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Trans-18∶1 isomers in rat milk fat as effective biomarkers for the determination of individual isomeric trans-18∶1 acids in the dams' diet

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Lipids

Abstract

Female rats were fed a diet containing by weight 10% partially hydrogenated sunflower oil, 2% sunflower oil, and 1% rapeseed oil during gestation and lactation. The trans-18∶1 isomer profile of the fat supplement was (in % of total trans 18∶1 acids in the fat supplement): Δ4, 0.5; Δ5, 1.0;Δ6–Δ8, 18∶0; Δ9 (elaidic), 13.5; Δ10, 22.2;Δ11 (vaccenic), 16.0; Δ12, 11.3; Δ13–Δ14, 12.8; Δ15, 2.5; and Δ16, 2.2 (total trans 18∶1 acids in the fat supplement: 40.6%). The cis 18∶1 isomer profile was (in % of total cis-18∶1 isomers):Δ6, Δ8, 2.1; Δ9 (oleics), 70.9; Δ10, 6.1; Δ11, 8.3; Δ12, 4.0; Δ13, 2.8; Δ14, 4.6, and Δ15, 1.2 (total cis-18∶1 acids in the fat supplement: 32.6%). Suckling rats from four litters were sacrificed at day 17 or 18 after birth, and their stomach content (milk) was analyzed. The trans-18∶1 isomer profile of milk was (relative proportions, in % of total): Δ4, 0.3; Δ5, 1.1; Δ6–Δ8, 16.8; Δ9, 15.3; Δ10, 22.0; Δ11, 16.7; Δ12, 11.8; Δ13–14, 11.8; Δ15, 2.5, and Δ16, 1.9 (total trans 18∶1 acids in milk: %). That of cis-18∶1 isomers was (proportions in % relative to total cis-18∶1 isomers): Δ6–Δ8, 4.7; Δ9, 72.5; Δ10, 4.0; Δ11, 8.0; Δ12, 7.1; Δ13, 1.9; Δ14, 1.0, and Δ15, 0.7 (total cis-18∶1 acids in milk: %). These results demonstrate that all isomeric acids, independent of the geometry and the position of the ethylenic bond, are incorporated into milk lipids. With regard to trans-18∶1 isomers, the distribution profile in milk is identical to that in the dams' diet, i.e., there is no discrimination against any positional isomer between their ingestiona nd their deposition into milk lipids. As a consequence, this study indicates that the trans-18∶1 isomer profile of milk reflects that in the dams' diet and supports our earlier hypothesis that the profile of trans-18∶1 isomers in milk can be used to deduce the relative contribution of ruminant fats and partially hydrogenated oils in the diet ot the total intake of trans-18∶1 isomers. On the other hand, the cis-18∶1 isomer profile in milk shows significant differences when compared to that in the dams' diet. Surprisingly, there are no major differences for the cis-Δ9 (oleic) and the cis-Δ11 (asclepic) isomers, which can be synthesized by the mother. However, there seems to be a significant positive selectivity for the group cis-Δ6–Δ8, and for the cis-Δ12 isomer, whereas a negative selectivity occurs for the Δ10 and Δ13 to Δ15 cis isomers.

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Abbreviations

DCF:

2′, 7′-dichlorofluorescein

PHSO:

partially hydrogenated sunflower oil

PHVO:

partially hydrogenated vegetable oil

References

  1. Wolff, R.L., Precht, D., and Molkentin, J. (1998) Trans-18∶1 Acid Content and Profile in Human Milk Lipids. Critical Survey of Data in Connection with Analytical Methods, J. Am. Oil Chem. Soc. 75, 661–671.

    CAS  Google Scholar 

  2. Wolff, R.L. (1995) Content and Distribution of trans-18∶1 Acids in Ruminant Milk and Meat Fats. Their Importance in European Diets and Their Effect on Human Milk, J. Am. Oil Chem. Soc. 72, 259–272.

    CAS  Google Scholar 

  3. Chardigny, J.M., Wolff, R.L., Mager, E., Sébédio, J.-L., Martine, L., and Juanéda, P. (1995) Trans Mono-and Polyunsaturated Fatty Acids in Human Milk, Eur. J. Clin. Nutr. 49, 523–531.

    PubMed  CAS  Google Scholar 

  4. Precht, D., and Molkentin, J. (1999) C18∶1, C18∶2 and C18∶3 trans and cis Fatty Acid Isomers Including Conjugated cis Δ9,trans Δ11 Linoleic Acid (CLA) as Well as Total Fat Composition of German Human Milk Lipids, Nahrung 43, 233–244.

    Article  PubMed  CAS  Google Scholar 

  5. Chen, Z.Y., Pelletier, G., Hollywood, R., and Ratanyake, W.M.N. (1995) Trans Fatty Isomers in Canadian Human Milk, Lipids 30, 15–21.

    PubMed  CAS  Google Scholar 

  6. Chen, Z.Y., Ratnayake, W.M.N., Fortier, L., and Cunnane, S.C. (1995) Similar Distribution of trans Fatty Acid Isomers in Partially Hydrogenated Vegetable Oils and Adipose Tissue of Canadians, Can. J. Physiol. Pharmacol. 73, 718–723.

    PubMed  CAS  Google Scholar 

  7. Boué, C., Combe, N., Billeaud, C., Mignerot, C., Entressangles, B., Théry, G., Brun, J.L., Dallay, D., and Leng, J.J. (2000) Trans Fatty Acids in Adipose Tissue of French Women in Relation to Their Dietary Sources, Lipids 35, 561–566.

