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Oxidative Modification and Poor Protective Activity of HDL on LDL Oxidation in Thalassemia

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Lipids

Abstract

Oxidative modification of low-density lipoprotein (LDL) has been reported in thalassemia, which is a consequence of oxidative stress. However, the levels of oxidized high-density lipoprotein (HDL) in thalassemia have not been evaluated and it is unclear whether HDL oxidation may be linked to LDL oxidation. In this study, the levels of total cholesterol, iron, protein, conjugated diene (CD), lipid hydroperoxide (LOOH), and thiobarbituric acid reactive substances (TBARs) were determined in HDL from healthy volunteers and patients with β-thalassemia intermedia with hemoglobin E (β-thal/Hb E). The protective activity of thalassemic HDL on LDL oxidation was also investigated. The iron content of HDL2 and HDL3 from β-thal/HbE patients was higher while the cholesterol content was lower than those in healthy volunteers. Thalassemic HDL2 and HDL3 had increased levels of lipid peroxidation markers i.e., conjugated diene, LOOH, and TBARs. Thalassemic HDL had lower peroxidase activity than control HDL and was unable to protect LDL from oxidation induced by CuSO4. Our findings highlight the oxidative modification and poor protective activity of thalassemic HDL on LDL oxidation which may contribute to cardiovascular complications in thalassemia.

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Abbreviations

CD:

Conjugated diene

HDL:

High-density lipoprotein

LDL:

Low-density lipoprotein

LOOH:

Lipid hydroperoxide

OD:

Optical density

TBARs:

Thiobarbituric acid reactive substances

β-thal/Hb E:

β-Thalassemia with hemoglobin E

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Acknowledgments

The authors would like to thank Prof. Dr. Suthat Fucharoen, Prof. Dr. Pensri Pootrakul from Thalassemia Research Center, Institute of Science and Technology for Research and Development, Mahidol University; Dr. Wanchai Wanachivanawin from Department of Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University for screening and care of thalassemic patients. We also thank to all staffs and blood donors of Blood Bank Ramathibodi Hospital.

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Science, Mahidol University, Rama 6 Rd, Bangkok, 10400, Thailand

    Supeenun Unchern, Yupin Sanvarinda, Pansakorn Tanratana & Nathawut Sibmooh

  2. Laboratory of Immunology, Chulabhorn Research Institute, Vibhavadee-Rangsit Highway, Laksi, Bangkok, 10210, Thailand

    Narumon Laohareungpanya

  3. Office for Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

    Kovit Pattanapanyasat

  4. Faculty of Chinese Medicine, Huachiewchalermprakiet University, 18/18, Bangna-Trad Road, k.m. 18, Bangplee, Samut Prakarn, 10540, Thailand

    Udom Chantharaksri

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  1. Supeenun Unchern
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  2. Narumon Laohareungpanya
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Correspondence to Nathawut Sibmooh.

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Unchern, S., Laohareungpanya, N., Sanvarinda, Y. et al. Oxidative Modification and Poor Protective Activity of HDL on LDL Oxidation in Thalassemia. Lipids 45, 627–633 (2010). https://doi.org/10.1007/s11745-010-3435-y

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  • DOI: https://doi.org/10.1007/s11745-010-3435-y

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