Skip to main content
SpringerLink
Log in

Effect of starvation on lipid metabolism and stability of DHA content of lipids in horse mackerel (Trachurus japonicus) tissues

  • Articles
  • Published:
Lipids

Abstract

For the purpose of characterizing the effect of starvation on 22∶6n−3 (DHA) content in marine fish tissues, horse mackerel (Trachurus japonicus) were reared in a tank containing filtered, sterilized seawater under nonfeeding conditions for 107 d (survival rate of the fish was 96.5%). The crude total lipids (TL) of ordinary dorsal muscle, dorsal skin, and viscera of the starved individuals were separated into classes on silicic acid columns, and the constituents of the TL were quantified by gravimetric recovery from column chromatography. The TL, initially>85% TAG in dorsal muscle, and even more in skin lipids, decreased dramatically within the first 44 d of starvation, and then decreased more gradually during the remainder of the test period, whereas the visceral TL decreased more slowly. The percentages of both saturated and monoenoic FA in the muscle TL also decreased somewhat, but those of DHA increased significantly in muscle during the test periods. Decreases in PE and PC initially were much smaller than TAG, but DHA levels remained high in both PE and PC. These findings indicate that all of the FA in the depot lipids of horse mackerel tissues are easily metabolized for energy production during starvation, but DHA in muscle lipids of the starved fish was maintained at a consistently high level, indicating that starvation did not affect DHA stability in phospholipids. The findings suggest that preservation of DHA in cell membrane lipid PE and PC is necessary for self-protection functions in starving fish.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

PL:

phospholipid

SE:

steryl esters

ST:

sterols

TL:

crude total lipids

References

  1. Osako, K., Yamaguchi, A., Kurokawa, T., Kuwahara, K., Saito, H., and Nozaki, Y. (2003) Seasonal Variation in Docosahexaenoic Acid Content in Horse Mackerel Caught in the East China Sea, Fish. Sci. 69, 589–596.

    Article  CAS  Google Scholar 

  2. Inui, Y., and Oshima, Y. (1966) Effect of Starvation on Metabolism and Chemical Composition of Eels, Nippon Suisan Gakkaishi 32, 492–501.

    CAS  Google Scholar 

  3. Satoh, S., Takeuchi, T., and Watanabe, T. (1984) Effects of Starvation and Environmental Temperature on Proximate and Fatty Acid Composition of Tilapia nilotica, Nippon Suisan Gakkaishi 50, 79–84.

    CAS  Google Scholar 

  4. Shimma, Y., Ichimura, H., and Shibata, N. (1976) Effect of Starvation on Body Weight, Lipid Contents, and Plasma Constituents of Maturing Rainbow Trout, Nippon Suisan Gakkaishi 42, 83–89.

    CAS  Google Scholar 

  5. Kaneko, T., Takeuchi, M., Ishii, S., Higashi, H., and Kikuchi, T. (1966) Effect of Dietary Lipids on Fish under Cultivation—IV. Changes of Fatty Acid Composition in Flesh Lipids of Rainbow Trout on Non-feeding, Nippon Suisan Gakkaishi 33, 56–58

    Google Scholar 

  6. Takeuchi, T., and Watanabe, T. (1982) Effects of Starvation and Environmental Temperature on Proximate and Fatty Acid Composition of Carp and Rainbow Trout, Nippon Suisan Gakkaishi 48, 1307–1316.

    CAS  Google Scholar 

  7. Talesnik, J., and Hsia, J.C. (1982) Coronary Flow Reactions to Arachidonic Acid Are Inhibited by Docosahexaenoic Acid, Eur. J. Pharmacol. 80, 255–258.

    Article  PubMed  CAS  Google Scholar 

  8. Ackman, R.G. (1988) Concerns for Utilization of Marine Lipids and Oils, Food Technol. 42, 151–155.

    CAS  Google Scholar 

  9. Kinsella, J.E. (1988) Food Lipids and Fatty Acids, Food Technol. 42, 124–145.

    CAS  Google Scholar 

  10. Hubert, J., and Sustr, V. (2001) The Effect of Starvation on the Metabolic Rate and Microanatomy of Galumna elimata (Acari: Oribatida), Eur. J. Entomol. 98, 265–275.

    Google Scholar 

  11. Murata, H., and Higashi, T. (1980) Selective Utilization of Fatty Acids as Energy Source in Carp, Nippon Suisan Gakkaishi 46, 1333–1338.

    CAS  Google Scholar 

  12. Takeuchi, T., Watanabe, T., and Satoh, S. (1987) Development of Less-Polluting Diets for Practical Fish Culture. II. Changes in Proximate and Fatty Acid Compositions of Carp Fed Low Protein-High Energy Diets due to Starvation During Winter, Nippon Suisan Gakkaishi 53, 1425–1429.

    CAS  Google Scholar 

  13. Yamada, S., Tanaka, Y., Sameshima, M., and Furuichi, M. (1994) Effects of Starvation and Feeding on Tissue N α-Acetylhistidine Levels in Nile Tilapia Oreochromis niloticus, Comp. Biochem. Physiol., Part A, Physiol. 109A, 277–283.

    Article  CAS  Google Scholar 

  14. Hayashi, K., and Takagi, T. (1977) Lipid Metabolism in Fish II. Changes of Lipids and Fatty Acids in the Liver of Puffer, Fugu vermiculare porphyreum, During Starvation, Bull. Fac. Fish Hokkaido Univ. 28, 193–201.

    CAS  Google Scholar 

  15. Takeda, S., Honda, H., and Kiyono, M. (1991) Effect of Feeding on Nitrogen Excretion of Japanese Flounder Paralichthys olivaceus, Nippon Suisan Gakkaishi 57, 2059–2064.

    Google Scholar 

  16. Folch, J., Lees, M., and Sloane Stanley, G.H. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  17. Murata, H., and Higashi, T. (1979) Studies on the Metabolism of Fatty Acid in Fish—IV. Rate of Fatty Acid Decrease Based on β-Oxidation in Carp Muscle Mitochondria, Nippon Suisan Gakkaishi 45, 211–217.

    CAS  Google Scholar 

  18. Murata, H. (1979) Studies on the Metabolism of Fatty Acid in Fish—V. β-Oxidation of 22∶6 Acid in Fish Liver and Dark Muscle Mitochondria, Nippon Suisan Gakkaishi 45, 379–383.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Nagasaki Prefectural Institute of Fisheries, Taira, 851-2213, Nagasaki, Japan

    Kazufumi Osako & Koichi Kuwahara

  2. National Research Institute of Fisheries Science, Yokohama, 236-8648, Kanagawa, Japan

    Hiroaki Saito

  3. Faculty of Fisheries, Nagasaki University, 852-8521, Nagasaki, Japan

    Mohammed Anwar Hossain & Yukinori Nozaki

Authors
  1. Kazufumi Osako
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Koichi Kuwahara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hiroaki Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mohammed Anwar Hossain
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yukinori Nozaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kazufumi Osako.

About this article

Cite this article

Osako, K., Kuwahara, K., Saito, H. et al. Effect of starvation on lipid metabolism and stability of DHA content of lipids in horse mackerel (Trachurus japonicus) tissues. Lipids 38, 1263–1267 (2003). https://doi.org/10.1007/s11745-003-1187-5

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11745-003-1187-5

Keywords

Search

Navigation