical determinants of central leptin resistance. Nat Neurosci 8(5):566–570
Myers MG, Cowley MA, Munzberg H (2008) Mechanisms of leptin action and leptin resistance. Annu Rev Physiol 70:537–556
Steinberg GR, Dyck DJ (2000) Development of leptin resistance in rat soleus muscle in response to high-fat diets. Am J Physiol Endocrinol Metab 279(6):E1374–E1382
Dulloo AG, Stock MJ, Solinas G, Boss O, Montani JP, Seydoux J (2002) Leptin directly stimulates thermogenesis in skeletal muscle. FEBS Lett 515(1–3):109–113
Cruz-Jentoft AJ, Baeyens JP, Bauer JM, Boirie Y, Cederholm T, Landi F et al (2010) Sarcopenia: European consensus on definition and diagnosis: report of the European working group on sarcopenia in older people. Age Ageing 39(4):412–423
Kohara K (2014) Sarcopenic obesity in aging population: current status and future directions for research. Endocrine 45(1):15–25
Srikanthan P, Hevener AL, Karlamangla AS (2010) Sarcopenia exacerbates obesity-associated insulin resistance and dysglycemia: findings from the National Health and Nutrition Examination Survey III. PLoS One 5(5):e10805
Stenholm S, Harris TB, Rantanen T, Visser M, Kritchevsky SB, Ferrucci L (2008) Sarcopenic obesity: definition, cause and consequences. Curr Opin Clin Nutr Metab Care 11(6):693–700
Lim S, Kim JH, Yoon JW, Kang SM, Choi SH, Park YJ et al (2010) Sarcopenic obesity: prevalence and association with metabolic syndrome in the Korean Longitudinal Study on Health and Aging (KLoSHA). Diabetes Care 33(7):1652–1654
Abbatecola AM, Chiodini P, Gallo C, Lakatta E, Sutton-Tyrrell K, Tylavsky FA et al (2012) Pulse wave velocity is associated with muscle mass decline: health ABC study. Age 34(2):469–478
Zamboni M, Mazzali G, Fantin F, Rossi A, Di Francesco V (2008) Sarcopenic obesity: a new category of obesity in the elderly. Nutr Metab Cardiovasc Dis 18(5):388–395
Sakuma K, Yamaguchi A (2013) Sarcopenic obesity and endocrinal adaptation with age. Int J Endocrinol 2013:204164
Hubbard RE, O’Mahony MS, Calver BL, Woodhouse KW (2008) Nutrition, inflammation, and leptin levels in aging and frailty. J Am Geriatr Soc 56(2):279–284
Kohara K, Ochi M, Tabara Y, Nagai T, Igase M, Miki T (2011) Leptin in sarcopenic visceral obesity: possible link between adipocytes and myocytes. PLoS One 6(9):e24633
Waters DL, Qualls CR, Dorin RI, Veldhuis JD, Baumgartner RN (2008) Altered growth hormone, cortisol, and leptin secretion in healthy elderly persons with sarcopenia and mixed body composition phenotypes. J Gerontol A: Biol Med Sci 63(5):536–541
Dubey L, Hesong Z (2006) Role of leptin in atherogenesis. Exp Clin Cardiol 11(4):269–275
Casanueva FF, Dieguez C (1998) Interaction between body composition, leptin and growth hormone status. Bailliere Clin Endocrinol Metab 12(2):297–314
Dieguez C, Carro E, Seoane LM, Garcia M, Camina JP, Senaris R et al (2000) Regulation of somatotroph cell function by the adipose tissue. Int J Obes Relat Metab Disord 24(Suppl 2):S100–S103
Carbo N, Ribas V, Busquets S, Alvarez B, Lopez-Soriano FJ, Argiles JM (2000) Short-term effects of leptin on skeletal muscle protein metabolism in the rat. J Nutr Biochem 11(9):431–435
Akhmedov D, Berdeaux R (2013) The effects of obesity on skeletal muscle regeneration. Front Physiol 4:371
Wagers AJ, Conboy IM (2005) Cellular and molecular signatures of muscle regeneration: current concepts and controversies in adult myogenesis. Cell 122(5):659–667
