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Identification and Characterization of Hamster Stearoyl-CoA Desaturase Isoforms

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Lipids

Abstract

Stearoyl-CoA desaturase (SCD) catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. It plays a key role in lipid metabolism and energy expenditure in mammals. In mice, four SCD isoforms (SCD1–4) have been identified. Here we report the identification of cDNA sequences corresponding to SCD1, SCD2 and SCD3 of golden hamster. The deduced amino acid sequences of these hamster SCD (hmSCD) isoforms display a high degree of homologies to their mouse counterparts (mouse SCD). Polyclonal antibodies specific to rodent SCDs detected proteins of predicted size in the human embryonic kidney 293 cells transfected with hmSCD cDNAs. Microsome fractions prepared from these cells also displayed increased SCD activity versus cells transfected with vector alone. Real-time reverse transcription-polymerase chain reaction analysis revealed the highest expression of hmSCD1 in liver and adipose tissue, while the highest hmSCD2 expression was detected in the brain. Very low levels of hmSCD3 mRNA can be detected in the tissues tested. This report is the first description of three SCD isoforms in the hamster and will provide useful tools in the further study of fatty acids metabolism in this species.

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Abbreviations

Bp:

Base pair(s)

cDNA:

DNA complementary to RNA

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

HDL:

High density lipoprotein

kb:

Kilobase(s) or 1,000 bp

PCR:

Polymerase chain reaction

RT:

Reverse transcription

SCD:

Stearoyl-CoA desaturase

TLC:

Thin-layer chromatography

VLDL:

Very low density lipoprotein

CDS:

Coding sequence

References

  1. Enoch HG, Catala A, Strittmatter P (1976) Mechanism of rat liver microsomal stearyl-CoA desaturase. Studies of the substrate specificity, enzyme-substrate interactions, and the function of lipid. J Biol Chem 251:5095–5103

    PubMed  CAS  Google Scholar 

  2. Heinemann FS, Korza G, Ozols J (2003) A plasminogen-like protein selectively degrades stearoyl-CoA desaturase in liver microsomes. J Biol Chem 278:42966–42975

    Article  PubMed  CAS  Google Scholar 

  3. Ntambi JM, Miyazaki M (2004) Regulation of stearoyl-CoA desaturases and role in metabolism. Prog Lipid Res 43:91–104

    Article  PubMed  CAS  Google Scholar 

  4. Miyazaki M, Kim Y, Gray-Keller MP, Attie AD, Ntambi JM (2000) The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase 1. J Biol Chem 275:30132–30138

    Article  PubMed  CAS  Google Scholar 

  5. Cohen P, Miyazaki M, Socci ND, Hagge-Greenberg A, Liedtke W, Soukas AA, Sharma R, Hudgins LC, Ntambi JM, Friedman JM (2002) Role for stearoyl-CoA desaturase-1 in leptin-mediated weight loss. Science 297:240–243

    Article  PubMed  CAS  Google Scholar 

  6. Attie AD, Krauss RM, Gray-Keller MP, Brownlie A, Miyazaki M, Kastelein JJ, Lusis AJ, Stalenhoef AF, Stoehr JP, Hayden MR, Ntambi JM (2002) Relationship between stearoyl-CoA desaturase activity and plasma triglycerides in human and mouse hypertriglyceridemia. J Lipid Res 43:1899–1907

    Article  PubMed  CAS  Google Scholar 

  7. Sun Y, Hao M, Luo Y, Liang CP, Silver DL, Cheng C, Maxfield FR, Tall AR (2003) Stearoyl-CoA desaturase inhibits ATP-binding cassette transporter A1-mediated cholesterol efflux and modulates membrane domain structure. J Biol Chem 278:5813–5820

    Article  PubMed  CAS  Google Scholar 

  8. Wang Y, Oram J (2005) Unsaturated fatty acids phosphorylate and destabilize ABCA1 through a phospholipase D2 pathway. J Biol Chem 280:35896–35903

    Article  PubMed  CAS  Google Scholar 

  9. Senanayake S, Brownrigg LM, Panicker V, Croft KD, Joyce DA, Steer JH, Puddey IB, Yeap BB (2007) Monocyte-derived macrophages from men and women with type 2 diabetes mellitus differ in fatty acid composition compared with non-diabetic controls. Diabetes Res Clin Pract 75:292

    Article  PubMed  CAS  Google Scholar 

  10. Miyazaki M, Jacobson MJ, Man W, Cohen P, Asilmaz E, Friedman JM, Ntambi JM (2003) Identification and characterization of murine SCD4, a novel heart-specific stearoyl-CoA desaturase isoform regulated by leptin and dietary factors. J Biol Chem 278:33904–33911

