Lipids

, Volume 38, Issue 1, pp 9–13 | Cite as

Effects of intermittent cycle exercise on intramyocellular lipid use and recovery

  • Lesley J. White
  • Robert A. Robergs
  • Wilmer L. SibbittJr.
  • Michael A. Ferguson
  • Sean McCoy
  • William M. Brooks
Articles

Abstract

The purpose of this investigation was to compare intramyocellular lipid (IMCL) changes in skeletal muscle in nine moderately trained subjects after 45 min of interval cycling and through 1 h of recovery. The exercise session was continous with alternating cycling intensity achieving 50 (3 min) and 110% (2 min) of ventilatory threshold. Spectra from the vastus lateralis were acquired before, immediately after, and 60 min following exercise using a 1.5 T Signa whole-body magnet (point-resolved spectroscopy sequence, echo time 60 ms, transverse relaxation time 2000 ms, 128 acquisitions, and 20 mm3 voxel). Immediately following exercise, IMCI concentration decreased 38% compared to pre-exercise levels (P<0.05). Fitness level and baseline IMCL were not correlated with changes in IMCL following exercise (P>0.05). In the 60-min recovery, IMCL was reduced 30% compared to baseline (P<0.05) and did not recover. In contrast, a nonexercising control group showed no change in IMCL. Our results suggest that IMCL decreased significantly following 45 min of interval cycling, with little recovery in the hour following.

