Abstract
The aim of the present study was to elucidate whether the qualitative compositiion of dietary fat influences plasma leptin and adipose tissue ob gene expression differentially. Two high-fat diets and a diet rich in carbohydrate were each administered both ad libitum and with a 25% energy restriction. The high-fat diets contained 58 energy percent as either monounsaturated FA (MUFA) or saturated FA (SAFA), whereas the carbohydrate-rich diet (CH) contained 7 energy percent as fat. We aimed at obtaining the same final weight for the animals in the ad libitum group as in the energy-restricted groups. This goal was reached at he same time (days 22–24) for all groups except for the ad libitum animals fed on saturated fat (day 36). The plasma leptin concentrations on ad libitum CH and MUFA diets did not differ significantly (24.3 ± 2.1 and 34.7 ± 6.7 ng/mL, respectively) whereas the saturated fat diet caused a lower concentration (13.9 ± 1.9 ng/mL; P < 0.05). Interestingly, no differences in plasma leptin levels between groups were seen in the energy-restricted groups (mean 8.0 + 1.0 ng/mL). The type of diet did not alter the ob gene expression in intraabdominal white adipose tissue; however, a lower expression level was found in the energy-restricted groups. The percentage of body fat in the three ad libitum fed groups did not differ (23 ± 1%). Thus, short-term administration of a diet rich in SAFA suppresses circulating leptin levels without altering the adipose tissue ob gene expression. This indicates that saturated fat may alter protein handling by adipose tissue or the whole body clearance of leptin.
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Abbreviations
- CH:
-
carbohydrate-rich (control) diet
- MUFA:
-
diet rich in monounsaturated FA
- SAFA:
-
diet rich in saturated FA
References
Zhang, Y., Proenca, R., Maffei, M., Barone, M., Leopold, L., and Friedman, J.M. (1994) Positional Cloning of the Mouse Obese Gene and Its Human Homologue, Nature 372, 425–432.
Maffei, M., Halaas, J., Ravussin, E., Pratley, R.E., Lee, G.H., Zhang, Y., Fei, H., Kim, S., Lallone, R., Ranganathan, S., et al. (1995) Leptin Levels in Human and Rodent: Measurement of Plasma Leptin and ob RNA in Obese and Weight-Reduced Subjects, Nat. Med. 1, 1155–1161.
Frederich, R.C., Hamann, A., Anderson, S., Lollmann, B., Lowell, B.B., and Flier, J.S. (1995) Leptin Levels Reflect Body Lipid Content in Mice: Evidence for Diet Induced Resistance to Leptin Action, Nat. Med. 1, 1311–1313.
Masuzaki, H., Ogawa, Y., Hosoda, K., Kawada, T., Fushiki, T., and Nakao, K. (1995) Augmented Expression of the Obese Gene in the Adipose Tissue from Rats Fed High-Fat Diet, Biochem. Biophys. Res. Comm. 16, 355–358.
Surwit, R.S., Petro, A.E., Parekh, P., and Collins, S. (1997) Low Plasma Leptin in Response to Dietary Fat in Diabetes- and Obesity-Prone Mice, Diabetes 46, 1516–1520.
De Schepper, J., Zhou, X., De Bock, S., Smitz, J., Louis, O., Hooghe-Peters, E., and Vandenplas, Y. (1998) Study of Serum Leptin in Cafeteria-Diet-Overfed Rats, Horm. Res. 50, 271–275.
Ainslie, D.A., Proietto, J., Fam, B.C., and Thorburn, A.W. (2000) Short-Term, High-Fat Diets Lower Circulating Leptin Concentrations in Rats, Am. J. Clin. Nutr. 71, 438–442.
Storlien, L.H., Kraegen, E.W., Chisholm, D.J., Ford, G.L., Bruce, D.G., and Pascoe, W.S. (1987) Fish Oil Prevents Insulin Resistance Induced by High-Fat Feeding in Rats, Science 237, 885–888.
Hofmann, C., Lorenz, K., Braithwaite, S.S., Colca, J.R., Palazuk, B.J., Hotamisligil, G.S., and Spiegelman, B.M. (1994) Altered Gene Expression for Tumor Necrosis Factor-α and Its Receptors During Drug and Dietary Modulation of Insulin Resistance, Endocrinology 134, 264–270.
Bligh, E.G., and Dyer, W.J. (1959) A Rapid Method for Total Lipid Extraction and Purification, Can. J. Biochem. Physiol. 37, 911–917.
Wong, H., Anderson, W.D., Cheng, T., and Riabowol, K.T. (1994) Monitoring mRNA Expression by Polymerase Chain Reaction: The “Primer-Dropping” Method, Anal. Biochem. 223, 251–258.
Jensen, M.D., Moller, N., Nair, K.S., Eisenberg, P., Landt, M., and Klein, S. (1999) Regional Leptin Kinetics in Humans, Am. J. Clin. Nutr. 69, 18–21.
Bradley, R.L., Cleveland, K.A., and Cheatham, B. (2001) The Adipocyte as a Secretory Organ: Mechanisms of Vesicle Transport and Secretory Pathways, Recent Prog. Horm. Res. 56, 329–358.
Cha, M.C., and Jones, P.J. (1998) Dietary Fat Type and Energy Restriction Interactively Influence Plasma Leptin Concentration in Rats, J. Lipid Res. 39, 1655–1660.
Takahashi, Y., and Ide, T. (1999) Effect of Dietary Fats Differing in Degree of Unsaturation on Gene Expression in Rat Adipose Tissue, Ann. Nutr. Metab. 43, 86–97.
Becker, D.J., Ongemba, L.N., Brichard, V., Henquin, J.C., and Brichard, S.M. (1995) Diet- and Diabetes-Induced Changes of ob Gene Expression in rat Adipose Tissue, FEBS Lett. 371, 324–328.
Trayhurn, P., Thomas, M.E., Duncan, J.S., and Rayner, D.V. (1995) Effects of Fasting and Refeeding on ob Gene Expression in White Adipose Tissue of Lean and Obese (ob/ob) Mice, FEBS Let. 368, 488–490.
Mizuno, T.M., Bergen, H., Funabashi, T., Kleopoulos, S.P., Zhong, Y.G., Bauman, W.A., and Mobbs, C.V. (1996) Obese Gene Expression: Reduction by Fasting and Stimulation by Insulin and Glucose in Lean Mice, and Persistent Elevation in Acquired (diet-induced) and Genetic (yellow agouti) Obesity, Proc. Natl. Acad. Sci. USA 93, 3434–3438.
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Gregersen, S., Thomsen, J.L., Jeppesen, P.B. et al. Impact of Dietary FA and Energy Restriction on Plasma Leptin and ob Gene Expression in Mice. Lipids 38, 513–517 (2003). https://doi.org/10.1007/s11745-003-1092-y
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DOI: https://doi.org/10.1007/s11745-003-1092-y