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Regulation by carbohydrate and clofibric acid of palmitoyl-CoA chain elongation in the liver of rats

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Lipids

Abstract

Regulation of palmitoyl-CoA chain elongation (PCE) and its contribution to oleic acid formation were investigated in rat liver in comparison with stearoyl-CoA desaturase (SCD). Hepatic PCE activity was induced by the administration of 20% wt/vol glucose or fructose in the drinking water of normal rats. In streptozotocin-induced diabetic rats, the activities of both PCE and SCD were suppressed, and fructose, but not glucose, feeding caused an increase in the activities of both enzymes. Treatment of normal rats with clofibric acid in combination with carbohydrate further increased PCE, but not SCD, acitivity. FA analysis of hepatic lipids revealed that the proportion of oleic acid (18∶1n−9) increased upon administration of carbohydrate or clofibric acid. The treatment of rats with clofibric acid in combination with carbohydrate greatly increased the proportion of 18∶1n−9. A significant correlation was observed between PCE activity and the hepatic proportion of 18∶1n−9 (r 2=0.874, Pº0.01), whereas the relationship between SCD activity and the proportion of 18∶1n−9 was not significant (r 2=0.552, P>0.05). Taken together, these results suggest that carbohydrate induces PCE as well as SCD activity to increase the hepatic 18∶1 content in rat liver, and the increased PCE activity seems to be responsible for the further increase in 18∶1n−9 when carbohydrate is administered in combination with clofibric acid.

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Abbreviations

16:0:

palmitic acid

16∶1n−7:

palmitoleic acid

18∶0:

stearic acid

18∶1nv-9:

oleic acid

18∶1n−7:

vaccenic acid

1-acyl-GPC:

1-acylglycerophosphocholine

clofibric acid:

2-(p-chlorophenoxy)-2-methylpropionic acid

PCE:

palmitoyl-CoA chain elongation

PPARα:

peroxisome proliferatoractivated receptor α

PPRE:

peroxisome proliferator responsive element

SCD:

stearoyl-CoA desaturase

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Josai University, Keyakidai 1-1, Sakado, 350-0295, Saitama, Japan

    Naomi Kudo, Tomoaki Toyama, Atsushi Mitsumoto & Yoichi Kawashima

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  1. Naomi Kudo
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  2. Tomoaki Toyama
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  3. Atsushi Mitsumoto
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  4. Yoichi Kawashima
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Correspondence to Naomi Kudo.

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FA are designated by the number of carbon atoms and double bonds

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Kudo, N., Toyama, T., Mitsumoto, A. et al. Regulation by carbohydrate and clofibric acid of palmitoyl-CoA chain elongation in the liver of rats. Lipids 38, 531–537 (2003). https://doi.org/10.1007/s11745-003-1338-8

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  • DOI: https://doi.org/10.1007/s11745-003-1338-8

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