Abbreviations

aHSL

adipocyte hormone-sensitive lipase

AU

arbitrary units

BF

body fat

EMCL

extramyocellular lipid

IMCL

intramyocellular lipid

mHSL

muscle hormone-sensitive lipase

H MRS

proton magnetic resonance spectroscopy

RER

respiratory exchange ratio

VE

minute ventidation

VCO2

carbon dioxide production

VO2

oxygen consumption

VO2max

maximal oxygen consumption

VT

ventilatory threshold

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References

  1. 1.
    Boesch, C., Slotboom, J., Hoppeler, H., and Kreis, R. (1997) In vivo Determination of Intramyocellular Lipids in Human Muscle by means of Localized 1H-MR Spectroscopy, Magn. Reson. Med. 37, 484–493.PubMedGoogle Scholar
  2. 2.
    Szcepaniak, L.S., Babcock, E.E., Schick, F., Dobbins, R.L., Garg, A., Burns, D.K., McGarry, J.D., and Stein, D.T. (1999) Measurement of Intracellular Triglyceride Stores by 1H Spectroscopy: Validation in vivo, Am. J. Physiol. 276, E977–E989.Google Scholar
  3. 3.
    Bessesen, D.H., Rupp, C.L., and Eckel, R.H. (1995) Trafficking of Dietary Fat in Lean Rats, Obes. Res. 3, 191–202.PubMedGoogle Scholar
  4. 4.
    Romijn, J.A., Coyle, E.F., Sidossis, L.S., Gastaldelli, A., Horowitz, J.F., Endert, E., and Wolfe, R.R. (1993) Regulation of Endogenous Fat and Carbohydrate Metabolism in Relation to Exercise Intensity and Duration, Am. J. Physiol. 28, E380-E391.Google Scholar
  5. 5.
    Hurley, B.F., Nemeth, P.M., Martin, W.H., Hagberg, J.M., Dalsky, G.P., and Holloszy, J.O. (1986) Muscle Triglyceride Utilization During Exercise: Effect of Training, J. Appl. Physiol. 60, 562–567.PubMedGoogle Scholar
  6. 6.
    Jansson, E., and Kaijser, L. (1987) Substrate Utilization and Enzyme in Skeletal Muscle of Extremely Endurance-Trained Men. J. Appl. Physiol. 62, 999–1005.PubMedGoogle Scholar
  7. 7.
    Brechtel, K., Niess, A.M., Machann, J., Rett, K., Schick, F., Claussen, C.D., Dickuth, H.H., Haering, H.U., and Jacobs, S. (2001) Utilisation of Intramyocellular Lipids (IMCLs) During Exercise As Assessed by Proton Magnetic Resonance Spectroscopy (1H-MRS), Horm. Metab. Res. 33, 63–66.PubMedCrossRefGoogle Scholar
  8. 8.
    Krssak, M., Petersen, K.F., Bergeron, R., Price, T., Laurent, D., Rothman, D.L., Roden, M., and Shulman, G.I. (2000) Intramuscular Glycogen and Intramuscular Lipid Utilization During Prolonged Exercise and Recovery in Man: A 13C and 1H Nuclear Magnetic Resonance Spectroscopy Study, J. Clin. Endocrin. Metab. 85, 748–754.CrossRefGoogle Scholar
  9. 9.
    Larson-Meyer, D.E., Newcomer, B.R., and Hunter, G.R. (2002) Influence of Endurance Running and Recovery Diet on Intramyocellular Lipid Content in Women: A 1H NMR Study, Am. J. Physiol. 282, E95–E106.Google Scholar
  10. 10.
    Rico-Sanz, J., Hajnal, J.V., Thomas, E.L., Mierisova, S., Ala-Korpela, M., and Bell, J.D. (1998) Intracellular and Extracellular Skeletal Muscle Triglyceride Metabolism During Alternative Intensity Exercise in Humans, J. Physiol. 510, 615–622.PubMedCrossRefGoogle Scholar
  11. 11.
    Rico-Sanz, J., Moosavi, M., Thomas, E.J., McCarthy, J., Coutts, G., Saeed, N., and Bell, J.D. (2000) In vivo Evaluation of the Effects of Continous Exercise on Skeletal Muscle Triglyceride in Trained Humans, Lipids 35, 1313–1317.PubMedCrossRefGoogle Scholar
  12. 12.
    Wendling, P.S., Peters, S.J., Heigenhauser, G.J.F., and Spriet, L.L. (1996) Variability of Triacylglcenol Content in Human Muscle Biopsy Samples, J. Appl. Physiol. 81, 1150–1155.PubMedGoogle Scholar
  13. 13.
    Taylor, H.L., Buskirk, E., and Henchel, A. (1955) Maximal Oxygen Intake as an Objective Measure of Cardiorespiratory Performance, J. Appl. Physiol. 8, 73–80.PubMedGoogle Scholar
  14. 14.
    Caiozzo, V.J., Davis, J.A., Ellis, J.F., Azus, J.L., Vandagriff, R., Prietto, C.A., and McMaster, W.C. (1982) A Comparison of Gas Exchange Indices Used to Detect the Anaerobic Threshold, J. Appl. Physiol. 53, 1184–1189.PubMedGoogle Scholar
  15. 15.
    Jackson, A.S., and Pollock, M.L. (1977) Prediction Accuracy of Body Density, Lean Body Weight, and Total Body Volume Equations, Med. Sci. Sports Exerc 9, 197–201.Google Scholar
  16. 16.
    Shvartz, E., and Reibold, R.C. (1990) Aerobic Fitness Norms for Males and Females Aged 6 to 75 Years: A Review, Avial. Space Environ. Med. 61, 3–11.Google Scholar
  17. 17.
    Kiens, B., Lithnell, H., Mikenes, J., and Richter, E.A. (1989) Effects of Insulin and Exercise on Muscle Lipoprotein Lipase Activity in Man and Its Relation to Insulin Activity, J. Clin. Invest. 84, 1124–1129.PubMedCrossRefGoogle Scholar
  18. 18.
    Starling, R.D., Trappe, T.A., Parcell, A.C., Kerr, A.C., Fink, W.J., and Costill, D.L. (1997) Effects of Diet on Muscle Triglyceride and Endurance Performance, J. Appl. Physiol. 82, 1185–1189.PubMedGoogle Scholar
  19. 19.
    Holm, C.P., Belfrage, P., and Fredrickson, G. (1987) Immunological Evidence for the Presence of Hormone-Sensitive Lipase in Rat Tissue Other Than Adipose Tissue, Biochem. Biophys. Res. Com. 148, 99–105.PubMedCrossRefGoogle Scholar
  20. 20.
    Holm, C.P., Kichgessner, T.G., Svenson, K.L., Fredrickson, G., Nilsson, S., Miller, C.G., Shivey, J.E., Heinzmann, C., Sparkes, R.S., Mohandas, T., et al. (1988) Hormone-Sensitive Lipase: Sequence, Expression and Chromosomal Localization to 19 Cent-q13.3, Science 241, 1503–1506.PubMedCrossRefGoogle Scholar
  21. 21.
    Hales, C.N., Luzio, J.P., and Liddle, K. (1978) Hormonal Control of Adipose Tissue Lipolysis, Biochem. Soc. Symp. 43, 97–135.PubMedGoogle Scholar
  22. 22.
    Kjaer, M., and Secher, N.H. (1992) Neural Influence on Cardiovascular and Endocrine Response to Static Exercise in Humans, Sports Med. 13, 303–319.PubMedCrossRefGoogle Scholar
  23. 23.
    Christmas, M.A., Dawson, B., and Arthur, P.G. (1999) Effect of Work and Recovery Duration on Skeletal Muscle Oxygenation and Fuel Use During Sustained Intermittent Exercise, Eur. J. Appl. Physiol. 80, 436–447.CrossRefGoogle Scholar
  24. 24.
    Kreis, R., Jung, B., Rotman, S., Slortboom, J., and Boesch, C. (1999) Non-invasive Observation of Acetyl-Group Buffering by 1H-MR Spectroscopy in Exercising Human Muscle, NMR Biomed 12, 471–476.PubMedCrossRefGoogle Scholar
  25. 25.
    Holloszy, J.O., Kohrt, W.M., and Hansen, P.A. (1998) The Regulation of Carbohydrate and Fat Metabolism During and After Exercise, Front. Biosci. 3, D1011–D1027.PubMedGoogle Scholar
  26. 26.
    Winder, W.W. (1998) Intramuscular Mechanisms Regulating Fatty Acid Oxidation During Exercise, Adv. Exp. Med. Biol. 441, 239–248.PubMedGoogle Scholar
  27. 27.
    Oscai, L.B., Essig, D.A., and Palmer, W.K. (1990) Lipase Regulation of Muscle Triglyceride Hydrolysis, J. Appl. Physiol. 69, 1571–1577.PubMedGoogle Scholar
  28. 28.
    Guo, Z., and Jensen, M.D. (1999) Blood Glycerol Is an Important Precursor for Intramuscular Triacylglycerol Synthesis, J. Biol. Chem. 274, 702–706.Google Scholar

Copyright information

© AOCS Press 2003

Authors and Affiliations

  • Lesley J. White
    • 1
    • 3
    • 5
  • Robert A. Robergs
    • 1
  • Wilmer L. SibbittJr.
    • 2
    • 3
  • Michael A. Ferguson
    • 5
  • Sean McCoy
    • 3
  • William M. Brooks
    • 2
    • 4
    • 6
  1. 1.Human Performance LaboratoryUniversity of New MexicoAlbuquerque
  2. 2.Clinical and Magnetic Resonance Research CenterUniversity of New MexicoAlbuquerque
  3. 3.Department of Internal MedicineUniversity of New MexicoAlbuquerque
  4. 4.Department NeurosciencesUniversity of New MexicoAlbuquerque
  5. 5.Department of Exercise & Sport SciencesUniversity of FloridaGainesville
  6. 6.Hoglund Brain Imaging CenterUniversity of Kansas Medical CenterKansas City

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