Jang YC, Sinha M, Cerletti M, Dall’Osso C, Wagers AJ (2011) Skeletal muscle stem cells: effects of aging and metabolism on muscle regenerative function. Cold Spring Harb Symp Quant Biol 76:101–111
Snijders T, Verdijk LB, van Loon LJ (2009) The impact of sarcopenia and exercise training on skeletal muscle satellite cells. Ageing Res Rev 8(4):328–338
Cerletti M, Jang YC, Finley LW, Haigis MC, Wagers AJ (2012) Short-term calorie restriction enhances skeletal muscle stem cell function. Cell Stem Cell 10(5):515–519
Vignaud A, Ramond F, Hourde C, Keller A, Butler-Browne G, Ferry A (2007) Diabetes provides an unfavorable environment for muscle mass and function after muscle injury in mice. Pathobiol: J Immunopathol Mol Cell Biol 74(5):291–300
Tamilarasan KP, Temmel H, Das SK, Al Zoughbi W, Schauer S, Vesely PW et al (2012) Skeletal muscle damage and impaired regeneration due to LPL-mediated lipotoxicity. Cell Death Dis 3:e354
Purchas RW, Romsos DR, Allen RE, Merkel RA (1985) Muscle growth and satellite cell proliferative activity in obese (OB/OB) mice. J Anim Sci 60(3):644–651
Peterson JM, Bryner RW, Alway SE (2008) Satellite cell proliferation is reduced in muscles of obese Zucker rats but restored with loading. Am J Physiol Cell Physiol 295(2):C521–C528
Nguyen MH, Cheng M, Koh TJ (2011) Impaired muscle regeneration in ob/ob and db/db mice. Sci World J 11:1525–1535
Arounleut P, Bowser M, Upadhyay S, Shi XM, Fulzele S, Johnson MH et al (2013) Absence of functional leptin receptor isoforms in the POUND (Lepr(db/lb)) mouse is associated with muscle atrophy and altered myoblast proliferation and differentiation. PLoS One 8(8):e72330
Sainz N, Rodriguez A, Catalan V, Becerril S, Ramirez B, Gomez-Ambrosi J et al (2009) Leptin administration favors muscle mass accretion by decreasing FoxO3a and increasing PGC-1alpha in ob/ob mice. PLoS One 4(9):e6808
Hamrick MW, Herberg S, Arounleut P, He HZ, Shiver A, Qi RQ et al (2010) The adipokine leptin increases skeletal muscle mass and significantly alters skeletal muscle miRNA expression profile in aged mice. Biochem Biophys Res Commun 400(3):379–383
Drummond MJ, McCarthy JJ, Fry CS, Esser KA, Rasmussen BB (2008) Aging differentially affects human skeletal muscle microRNA expression at rest and after an anabolic stimulus of resistance exercise and essential amino acids. Am J Physiol Endocrinol Metab 295(6):E1333–E1340
Will K, Kalbe C, Kuzinski J, Losel D, Viergutz T, Palin MF et al (2012) Effects of leptin and adiponectin on proliferation and protein metabolism of porcine myoblasts. Histochem Cell Biol 138(2):271–287
Anderson RM, Weindruch R (2010) Metabolic reprogramming, caloric restriction and aging. Trends Endocrinol Metab 21(3):134–141
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Morioka, T., Mori, K., Motoyama, K., Emoto, M. (2016). Ectopic Fat Accumulation and Glucose Homeostasis: Role of Leptin in Glucose and Lipid Metabolism and Mass Maintenance in Skeletal Muscle. In: Inaba, M. (eds) Musculoskeletal Disease Associated with Diabetes Mellitus. Springer, Tokyo. https://doi.org/10.1007/978-4-431-55720-3_14
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DOI: https://doi.org/10.1007/978-4-431-55720-3_14
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