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The Lipidomic Analyses in Low and Highly Aggressive Ovarian Cancer Cell Lines

  • Original Article
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Lipids

Abstract

Despite huge advances in the research of epithelial ovarian cancer (EOC), it remains the most lethal gynecological malignancy. Peritoneal tumor cell dissemination with cell survival and drug-resistance to taxane and platinum-based chemotherapy are two of the major challenges of EOC treatment. We have generated highly aggressive EOC cell lines (ID8-P1 lines or P1) from ID8-P0 (without in vivo passage, or P0) through in vivo passage in mice. We conducted lipidomic analyses in cells from ID8-P0 versus three ID8-P1 cell lines using ultra-high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. A total of 16 classes of lipids (149 individual lipids) were analyzed and compared between P0 and P1 cells. In addition to overall lipid profiles in EOC cells, we had several novel observations. Several classes and species of lipids have been identified to be differentially present in P0 versus P1 cells, which are potentially involved in the acquired aggressiveness of P1 cells. Triacylglycerols (TAG) were dramatically increased under detachment stress in EOC cells. Since survival of EOC cells under detachment is one of the major obstacles for EOC treatment, further studies identifying the molecular mechanisms controlling TAG increase may lead to new treatment modalities for EOC.

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Abbreviations

CB:

Cerebroside

Cer:

Ceramide

CH3CN:

Acetonitrile

CHCl3 :

Chloroform

EOC:

Epithelial ovarian cancer

ESI-MS:

Electrospray ionization-mass spectrometry

IPA:

Isopropanol

lysoPtdOH:

Lysophosphatidic acid

lysoPtdCho:

Lysophosphatidylcholine

lysoPtdEtn:

Lysophosphatidylethanolamine

lysoPtdGro:

Lysophosphatidylglycerol

lysoPtdIns:

Lysophosphatidylinositol

lysoPtdSer:

Lysophosphatidylserine

MeOH:

Methanol

MRM:

Multiple reaction monitoring

PtdOH:

Phosphatidic acid

PtdCho:

Phosphatidylcholine

PtdEtn:

Phosphatidylethanolamine

PtdGro:

Phosphatidylglycerol

PtdIns:

Phosphatidylinositol

PtdSer:

Phosphatidylserine

RT:

Retention time

CerPCho:

Sphingomyelin

TAG:

Triacylglycerol(s)

UHPLC:

Ultra-high-performance liquid chromatography

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Acknowledgments

We would like to thank Dr. Jill Reiter and Mr. Kevin J. McClelland for editing the manuscript.

Funding

This work was supported in part by the Science and Technology Program of Beijing Municipality (No. Z 131100005213009), National Natural Science Foundation of China (Grant No. 21321003), the National Institutes of Health (RO1 155145 to YX); and the Mary Fendrich-Hulman Charitable Trust Fund to YX.

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Authors and Affiliations

  1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Sciences, Beijing, 100190, China

    Zhenwen Zhao

  2. Department of Obstetrics and Gynecology, Indiana University School of Medicine, 975 W. Walnut St. IB355A, Indianapolis, IN, 46202, USA

    Qingchun Cai & Yan Xu

Authors
  1. Zhenwen Zhao
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  2. Qingchun Cai
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  3. Yan Xu
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Corresponding authors

Correspondence to Zhenwen Zhao or Yan Xu.

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The authors declare no competing financial interest.

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Zhao, Z., Cai, Q. & Xu, Y. The Lipidomic Analyses in Low and Highly Aggressive Ovarian Cancer Cell Lines. Lipids 51, 179–187 (2016). https://doi.org/10.1007/s11745-015-4108-7

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  • DOI: https://doi.org/10.1007/s11745-015-4108-7

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