    Article  PubMed  Google Scholar 

  8. Wolff, R.L., Combe, N.A., Destaillats, F., Boué, C., Precht, D., Molkentin, J., and Entressangles, B. (2000) Follow-Up of the Δ4 to Δ16 trans-18∶1 Isomer Profile and Content in French Processed Foods Containing Partially Hydrogenated Vegetable Oils During the Period 1995–1999. Analytical and Nutritional Implications, Lipids 35, 815–825.

    Article  PubMed  CAS  Google Scholar 

  9. Wolff, R.L., Precht, D., and Molkentin, J. (1998) Occurrence and Distribution Profile of trans-18∶1 Acids in Edible Fats of Natural Origin, in Trans Fatty Acids in Human Nutrition (Sébédio, J.-L., and Christie, W.W., eds.), pp. 1–33, Oily Press, Dundee.

    Google Scholar 

  10. Wolff, R.L., and Entressangles, B. (1994) Steady-State Fluorescence Polarization Study of Structurally Defined Phospholipids from Liver Mitochondria of Rats Fed Elaidic Acid, Biochim. Biophys. Acta 1211, 198–206.

    PubMed  CAS  Google Scholar 

  11. Wolff, R.L. (1995) Structural Importance of the cis-5 Ethylenic Bond in the Endogenous Desaturation Product of Dietary Elaidic Acid, cis-5, trans-9 18∶2 Acid, for the Acylation of Rat Mitochondria Phosphatidylinositol, Lipids 30, 893–898.

    PubMed  CAS  Google Scholar 

  12. Santora, J.E., Palmquist, D.L., and Roehrig, K.L. (2000) Trans-Vaccenic [sic] Acid Is Desaturated to Conjugated Linoleic Acid in Mice, J. Nutr. 138, 208–215.

    Google Scholar 

  13. Ip, C., Banni, S., Angioni, E., Carta, G., McGinley, J., Thompson, H.J., Barbano, D., and Bauman, D. (1999) Conjugated Linoleic Acid-Enriched Butter Fat Alters Mammary Gland Morphogenesis and Reduces Cancer Risk in Rats, J. Nutr. 129, 2135–2142.

    PubMed  CAS  Google Scholar 

  14. Wolff, R.L., and Bayard, C.C. (1995) Improvement in the Resolution of Individual trans-18∶1 Isomers by Capillary Gas-Liquid Chromatography: Use of a 100-m CP-Sil 88 Column, J. Am. Oil Chem. Soc. 72, 1197–1201.

    CAS  Google Scholar 

  15. Folch, J., Lees, M., and Sloane-Stanley, G.M. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  16. Wolff, R.L., Bayard, C.C., and Fabien, R.J. (1995) Evaluation of Sequential Methods for the Determination of Butterfat Fatty Acid Composition with Emphasis on trans-18∶1 Acids. Application to the Study of Seasonal Variations in French Butters, J. Am. Oil Chem. Soc. 72, 1471–1483.

    CAS  Google Scholar 

  17. Wood, R. (1979) Distribution of Dietary Geometrical and Positional Isomers in Brain, Heart, Kidney, Liver, Lung, Muscle, Spleen: Adipose and Hepatoma, in Geometrical and Positional Fatty Acid Isomers (Emken, E.A., and Dutton, H.J., eds.), pp. 213–281, American Oil Chemists' Society, Champaign.

    Google Scholar 

  18. Reichwald-Hacker, I., Grosse-Oetringhaus, S., Kiewitt, I., and Mukherjee, K.D. (1979) Incorporation of Positional Isomers of cis-and trans-Octadecenoic Acids into Acyl Moieties of Rat Tissue, Biochim. Biophys. Acta 575, 327–334.

    PubMed  CAS  Google Scholar 

  19. Høy, C.-E., and Hølmer, G. (1979) Incorporation of cis-and trans-Octadecenoic Acids into the Membranes of Rat Liver Mitochondria, Lipids 14, 727–733.

    PubMed  Google Scholar 

  20. Ohlrogge, J.B., Emken, E.A., and Gulley, R.M. (1981) Human Tissue Lipids: Occurrence of Fatty Acid Isomers from Dietary Hydrogenated Oils, J. Lipid Res. 22, 955–960.

    PubMed  CAS  Google Scholar 

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  1. Unité de Nutrition Lipidique, INRA, 17, rue Sully, B.P. 86510, 21065, Dijon cedex, France

    Robert L. Wolff

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  1. Robert L. Wolff
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Correspondence to Robert L. Wolff.

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Dr. Robert L. Wolff Robert Wolff passed away at the age of 53 on the 10th of November, 2002. His know-how in the field of lipids was recognized internationally. He had the ability to lead his research projects in both the animal and vegetal worlds. His scientific achievement, more than 100 publications to his name in the field of trans fatty acids, made him highly esteemed by his colleagues. He was Conference Master at Bordeaux 1 University (France) up until 2001, at which time he joined the Nutritional Lipid Unit in I.N.R.A., Dijon (France). His mission there was to develop a research program on plasmalogens and their role in brain and muscle function, for which his analytical and biochemical skills were a guarantee of success. Unfortunately, his state of health did not allow him to complete this project. This publication is his final one.

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Wolff, R.L. Trans-18∶1 isomers in rat milk fat as effective biomarkers for the determination of individual isomeric trans-18∶1 acids in the dams' diet. Lipids 38, 1143–1148 (2003). https://doi.org/10.1007/s11745-003-1172-z

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  • DOI: https://doi.org/10.1007/s11745-003-1172-z

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