    Article  PubMed  CAS  Google Scholar 

  11. Zheng Y, Prouty SM, Harmon A, Sundberg JP, Stenn KS, Parimoo S (2001) Scd3—a novel gene of the stearoyl-CoA desaturase family with restricted expression in skin. Genomics 71:182–191

    Article  PubMed  CAS  Google Scholar 

  12. Zhang L, Ge L, Parimoo S, Stenn K, Prouty SM (1999) Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites. Biochem J 340:255–264

    Article  PubMed  CAS  Google Scholar 

  13. Wang J, Yu L, Schmidt RE, Su C, Huang X, Gould K, Cao G (2005) Characterization of HSCD5, a novel human stearoyl-CoA desaturase unique to primates. Biochem Biophy Res Commun 332:735–742

    Article  CAS  Google Scholar 

  14. Beiraghi S, Zhou M, Talmadge CB, Went-Sumegi N, Davis JR, Huang D, Saal H, Seemayer TA, Sumegi J (2003) Identification and characterization of a novel gene disrupted by a pericentric inversion inv(4)(p13.1q21.1) in a family with cleft lip. Gene 309:11–21

    Article  PubMed  CAS  Google Scholar 

  15. Spady DK, Dietschy JM (1983) Sterol synthesis in vivo in 18 tissues of the squirrel monkey, guinea pig, rabbit, hamster, and rat. J Lipid Res 24:303–315

    PubMed  CAS  Google Scholar 

  16. Otto J, Ordovas JM, Smith D, van Dongen D, Nicolosi RJ, Schaefer EJ (1995) Lovastatin inhibits diet induced atherosclerosis in F1B golden Syrian hamsters. Atherosclerosis 114:19–31

    Article  PubMed  CAS  Google Scholar 

  17. Nistor A, Bulla A, Fillip DA, Radu A (1987) The hyperlipidemic hamsters as a model of experimental atherosclerosis. Atherosclerosis 68:159–173

    Article  PubMed  CAS  Google Scholar 

  18. Livak KA, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔC t method. Methods 25:402–408

    Article  PubMed  CAS  Google Scholar 

  19. Ideta R, Seki T, Adachi K (1995) Sequence analysis and characterization of FAR-17c, an androgen-dependent gene in the flank organs of hamsters. J Dermatol Sci 9:94–106

    Article  PubMed  CAS  Google Scholar 

  20. Trautwein EA, Liang J, Hayes KC (1993) Cholesterol gallstone induction in hamsters reflects strain differences in plasma lipoproteins and bile acid profiles. Lipids 28:305–312

    Article  PubMed  CAS  Google Scholar 

  21. Miyazaki M, Bruggink SM, Ntambi JM (2006) Identification of mouse palmitoyl-coenzyme A {Delta}9-desaturase. J Lipid Res 47:700–704

    Article  PubMed  CAS  Google Scholar 

  22. Lengi AJ, Corl BA (2007) Identification and characterization of a novel bovine stearoyl-CoA desaturase isoform with homology to human SCD5. Lipids 42:499–508

    Article  PubMed  CAS  Google Scholar 

  23. Miyazaki M, Dobrzyn A, Elias PM, Ntambi JM (2005) Stearoyl-CoA desaturase-2 gene expression is required for lipid synthesis during early skin and liver development. Proc Natl Acad Sci USA 102:12501–12506

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

We would like to thank Liyun Ding and Julia Cao for technical assistance and Dr Marian Mosior for helpful discussions.

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Authors and Affiliations

  1. Cardiovascular and Diabetes Drug Discovery, Lilly Research Labs, Lilly Corporate Center, DC 0434, Indianapolis, IN, 46285, USA

    Jian Wang, Lan Yu, Yunling Gao, James P. Schrementi, Regina K. Porter, Chen-Shian Suen, Guoqing Cao, James S. Bean & Raymond F. Kauffman

  2. Integrative Biology, Lilly Research Labs, DC 0343, Indianapolis, IN, 46285, USA

    He Wang & Yuewei Qian

  3. Discovery Chemistry, Lilly Research Labs, DC 0343, Indianapolis, IN, 46285, USA

    David A. Yurek & Mingshang Kuo

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  1. Jian Wang
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  2. Lan Yu
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  3. He Wang
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Correspondence to Jian Wang.

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Wang, J., Yu, L., Wang, H. et al. Identification and Characterization of Hamster Stearoyl-CoA Desaturase Isoforms. Lipids 43, 197–205 (2008). https://doi.org/10.1007/s11745-007-3139-0

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  • DOI: https://doi.org/10.1007/s11745-007-3139-0

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